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Protein

Trihydroxynaphthalene reductase

Gene

THR1

Organism
Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 1,3,8-trihydroxynaphthalene (T3HN) into (-)-vermelone. Essential for appressorial penetration of colletotrichum lagenarium.

Pathwayi: melanin biosynthesis

This protein is involved in the pathway melanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway melanin biosynthesis and in Pigment biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641SubstrateBy similarity
Active sitei178 – 1781Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 5725NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Melanin biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00785.

Names & Taxonomyi

Protein namesi
Recommended name:
Trihydroxynaphthalene reductase (EC:1.1.1.-)
Alternative name(s):
T3HN reductase
Gene namesi
Name:THR1
ORF Names:Cob_04570
OrganismiColletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum lagenarium)
Taxonomic identifieri1213857 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeGlomerellalesGlomerellaceaeColletotrichum
Proteomesi
  • UP000014480 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Trihydroxynaphthalene reductasePRO_0000054783Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P87025-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVTSQSAG SKYDAIPGPL GLASASLMGK VALVTGAGRG IGREMAMELG
60 70 80 90 100
RRGAKVIVNY ANSAETAEEV VQAIKKSGSD AASIKANVSD VDQIVKMFGE
110 120 130 140 150
AKQIWGRLDI VCSNSGVVSF GHVKDVTPEE FDRVFAINTR GQFFVAREAY
160 170 180 190 200
KHLEVGGRLI LMGSITGQAK GVPKHAVYSG SKGTIETFVR CMAIDFGDKK
210 220 230 240 250
ITVNAVAPGG IKTDMYRDVC REYIPNGGEL DDEGVDEFAA GWSPMHRVGL
260 270 280
PIDIARVVCF LASQDGEWIN GKVLGIDGAA CM
Length:282
Mass (Da):29,849
Last modified:September 18, 2013 - v4
Checksum:i69BDAB12F6DA9B23
GO

Sequence cautioni

The sequence BAA18962.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83988 Genomic DNA. Translation: BAA18962.1. Sequence problems.
KB725704 Genomic DNA. Translation: ENH87277.1.

Genome annotation databases

EnsemblFungiiENH87277; ENH87277; Cob_04570.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83988 Genomic DNA. Translation: BAA18962.1. Sequence problems.
KB725704 Genomic DNA. Translation: ENH87277.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiENH87277; ENH87277; Cob_04570.

Enzyme and pathway databases

UniPathwayiUPA00785.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a melanin biosynthetic THR1 reductase gene essential for appressorial penetration of Colletotrichum lagenarium."
    Perpetua N.S., Kubo Y., Yasuda N., Takano Y., Furusawa I.
    Mol. Plant Microbe Interact. 9:323-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.
  2. "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic stage shift of Colletotrichum fungi."
    Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y., Takano Y., Kubo Y., Shirasu K.
    New Phytol. 197:1236-1249(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422.

Entry informationi

Entry nameiTHR1_COLOR
AccessioniPrimary (citable) accession number: P87025
Secondary accession number(s): N4VTL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: September 18, 2013
Last modified: May 11, 2016
This is version 73 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.