Reviewed, UniProtKB/Swiss-Prot P87064 (DODA_AMAMU)

Names and origin

Protein names

Recommended name:
    DOPA 4,5-dioxygenase
    EC=1.14.99.-

Gene names

Name:

DODA

Organism

Amanita muscaria (Fly agaric)

Taxonomic identifier

41956 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Amanitaceae › Amanita

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Extradiol dioxygenase that opens up the cyclic ring of DOPA between carbons 4 and 5 thus producing an unstable seco-DOPA that rearranges non-enzymatically to betalamic acid. Can also catalyze the formation of muscaflavin (a pigment found in the hygrocybe mushrooms family and of some amanita species only) by a 2,3-extradiol cleavage of DOPA.
Pathway	Pigment biosynthesis; betalain biosynthesis.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Tissue specificity	Expressed at high level in coloured cap tissue and at least 10 times lower level in the stipe.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	DOPA 4,5-dioxygenase		PRO_0000079972

Sequences

	Sequence		Length	Mass (Da)
	P87064-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: C03CF0F055026E10	FASTA	228	26,176
	10 20 30 40 50 60 MVPSFVVYSS WVNGRQRYIR QAFASILFYI IRDTTLSFPS HTTMSTKPET DLQTVLDSEI 70 80 90 100 110 120 KEWHFHIYFH QNNAAEHQAA LELRDAVLRL RQDGAFVAVP LFRVNMDPMG PHPVGSYEIW 130 140 150 160 170 180 VPSETFASVF SYLCMNRGRL SILVHPLTRE ELRDHEIRNA WIGPSFPLNL ANLPIKSDEI 190 200 210 220 PLQYPSLKLG YSSTAHKMSL EERRKLGDDI EAVLRGEKEA ARAPHRDA

References

[1]	"The gene coding for the DOPA dioxygenase involved in betalain biosynthesis in Amanita muscaria and its regulation." Hinz U.G., Fivaz J., Girod P.-A., Zryd J.-P. Mol. Gen. Genet. 256:1-6(1997) [PubMed: 9341673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 90-103; 140-158; 178-188 AND 206-215. Tissue: Cap.
[2]	"Biochemical complementation of the betalain biosynthetic pathway in Portulaca grandiflora by a fungal 3,4-dihydroxyphenylalanine dioxygenase." Mueller L.A., Hinz U.G., Uze M., Sautter C., Zryd J.-P. Planta 203:260-263(1997) Cited for: CHARACTERIZATION.
[3]	"The formation of betalamic acid and muscaflavin by recombinant DOPA-dioxygenase from amanita." Mueller L.A., Hinz U.G., Zryd J.-P. Phytochemistry 44:567-569(1997) Cited for: CHARACTERIZATION.

Web resources

Protein Spotlight

Pretty pigments - Issue 1 of September 2000

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y12886 Genomic DNA. Translation: CAA73387.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014980. DOPA_dioxygen. [Graphical view]
Pfam	PF08883. DOPA_dioxygen. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DODA_AMAMU

Accession

Primary (citable) accession number: P87064

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)