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Protein

Alcohol dehydrogenase class-3

Gene
N/A
Organism
Scyliorhinus canicula (Small-spotted catshark) (Squalus canicula)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.By similarity

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.By similarity
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47Zinc 1; catalyticBy similarity1
Metal bindingi69Zinc 1; catalyticBy similarity1
Metal bindingi99Zinc 2By similarity1
Metal bindingi102Zinc 2By similarity1
Metal bindingi105Zinc 2By similarity1
Metal bindingi113Zinc 2By similarity1
Sitei117Important for FDH activity and activation by fatty acidsBy similarity1
Metal bindingi176Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BRENDAi1.1.1.1. 5640.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase class-3By similarity (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase class-IIIBy similarity
Glutathione-dependent formaldehyde dehydrogenaseBy similarity (EC:1.1.1.-)
Short name:
FALDHBy similarity
Short name:
FDHBy similarity
Short name:
GSH-FDHBy similarity
S-(hydroxymethyl)glutathione dehydrogenaseBy similarity (EC:1.1.1.284)
OrganismiScyliorhinus canicula (Small-spotted catshark) (Squalus canicula)
Taxonomic identifieri7830 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiGaleomorphiiGaleoideaCarcharhiniformesScyliorhinidaeScyliorhinus

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004053121 – 376Alcohol dehydrogenase class-3Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP86884.
SMRiP86884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiView protein in PROSITE
PS00059. ADH_ZINC. 1 hit.

Sequencei

Sequence statusi: Complete.

P86884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AGTVGKVIKC KAAVAWEAGK PLCIEEIEVA PPKAHEVRIK IIATAVCHSD
60 70 80 90 100
SYTLSGSDSE GLFPVILGHE GAGIVESVGE GVTCVKAGDR VIPLYIPQCG
110 120 130 140 150
DCNFCLNPKT NLCQKIRVTQ GQGLMPDKTS RFTCKGQLLY HYMGTSTFSE
160 170 180 190 200
YTVVADISVA KIDEAAPLDK VCLLGCGIST GYGAAVNTAK VEPGSTCAVF
210 220 230 240 250
GLGGVGLAGI MGCKAAGATR IIGVDLNKDK FTKAKEFGAT EYINPKDYDK
260 270 280 290 300
PIQEVLVGLT DGGVDYSFEC IGNVKTMRAA LEACHKGWGT SVIVGVAPAG
310 320 330 340 350
HEIATRPFQL VTGRTWKGTA FGGWKSVESV PKLVKEYMAK KLKVDEFVTH
360 370
TLPFNQINES FELMHAGKSI RCVLSF
Length:376
Mass (Da):39,937
Last modified:March 8, 2011 - v1
Checksum:i36D56CECA4B3BA12
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP86884.
SMRiP86884.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.1.1.1. 5640.

Family and domain databases

CDDicd08300. alcohol_DH_class_III. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR014183. ADH_3.
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SUPFAMiSSF50129. SSF50129. 2 hits.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR02818. adh_III_F_hyde. 1 hit.
PROSITEiView protein in PROSITE
PS00059. ADH_ZINC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADHX_SCYCA
AccessioniPrimary (citable) accession number: P86884
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 8, 2011
Last modified: March 15, 2017
This is version 25 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.