Reviewed, UniProtKB/Swiss-Prot P87073 (CHS1_PHYBL)

Names and origin

Protein names

Recommended name:
    Chitin synthase 1
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 1
    Class-II chitin synthase 1

Gene names

Name:

chs1

Organism

Phycomyces blakesleeanus

Taxonomic identifier

4837 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Phycomyces

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 841	841	Chitin synthase 1		PRO_0000193708
Regions
	Transmembrane	302 – 322	21	Potential
	Transmembrane	385 – 405	21	Potential
	Transmembrane	526 – 546	21	Potential
	Transmembrane	564 – 584	21	Potential
	Transmembrane	602 – 622	21	Potential
	Transmembrane	644 – 664	21	Potential
	Transmembrane	673 – 693	21	Potential
	Transmembrane	778 – 798	21	Potential
	Transmembrane	816 – 836	21	Potential
Experimental info
	Sequence conflict	179	1	E → EDE Ref.2
	Sequence conflict	199 – 200	2	RV → HI Ref.2
	Sequence conflict	352	1	A → G Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P87073-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 71CD6C09ACB66B8B	FASTA	841	95,227
	10 20 30 40 50 60 MNPGQKQEHD QYPLHDTQFV PQQMDRNSPF ADPYPEDQPP PSGYDHQPLL RDNAPSYPPD 70 80 90 100 110 120 PFGQPGGYPP QSTMYPPQPM GPPSPNMRYG EAPRRQPRRY KTTRRVKLTH GNLILDCPVP 130 140 150 160 170 180 TPYLQAVPIK DTKEFTHMRY TAATCDPADF ASQGYTLRQP ILQRNTELFI VLTMYNEDEI 190 200 210 220 230 240 LFARTMHGVM KNIAHLCSRV RSNVWEGPKA WEKVVVCIVS DGRKKIHPRT LSLLATLGVY 250 260 270 280 290 300 QDGVAKNVVG DKPVTAHIYE YTTQLSVDPE MKFKGADKGM PPCQILFCLK ENNQKKINSH 310 320 330 340 350 360 RWFFQAFGPV INPNVCVLID VGTRPGKTSI YHLWKAFDIS SNIAGACGEI RAMSGTAGVA 370 380 390 400 410 420 LLNPLVAAQN FEYKMSNILD KPLESVFGYI SVLPGAFSAY RFTALQNDEN GHGPLEKYFL 430 440 450 460 470 480 GESQHGADAD IFTANMYLAE DRILCYELVA KKKANWVLHY VSSSYGETDV PDSVPEFISQ 490 500 510 520 530 540 RRRWLNGSFF AGCYALFHWR KVWASDHSFV RKLMFMFEDL YNTYNLIFSW FALGNFYLTF 550 560 570 580 590 600 YILTSALGAE SLDPKPFSAN VASILHTILN YIYILLIIVQ FILALGNRPQ GSKWAYFGSM 610 620 630 640 650 660 TFFAILMVYM MFATIWITVV GVQDAVANAD GSFTAMLGES TFRNIIISIV STYAMYFIAS 670 680 690 700 710 720 FLFFDPWHMF TSFIQYIFLS PSYTNILNIY AFCNTHDVSW GTKGDNTVST DLGVVKSKKD 730 740 750 760 770 780 GSGDTTVEVE VPTEQKDINE AYEEACVELT RQVEPEVSHR DAKTKQEDYY RSFRTRLVIS 790 800 810 820 830 840 WIISNLILVV LITNENILAS FGTFEVRSTS YLGFVLWSVA GLSAIRFCGS GLYLIFRIFM G

References

[1]	Miyazaki A., Ootaki T. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL 1555.
[2]	"Chitin synthase-encoding gene(s) of the Zygomycete fungus Phycomyces blakesleeanus." Miyazaki A., Momany M., Szaniszlo P.J., Jayaram M., Ootaki T. Gene 134:129-134(1993) [PubMed: 8244024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 172-370. Strain: NRRL 1555.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003043 Genomic DNA. Translation: BAA19857.1.
PIR	JT0767.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Enzyme and pathway databases
BRENDA	2.4.1.16. 278.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_PHYBL

Accession

Primary (citable) accession number: P87073

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)