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Protein

Hyaluronidase

Gene
N/A
Organism
Vespa magnifica (Hornet)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase (EC:3.2.1.35)
Short name:
HyaBy similarity
Alternative name(s):
HyaluronoglucosaminidaseBy similarity
Allergen: Vesp ma 2
OrganismiVespa magnifica (Hornet)
Taxonomic identifieri202807 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespa

Subcellular locationi

Secreted By similarity1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei9454. Vesp ma 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26261 PublicationAdd
BLAST
Chaini27 – 357331HyaluronidasePRO_0000405128Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi45 ↔ 334By similarity
Glycosylationi105 – 1051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi125 – 1251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi211 ↔ 223By similarity
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Sequence Analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P86875-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLVTLFLFF LQALVNGDSC GSNCEKSERP KRVFNIYWNV PTFMCHQYGL
60 70 80 90 100
YFDEVTNFNI KHNSKDNFQG DKIAIFYDPG EFPALLPLNY GKYKIRNGGV
110 120 130 140 150
PQEGNITIHL QRFIEYLDKT YPNRNFSGIG VIDFERWRPI FRQNWGNMKI
160 170 180 190 200
YKNFSIDLVR KEHPFWNKKM IELEASKRFE KYARLFMEET LKLAKKTRKQ
210 220 230 240 250
ADWGYYGYPY CFNMSPTNFV PDCDVTARDE NNEMSWLFNN QNVLLPSVYI
260 270 280 290 300
RRELTPDQRI GLVQGRVKEA VRISNKLKHS PKVFSYWWYV YQDETNTFLT
310 320 330 340 350
ETDVKKTFQE IVINGGDGII IWGSSSDVNS LSKCTRLREY LLTVLGPIAV

NVTEAVN
Length:357
Mass (Da):41,948
Last modified:May 3, 2011 - v2
Checksum:i709A79B50DEE9A53
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601R → P AA sequence (PubMed:22384100)Curated
Sequence conflicti172 – 1721E → G AA sequence (PubMed:22384100)Curated
Sequence conflicti314 – 3141N → S AA sequence (PubMed:22384100)Curated
Sequence conflicti335 – 3351T → M AA sequence (PubMed:22384100)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR749885 mRNA. Translation: CBY83816.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR749885 mRNA. Translation: CBY83816.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei9454. Vesp ma 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Purification and characterization of two new allergens from the venom of Vespa magnifica."
    An S., Chen L., Wei J.F., Yang X., Ma D., Xu X., Xu X., He S., Lu J., Lai R.
    PLoS ONE 7:E31920-E31920(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-54; 73-91; 125-135; 200-220; 283-304 AND 339-357, SUBCELLULAR LOCATION, ALLERGEN.
    Tissue: Venom and Venom gland.

Entry informationi

Entry nameiHUGA_VESMG
AccessioniPrimary (citable) accession number: P86875
Secondary accession number(s): E7BBB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: May 3, 2011
Last modified: January 7, 2015
This is version 16 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.