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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

mam4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in M-factor.

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.

GO - Molecular functioni

  • protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: PomBase

GO - Biological processi

  • cellular protein localization Source: PomBase
  • peptide mating pheromone maturation involved in posititve regulation of conjugation with cellular fusion Source: PomBase
  • response to pheromone triggering conjugation with cellular fusion Source: PomBase

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processPheromone response
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-SPO-163841 Gamma carboxylation, hypusine formation and arylsulfatase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:mam4
ORF Names:SPAC10F6.12c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC10F6.12c
PomBaseiSPAC10F6.12c mam4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Transmembranei76 – 96HelicalSequence analysisAdd BLAST21
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002098981 – 236Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST236

Proteomic databases

MaxQBiP87014
PaxDbiP87014
PRIDEiP87014

Interactioni

Protein-protein interaction databases

BioGridi279420, 1 interactor
STRINGi4896.SPAC10F6.12c.1

Structurei

3D structure databases

SMRiP87014
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000213961
InParanoidiP87014
KOiK00587
OMAiFRTIAMY
OrthoDBiEOG092C5DVA
PhylomeDBiP87014

Family and domain databases

InterProiView protein in InterPro
IPR007269 ICMT_MeTrfase
IPR025770 PPMT_MeTrfase
PfamiView protein in Pfam
PF04140 ICMT, 1 hit
PROSITEiView protein in PROSITE
PS51564 SAM_ICMT, 1 hit

Sequencei

Sequence statusi: Complete.

P87014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNLHTSIAV ASICLTSAFL GCVFGLGFFV WIIYGYSIGG FFAFLSLFHL
60 70 80 90 100
LEFYITARFQ GSQLSWDSFI LNNGKAYWLA MLVGLLECLL SGGKSFAKVI
110 120 130 140 150
NCLRFPSFLI NFIFSVYQTS ALGFLCLGQY LRSSAMVQAG QSFSHIVASK
160 170 180 190 200
RNKDHLLVTD GIYAYVRHPS YVGFFIWALG TQMLLGNFVS TLLFSLVLWK
210 220 230
FFSQRITTEE AYLVSFFGDS YEQYRKKVPS GIPLIP
Length:236
Mass (Da):26,505
Last modified:July 1, 1997 - v1
Checksum:iBE22A8C80BAA16FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87749 Genomic DNA Translation: BAA18999.1
CU329670 Genomic DNA Translation: CAA15725.1
PIRiT43237
RefSeqiNP_593263.1, NM_001018660.2

Genome annotation databases

EnsemblFungiiSPAC10F6.12c.1; SPAC10F6.12c.1:pep; SPAC10F6.12c
GeneIDi2542982
KEGGispo:SPAC10F6.12c

Similar proteinsi

Entry informationi

Entry nameiMAM4_SCHPO
AccessioniPrimary (citable) accession number: P87014
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 1, 1997
Last modified: April 25, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health