P87074 (RHP9_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair protein rhp9

Alternative name(s):
RAD9 homolog

Gene names

Name:	rhp9
Synonyms:	crb2
ORF Names:	SPBC342.05

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	778 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for cell cycle arrest at the G1 and G2 stages following DNA damage by X-, and UV-irradiation, or inactivation of DNA ligase. Plays a role in the response to DNA damage. Ref.1 Ref.2 Ref.5
Subunit structure	Interacts with sad1. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Ref.4 Ref.7
Subcellular location	Nucleus. Note: Recruited to sites of DNA damage, such as double stand breaks. Ref.2 Ref.4 Ref.5
Sequence similarities	Contains 1 BRCT domain.

Ontologies

Keywords
Biological process	Cell cycle DNA damage
Cellular component	Nucleus
Molecular function	DNA replication inhibitor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitotic G2 DNA damage checkpoint Inferred from mutant phenotype Ref.1. Source: PomBase negative regulation of DNA replication Inferred from electronic annotation. Source: UniProtKB-KW telomere maintenance Inferred from mutant phenotype PubMed 10373582. Source: PomBase
Cellular_component	nuclear chromatin Inferred from sequence model. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		3	EBI-768448,EBI-768448
chk1	P34208	4	EBI-768448,EBI-768535
rad3	Q02099	3	EBI-768448,EBI-768555
rad4	P32372	2	EBI-768448,EBI-768521

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 778

778

DNA repair protein rhp9

PRO_0000097328

Regions

Domain

535 – 653

119

BRCT

Region

376 – 403

28

Interaction with dimethylated histone H4 By similarity

Amino acid modifications

Modified residue

215

1

Phosphothreonine Ref.6

Experimental info

Mutagenesis

215

1

T → A: Reduces hyper-phosphorylation in response to DNA damage. Impairs checkpoint response after DNA damage. Ref.6

Sequence conflict

76

1

F → C in BAA13093. Ref.2

Sequence conflict

84

1

F → C in BAA13093. Ref.2

Sequence conflict

162 – 163

2

LK → YR in BAA13093. Ref.2

Secondary structure

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778

Helix Strand Turn

Details...

