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P86875 (HUGA_VESMG) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hyaluronidase
Short name=Hya
EC=3.2.1.35
Alternative name(s):
Hyaluronoglucosaminidase
Allergen=Vesp ma 2
OrganismVespa magnifica (Hornet)
Taxonomic identifier202807 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespa

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. UniProtKB Q08169

Subcellular location

Secreted Ref.1 UniProtKB Q08169.

Allergenic properties

Causes an allergic reaction in human. Binds to IgE. Ref.1 Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhyalurononglucosaminidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Ref.1
Chain27 – 357331Hyaluronidase
PRO_0000405128

Sites

Active site1351Proton donor By similarity UniProtKB Q08169

Amino acid modifications

Glycosylation1051N-linked (GlcNAc...) Potential
Glycosylation1251N-linked (GlcNAc...) Potential
Glycosylation1531N-linked (GlcNAc...) Potential
Glycosylation3511N-linked (GlcNAc...) Potential
Disulfide bond45 ↔ 334 By similarity UniProtKB Q08169
Disulfide bond211 ↔ 223 By similarity UniProtKB Q08169

Experimental info

Sequence conflict1601R → P AA sequence Ref.1
Sequence conflict1721E → G AA sequence Ref.1
Sequence conflict3141N → S AA sequence Ref.1
Sequence conflict3351T → M AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
P86875 [UniParc].

Last modified May 3, 2011. Version 2.
Checksum: 709A79B50DEE9A53

FASTA35741,948
        10         20         30         40         50         60 
MLLVTLFLFF LQALVNGDSC GSNCEKSERP KRVFNIYWNV PTFMCHQYGL YFDEVTNFNI 

        70         80         90        100        110        120 
KHNSKDNFQG DKIAIFYDPG EFPALLPLNY GKYKIRNGGV PQEGNITIHL QRFIEYLDKT 

       130        140        150        160        170        180 
YPNRNFSGIG VIDFERWRPI FRQNWGNMKI YKNFSIDLVR KEHPFWNKKM IELEASKRFE 

       190        200        210        220        230        240 
KYARLFMEET LKLAKKTRKQ ADWGYYGYPY CFNMSPTNFV PDCDVTARDE NNEMSWLFNN 

       250        260        270        280        290        300 
QNVLLPSVYI RRELTPDQRI GLVQGRVKEA VRISNKLKHS PKVFSYWWYV YQDETNTFLT 

       310        320        330        340        350 
ETDVKKTFQE IVINGGDGII IWGSSSDVNS LSKCTRLREY LLTVLGPIAV NVTEAVN

« Hide

References

[1]"Purification and characterization of two new allergens from the venom of Vespa magnifica."
An S., Chen L., Wei J.F., Yang X., Ma D., Xu X., Xu X., He S., Lu J., Lai R.
PLoS ONE 7:E31920-E31920(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-54; 73-91; 125-135; 200-220; 283-304 AND 339-357, SUBCELLULAR LOCATION, ALLERGEN.
Tissue: Venom and Venom gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FR749885 mRNA. Translation: CBY83816.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

Allergome9454. Vesp ma 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001329. Glyco_hydro_56_Api/Dol.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERPTHR11769. PTHR11769. 1 hit.
PTHR11769:SF2. PTHR11769:SF2. 1 hit.
PfamPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHUGA_VESMG
AccessionPrimary (citable) accession number: P86875
Secondary accession number(s): E7BBB9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: May 3, 2011
Last modified: March 6, 2013
This is version 11 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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