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P87064 (DODA_AMAMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DOPA 4,5-dioxygenase

EC=1.14.99.-
Gene names
Name:DODA
OrganismAmanita muscaria (Fly agaric)
Taxonomic identifier41956 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAmanitaceaeAmanita

Protein attributes

Sequence length228 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Can also catalyze the formation of muscaflavin (a pigment found in the hygrocybe mushrooms family and of some amanita species only) by a 2,3-extradiol cleavage of DOPA.

Pathway

Pigment biosynthesis; betalain biosynthesis.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed at high level in coloured cap tissue and at least 10 times lower level in the stipe.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionDioxygenase
Oxidoreductase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondioxygenase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 228228DOPA 4,5-dioxygenase
PRO_0000079972

Sequences

Sequence LengthMass (Da)Tools
P87064 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: C03CF0F055026E10

FASTA22826,176
        10         20         30         40         50         60 
MVPSFVVYSS WVNGRQRYIR QAFASILFYI IRDTTLSFPS HTTMSTKPET DLQTVLDSEI 

        70         80         90        100        110        120 
KEWHFHIYFH QNNAAEHQAA LELRDAVLRL RQDGAFVAVP LFRVNMDPMG PHPVGSYEIW 

       130        140        150        160        170        180 
VPSETFASVF SYLCMNRGRL SILVHPLTRE ELRDHEIRNA WIGPSFPLNL ANLPIKSDEI 

       190        200        210        220 
PLQYPSLKLG YSSTAHKMSL EERRKLGDDI EAVLRGEKEA ARAPHRDA 

« Hide

References

[1]"The gene coding for the DOPA dioxygenase involved in betalain biosynthesis in Amanita muscaria and its regulation."
Hinz U.G., Fivaz J., Girod P.-A., Zryd J.-P.
Mol. Gen. Genet. 256:1-6(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 90-103; 140-158; 178-188 AND 206-215.
Tissue: Cap.
[2]"Biochemical complementation of the betalain biosynthetic pathway in Portulaca grandiflora by a fungal 3,4-dihydroxyphenylalanine dioxygenase."
Mueller L.A., Hinz U.G., Uze M., Sautter C., Zryd J.-P.
Planta 203:260-263(1997)
Cited for: CHARACTERIZATION.
[3]"The formation of betalamic acid and muscaflavin by recombinant DOPA-dioxygenase from amanita."
Mueller L.A., Hinz U.G., Zryd J.-P.
Phytochemistry 44:567-569(1997)
Cited for: CHARACTERIZATION.

Web resources

Protein Spotlight

Pretty pigments - Issue 1 of September 2000

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y12886 Genomic DNA. Translation: CAA73387.1.

3D structure databases

ProteinModelPortalP87064.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00278.

Family and domain databases

Gene3D3.30.70.1240. 1 hit.
InterProIPR023389. DOPA-like.
IPR014980. DOPA_dioxygen.
[Graphical view]
PfamPF08883. DOPA_dioxygen. 1 hit.
[Graphical view]
SUPFAMSSF143410. SSF143410. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDODA_AMAMU
AccessionPrimary (citable) accession number: P87064
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PATHWAY comments

Index of metabolic and biosynthesis pathways