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Protein

5'-hydroxyaverantin dehydrogenase

Gene

adhA

Organism
Aspergillus parasiticus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of 5'-hydroxyaverantin to 5'-oxoaverantin in the aflatoxin biosynthesis pathway. 5'-oxoaverantin spontaneously forms averufin (AVF) by intramolecular ketalisation.2 Publications

Catalytic activityi

(1'S,5'S)-hydroxyaverantin + NAD+ = 5'-oxoaverantin + NADH.1 Publication
(1'S,5'R)-hydroxyaverantin + NAD+ = 5'-oxoaverantin + NADH.1 Publication

Pathwayi: aflatoxin biosynthesis

This protein is involved in the pathway aflatoxin biosynthesis, which is part of Mycotoxin biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway aflatoxin biosynthesis and in Mycotoxin biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei174 – 1741SubstrateBy similarity
Active sitei187 – 1871Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 4325NAD or NADPBy similarityAdd
BLAST

GO - Molecular functioni

  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • aflatoxin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14032.
BRENDAi1.1.1.352. 523.
UniPathwayiUPA00287.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-hydroxyaverantin dehydrogenase (EC:1.1.1.352)
Alternative name(s):
Aflatoxin biosynthesis protein H
HAVN dehydrogenase
Gene namesi
Name:adhA
Synonyms:aflH
OrganismiAspergillus parasiticus
Taxonomic identifieri5067 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Pathology & Biotechi

Disruption phenotypei

Cells accumulate 5'-hydroxyaverantin.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2782785'-hydroxyaverantin dehydrogenasePRO_0000424162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Structurei

3D structure databases

ProteinModelPortaliP87017.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK17652.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 1 hit.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P87017-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVLDTTVDL GTLQGKSALI TGGASGIGLA TARAWAAAGM YVTIADIQPL
60 70 80 90 100
ETGQNILADL AGGHVHYVCC DVTSWESQIT AFKEAIQFTP SKALDIVAAF
110 120 130 140 150
AGVSFAGGNQ VDHVLAAGDP RLDVNPSPPD IRNIQVNLIG VYYTSWLGLY
160 170 180 190 200
YLRLSPTNKA ANPSPDKSLI LMGSIGSYMD SPKASTYPAS KFGVRGLFRS
210 220 230 240 250
TRARTRELGV RCNLLAPWFI DTPLIAPMKK AMAARGIDMA QRLTFASVDA
260 270
CVEAATTCAA NPQLHGTPPI RYAYCLKT
Length:278
Mass (Da):29,557
Last modified:May 1, 2000 - v2
Checksum:i8C60C5437EB9BB71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76621 Genomic DNA. Translation: AAB51228.3.
AY371490 Genomic DNA. Translation: AAS66009.1.

Genome annotation databases

KEGGiag:AAB51228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76621 Genomic DNA. Translation: AAB51228.3.
AY371490 Genomic DNA. Translation: AAS66009.1.

3D structure databases

ProteinModelPortaliP87017.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAB51228.

Phylogenomic databases

KOiK17652.

Enzyme and pathway databases

UniPathwayiUPA00287.
BioCyciMetaCyc:MONOMER-14032.
BRENDAi1.1.1.352. 523.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 1 hit.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "adhA in Aspergillus parasiticus is involved in conversion of 5'-hydroxyaverantin to averufin."
    Chang P.K., Yu J., Ehrlich K.C., Boue S.M., Montalbano B.G., Bhatnagar D., Cleveland T.E.
    Appl. Environ. Microbiol. 66:4715-4719(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
  2. "Completed sequence of aflatoxin pathway gene cluster in Aspergillus parasiticus."
    Yu J., Bhatnagar D., Cleveland T.E.
    FEBS Lett. 564:126-130(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.
  3. "Involvement of two cytosolic enzymes and a novel intermediate, 5'-oxoaverantin, in the pathway from 5'-hydroxyaverantin to averufin in aflatoxin biosynthesis."
    Sakuno E., Yabe K., Nakajima H.
    Appl. Environ. Microbiol. 69:6418-6426(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-33; 122-132; 168-183; 192-199 AND 207-21, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, ACETYLATION AT MET-1.
  4. Cited for: REVIEW.
    Strain: ATCC 56775 / NRRL 5862 / Su-1 / SRRC 143.

Entry informationi

Entry nameiAFLH_ASPPA
AccessioniPrimary (citable) accession number: P87017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: May 1, 2000
Last modified: April 13, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.