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P87064

- DODA_AMAMU

UniProt

P87064 - DODA_AMAMU

Protein

DOPA 4,5-dioxygenase

Gene

DODA

Organism
Amanita muscaria (Fly agaric)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (19 Feb 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Can also catalyze the formation of muscaflavin (a pigment found in the hygrocybe mushrooms family and of some amanita species only) by a 2,3-extradiol cleavage of DOPA.

    Pathwayi

    GO - Molecular functioni

    1. dioxygenase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Enzyme and pathway databases

    UniPathwayiUPA00278.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DOPA 4,5-dioxygenase (EC:1.14.99.-)
    Gene namesi
    Name:DODA
    OrganismiAmanita muscaria (Fly agaric)
    Taxonomic identifieri41956 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAmanitaceaeAmanita

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 228228DOPA 4,5-dioxygenasePRO_0000079972Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed at high level in coloured cap tissue and at least 10 times lower level in the stipe.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP87064.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Family and domain databases

    Gene3Di3.30.70.1240. 1 hit.
    InterProiIPR023389. DOPA-like.
    IPR014980. DOPA_dioxygen.
    [Graphical view]
    PfamiPF08883. DOPA_dioxygen. 1 hit.
    [Graphical view]
    SUPFAMiSSF143410. SSF143410. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P87064-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVPSFVVYSS WVNGRQRYIR QAFASILFYI IRDTTLSFPS HTTMSTKPET    50
    DLQTVLDSEI KEWHFHIYFH QNNAAEHQAA LELRDAVLRL RQDGAFVAVP 100
    LFRVNMDPMG PHPVGSYEIW VPSETFASVF SYLCMNRGRL SILVHPLTRE 150
    ELRDHEIRNA WIGPSFPLNL ANLPIKSDEI PLQYPSLKLG YSSTAHKMSL 200
    EERRKLGDDI EAVLRGEKEA ARAPHRDA 228
    Length:228
    Mass (Da):26,176
    Last modified:July 1, 1997 - v1
    Checksum:iC03CF0F055026E10
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y12886 Genomic DNA. Translation: CAA73387.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Pretty pigments - Issue 1 of September 2000

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y12886 Genomic DNA. Translation: CAA73387.1 .

    3D structure databases

    ProteinModelPortali P87064.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00278 .

    Family and domain databases

    Gene3Di 3.30.70.1240. 1 hit.
    InterProi IPR023389. DOPA-like.
    IPR014980. DOPA_dioxygen.
    [Graphical view ]
    Pfami PF08883. DOPA_dioxygen. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143410. SSF143410. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The gene coding for the DOPA dioxygenase involved in betalain biosynthesis in Amanita muscaria and its regulation."
      Hinz U.G., Fivaz J., Girod P.-A., Zryd J.-P.
      Mol. Gen. Genet. 256:1-6(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 90-103; 140-158; 178-188 AND 206-215.
      Tissue: Cap.
    2. "Biochemical complementation of the betalain biosynthetic pathway in Portulaca grandiflora by a fungal 3,4-dihydroxyphenylalanine dioxygenase."
      Mueller L.A., Hinz U.G., Uze M., Sautter C., Zryd J.-P.
      Planta 203:260-263(1997)
      Cited for: CHARACTERIZATION.
    3. "The formation of betalamic acid and muscaflavin by recombinant DOPA-dioxygenase from amanita."
      Mueller L.A., Hinz U.G., Zryd J.-P.
      Phytochemistry 44:567-569(1997)
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiDODA_AMAMU
    AccessioniPrimary (citable) accession number: P87064
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 1, 1997
    Last modified: February 19, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

    External Data

    Dasty 3