Helix

362 – 364

3

Beta strand

365 – 369

5

Beta strand

372 – 374

3

Beta strand

377 – 386

10

Beta strand

395 – 400

6

Beta strand

405 – 409

5

Beta strand

412 – 416

5

Beta strand

423 – 426

4

Beta strand

434 – 440

7

Helix

449 – 451

3

Beta strand

459 – 464

6

Beta strand

467 – 472

6

Helix

473 – 475

3

Beta strand

476 – 478

3

Helix

480 – 483

4

Turn

539 – 542

4

Beta strand

543 – 547

5

Helix

558 – 567

10

Helix

578 – 580

3

Turn

584 – 587

4

Helix

599 – 603

5

Beta strand

605 – 610

6

Helix

618 – 626

9

Helix

634 – 642

9

Helix

649 – 651

3

Beta strand

652 – 658

7

Turn

659 – 662

4

Beta strand

663 – 666

4

Helix

678 – 684

7

Turn

688 – 691

4

Beta strand

693 – 696

4

Helix

714 – 726

13

Beta strand

730 – 732

3

Beta strand

743 – 746

4

Beta strand

748 – 750

3

Helix

763 – 772

10

Sequences

	Sequence		Length	Mass (Da)
	P87074 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 5E35178828E6E42A	FASTA	778	87,462
	10 20 30 40 50 60 MEVNDTSLHK GFGLDINSQR VFGAQAAISR NNYSKVNASI NPSPPRSNDN SNKEFSYSKD 70 80 90 100 110 120 VNNNGAVEEL SLTQLFEVPS QAAFAKQSSQ DISDDELIQH DSRKVISSPY SPKQTHTVLK 130 140 150 160 170 180 RLYDRQSVIS DHEKLLTPQN VSNSSQILSP FTSLLPSTLS TLKDTPLSVS QNEKNLETVG 190 200 210 220 230 240 EVLVPETVAQ HRTKFYDYTL DEMENETESG QVETTPTRLA TSLGSPVLYG RVESTPPAFL 250 260 270 280 290 300 PETSEKQYKR KFSFTEPSSE KVDNTETKFS KKTKNINDEN FPNPFNVISS YETSASPSTV 310 320 330 340 350 360 IDQSSQVSSI FVNKRLRKSV NNQAISRSDS LSLDTPKIDS LFTRASIKPL KPSQSPNSRR 370 380 390 400 410 420 SFKNRVLAFF KGYPSFYYPA TLVAPVHSAV TSSIMYKVQF DDATMSTVNS NQIKRFFLKK 430 440 450 460 470 480 GDVVQSTRLG KIKHTVVKTF RSTNEQLSLI AVDALNNDMV ILAHGEIEVT VPISTIYVAP 490 500 510 520 530 540 VNIRRFQGRD LSFSTLKDMK FEETSFLPSH DSQRNRSSLK ERDSSFVKKN LDSESNQLIF 550 560 570 580 590 600 DDCVFAFSGP VHEDAYDRSA LETVVQDHGG LVLDTGLRPL FNDPFKSKQK KLRHLKPQKR 610 620 630 640 650 660 SKSWNQAFVV SDTFSRKVKY LEALAFNIPC VHPQFIKQCL KMNRVVDFSP YLLASGYSHR 670 680 690 700 710 720 LDCTLSQRIE PFDTTDSLYD RLLARKGPLF GKKILFIIPE AKSWQKKIEN TEQGQKALAH 730 740 750 760 770 VYHALALGAD VEIRPNVAHL ECDLILTMDG NIVDETNCPV VDPEWIVECL ISQSDIST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the Schizosaccharomyces pombe rhp9 gene: a gene required for the DNA damage checkpoint but not the replication checkpoint." Willson J., Wilson S., Warr N., Watts F.Z. Nucleic Acids Res. 25:2138-2145(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: 972 / ATCC 24843.
[2]	"Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1." Saka Y., Esashi F., Matsusaka T., Mochida S., Yanagida M. Genes Dev. 11:3387-3400(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION. Strain: 972 / ATCC 24843.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[4]	"Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast." Miki F., Kurabayashi A., Tange Y., Okazaki K., Shimanuki M., Niwa O. Mol. Genet. Genomics 270:449-461(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SAD1, SUBCELLULAR LOCATION.
[5]	"Methylation of histone H4 lysine 20 controls recruitment of Crb2 to sites of DNA damage." Sanders S.L., Portoso M., Mata J., Baehler J., Allshire R.C., Kouzarides T. Cell 119:603-614(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[6]	"Cooperative control of Crb2 by ATM family and Cdc2 kinases is essential for the DNA damage checkpoint in fission yeast." Nakamura T.M., Moser B.A., Du L.-L., Russell P. Mol. Cell. Biol. 25:10721-10730(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT THR-215, MUTAGENESIS OF THR-215.
[7]	"Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair." Botuyan M.V., Lee J., Ward I.M., Kim J.-E., Thompson J.R., Chen J., Mer G. Cell 127:1361-1373(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 358-507, INTERACTION WITH HISTONE H4.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09431 Genomic DNA. Translation: CAA70582.1.
D86478 Genomic DNA. Translation: BAA13093.1.
CU329671 Genomic DNA. Translation: CAB46775.1.

PIR

T43223.
T45221.

RefSeq

NP_596748.1. NM_001023768.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FHD	X-ray	2.40	A/B/C	358-507	[»]
2VXB	X-ray	2.30	A/B	538-778	[»]
2VXC	X-ray	3.10	A/B	537-778	[»]

ProteinModelPortal

P87074.

SMR

P87074. Positions 358-505, 538-778.

ModBase

Search...

Protein-protein interaction databases

IntAct

P87074. 5 interactions.

MINT

MINT-1179460.

STRING

4896.SPBC342.05-1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

SPBC342.05.1; SPBC342.05.1:pep; SPBC342.05.

GeneID

2540992.

KEGG

spo:SPBC342.05.

Organism-specific databases

PomBase

SPBC342.05.

Phylogenomic databases

eggNOG

NOG323789.

OrthoDB

EOG4FXVHQ.

Family and domain databases

InterPro

IPR001357. BRCT_dom.
[Graphical view]

Pfam

PF00533. BRCT. 1 hit.
[Graphical view]

SMART

SM00292. BRCT. 1 hit.
[Graphical view]

SUPFAM

SSF52113. BRCT. 1 hit.

PROSITE

PS50172. BRCT. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P87074.

NextBio

20802107.

Entry information

Entry name

RHP9_SCHPO

Accession

Primary (citable) accession number: P87074
Secondary accession number(s): Q09754

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program