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Protein

DOPA 4,5-dioxygenase

Gene

DODA

Organism
Amanita muscaria (Fly agaric)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Can also catalyze the formation of muscaflavin (a pigment found in the hygrocybe mushrooms family and of some amanita species only) by a 2,3-extradiol cleavage of DOPA.

Pathway: betalain biosynthesis

This protein is involved in the pathway betalain biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway betalain biosynthesis and in Pigment biosynthesis.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Enzyme and pathway databases

UniPathwayiUPA00278.

Names & Taxonomyi

Protein namesi
Recommended name:
DOPA 4,5-dioxygenase (EC:1.14.99.-)
Gene namesi
Name:DODA
OrganismiAmanita muscaria (Fly agaric)
Taxonomic identifieri41956 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAmanitaceaeAmanita

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228DOPA 4,5-dioxygenasePRO_0000079972Add
BLAST

Expressioni

Tissue specificityi

Expressed at high level in coloured cap tissue and at least 10 times lower level in the stipe.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP87064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

Gene3Di3.30.70.1240. 1 hit.
InterProiIPR023389. DOPA-like.
IPR014980. DOPA_dioxygen.
[Graphical view]
PfamiPF08883. DOPA_dioxygen. 1 hit.
[Graphical view]
SUPFAMiSSF143410. SSF143410. 1 hit.

Sequencei

Sequence statusi: Complete.

P87064-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPSFVVYSS WVNGRQRYIR QAFASILFYI IRDTTLSFPS HTTMSTKPET
60 70 80 90 100
DLQTVLDSEI KEWHFHIYFH QNNAAEHQAA LELRDAVLRL RQDGAFVAVP
110 120 130 140 150
LFRVNMDPMG PHPVGSYEIW VPSETFASVF SYLCMNRGRL SILVHPLTRE
160 170 180 190 200
ELRDHEIRNA WIGPSFPLNL ANLPIKSDEI PLQYPSLKLG YSSTAHKMSL
210 220
EERRKLGDDI EAVLRGEKEA ARAPHRDA
Length:228
Mass (Da):26,176
Last modified:July 1, 1997 - v1
Checksum:iC03CF0F055026E10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12886 Genomic DNA. Translation: CAA73387.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

Pretty pigments - Issue 1 of September 2000

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y12886 Genomic DNA. Translation: CAA73387.1.

3D structure databases

ProteinModelPortaliP87064.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00278.

Family and domain databases

Gene3Di3.30.70.1240. 1 hit.
InterProiIPR023389. DOPA-like.
IPR014980. DOPA_dioxygen.
[Graphical view]
PfamiPF08883. DOPA_dioxygen. 1 hit.
[Graphical view]
SUPFAMiSSF143410. SSF143410. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The gene coding for the DOPA dioxygenase involved in betalain biosynthesis in Amanita muscaria and its regulation."
    Hinz U.G., Fivaz J., Girod P.-A., Zryd J.-P.
    Mol. Gen. Genet. 256:1-6(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 90-103; 140-158; 178-188 AND 206-215.
    Tissue: Cap.
  2. "Biochemical complementation of the betalain biosynthetic pathway in Portulaca grandiflora by a fungal 3,4-dihydroxyphenylalanine dioxygenase."
    Mueller L.A., Hinz U.G., Uze M., Sautter C., Zryd J.-P.
    Planta 203:260-263(1997)
    Cited for: CHARACTERIZATION.
  3. "The formation of betalamic acid and muscaflavin by recombinant DOPA-dioxygenase from amanita."
    Mueller L.A., Hinz U.G., Zryd J.-P.
    Phytochemistry 44:567-569(1997)
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiDODA_AMAMU
AccessioniPrimary (citable) accession number: P87064
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: May 27, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.