P86830 (AAE_RAUVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylajmalan esterase
EC=3.1.1.80

Gene names

Name:

AAE

Organism

Rauvolfia verticillata (Common devil-pepper) (Dissolena verticillata)

Taxonomic identifier

329875 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Rauvolfia

Protein attributes

Sequence length	46 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deacetylates 17-O-acetylajmaline and 17-O-acetylnorajmaline, but is inactive toward other acetylated alkaloids By similarity. UniProtKB Q3MKY2
Catalytic activity	17-O-acetylajmaline + H2O = ajmaline + acetate. Ref.1 UniProtKB Q3MKY2 17-O-acetylnorajmaline + H2O = norajmaline + acetate. Ref.1 UniProtKB Q3MKY2
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	acetylajmaline esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›46	›46	Acetylajmalan esterase		PRO_0000402568
Amino acid modifications
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	46	1

Sequences

	Sequence		Length	Mass (Da)
	P86830 [UniParc]. Last modified January 11, 2011. Version 1. Checksum: 7F9778EE939B4CBD	FASTA	46	5,242
	10 20 30 40 NALFILGNIG NNDVNYAFPD RAIEEIRFYV PFITEAVANA TREIIR

References

[1]	"Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system." Ruppert M., Woll J., Giritch A., Genady E., Ma X., Stockigt J. Planta 222:888-898(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS01098. LIPASE_GDSL_SER. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAE_RAUVE

Accession

Primary (citable) accession number: P86830

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2011
Last sequence update:	January 11, 2011
Last modified:	April 5, 2011
This is version 3 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program