P87014 (MAM4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase
EC=2.1.1.100

Alternative name(s):
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name=PPMT
Prenylcysteine carboxyl methyltransferase
Short name=pcCMT

Gene names

Name:	mam4
ORF Names:	SPAC10F6.12c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mediates C-terminal methylation of the isoprenylated C-terminal cysteine in M-factor.
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein localization Inferred from mutant phenotype Ref.1. Source: PomBase peptide mating pheromone maturation involved in conjugation with cellular fusion Inferred from mutant phenotype Ref.1. Source: PomBase peptidyl-L-cysteine methyl ester biosynthetic process from peptidyl-cysteine Inferred from mutant phenotype Ref.1. Source: PomBase peptidyl-cysteine methylation Inferred from mutant phenotype Ref.1. Source: PomBase response to pheromone involved in conjugation with cellular fusion Inferred by curator. Source: PomBase
Cellular_component	endoplasmic reticulum membrane Inferred from sequence orthology. Source: PomBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	Protein-S-isoprenylcysteine O-methyltransferase		PRO_0000209898
Regions
	Transmembrane	3 – 23	21	Helical; Potential
	Transmembrane	24 – 44	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	108 – 128	21	Helical; Potential
	Transmembrane	174 – 194	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P87014 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: BE22A8C80BAA16FD	FASTA	236	26,505
	10 20 30 40 50 60 MGNLHTSIAV ASICLTSAFL GCVFGLGFFV WIIYGYSIGG FFAFLSLFHL LEFYITARFQ 70 80 90 100 110 120 GSQLSWDSFI LNNGKAYWLA MLVGLLECLL SGGKSFAKVI NCLRFPSFLI NFIFSVYQTS 130 140 150 160 170 180 ALGFLCLGQY LRSSAMVQAG QSFSHIVASK RNKDHLLVTD GIYAYVRHPS YVGFFIWALG 190 200 210 220 230 TQMLLGNFVS TLLFSLVLWK FFSQRITTEE AYLVSFFGDS YEQYRKKVPS GIPLIP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genes encoding farnesyl cysteine carboxyl methyltransferase in Schizosaccharomyces pombe and Xenopus laevis." Imai Y., Davey J., Kawagishi-Kobayashi M., Yamamoto M. Mol. Cell. Biol. 17:1543-1551(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87749 Genomic DNA. Translation: BAA18999.1. CU329670 Genomic DNA. Translation: CAA15725.1.
PIR	T43237.
RefSeq	NP_593263.1. NM_001018660.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4692101.
STRING	4896.SPAC10F6.12c-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC10F6.12c.1; SPAC10F6.12c.1:pep; SPAC10F6.12c.
GeneID	2542982.
KEGG	spo:SPAC10F6.12c.
Organism-specific databases
PomBase	SPAC10F6.12c.
Phylogenomic databases
eggNOG	COG2020.
HOGENOM	HOG000213961.
KO	K00587.
OMA	LAMTKRS.
OrthoDB	EOG4NS6N5.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. IPR025770. PPMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20804015.

Entry information

Entry name

MAM4_SCHPO

Accession

Primary (citable) accession number: P87014

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	July 1, 1997
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program