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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Siganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the growth of both Gram-negative and Gram-positive bacteria. Displays strong antibacterial activity towards V.cholerae and E.tarda. Causes deformation of the surface of S.aureus and the formation of pores on the surface of E.coli. Strong antiparasitic activity is seen towards C.irritans, T.brucei and I.multifiliis. Cilia of treated theronts are lost and the macronucleus swells, inducing cell membrane rupture and efflux of the cytoplasm.2 Publications

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.By similarity

Cofactori

FADBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei98FADBy similarity1
Binding sitei117SubstrateBy similarity1
Binding sitei288FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei403SubstrateBy similarity1
Binding sitei485FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi70 – 71FADBy similarity2
Nucleotide bindingi90 – 91FADBy similarity2
Nucleotide bindingi114 – 117FADBy similarity4
Nucleotide bindingi492 – 497FADBy similarity6
Nucleotide bindingi492 – 493SubstrateBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Oxidoreductase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase1 Publication (EC:1.4.3.2By similarity)
Alternative name(s):
Antiparasitic protein1 Publication
Short name:
APP1 Publication
Serum L-amino-acid oxidase
Short name:
SR-LAAO1 Publication
OrganismiSiganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
Taxonomic identifieri75042 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataEupercariaSiganidaeSiganus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
ChainiPRO_000040110728 – 527L-amino-acid oxidase1 PublicationAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 200By similarity
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi393N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expression mainly observed in plasma, spleen, kidney and gills with low levels detected in blood and no expression detected in brain, liver, heart, muscle or intestine (at protein level).2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP86810.
SMRiP86810.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the flavin monoamine oxidase family. FIG1 subfamily.Sequence analysis

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P86810-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLHRAPWKS SAAAAVLLLA LFSGAAASSV EKNLAACLRD NDYDQLLQTV
60 70 80 90 100
QDGLPHINTS NHVVIVGAGV AGLTAAKLLQ DAGHRVTIVE ANSRIGGRVE
110 120 130 140 150
TYRNKEEGWY ADLGAMRIPS DHSIFRWFAK TLGVKLNPFI MDDHNTFYFV
160 170 180 190 200
NGLLKRTYTV EANPDILNYK VRSSEKGKSA NTLFQDALQK VKDEVEAHGC
210 220 230 240 250
RAALMKYDKY SAKEYLKEVA GLSSEALRMI GDLLNEQSLM YTALSEMIYD
260 270 280 290 300
QADVNDNVQY DEVTGGTDLF PRAFLSVLDV PILLNSKVQR IRRSRDGVTV
310 320 330 340 350
SFKESQRSSL TDLHADMVLV TTTAKAALYM DFEPSLSIRK MEALRAVHYD
360 370 380 390 400
SSTKIILTFS SRFWEEDGIR GGKSITDRPS RYIYYPSHTF PANSSVGVLL
410 420 430 440 450
ASYTWSDDSL LLQAASDEEL KEMALRDLVK IHGERVRALC TGVVVKKWSL
460 470 480 490 500
DPYSFGAFAL FTPYQHLEYA KELFRSEGRV HFAGEHTAFP HAWMESAMKS
510 520
AIRAATNINK QTLLNEGMNE CPAPDEL
Length:527
Mass (Da):58,811
Last modified:October 19, 2011 - v2
Checksum:i856A038A55654C03
GO

Mass spectrometryi

Molecular mass is 61739.871 Da from positions 28 - 527. Determined by MALDI. 2 Publications
Molecular mass is 58810.79 Da from positions 28 - 527. Determined by MALDI. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ540313 mRNA. Translation: ADW77183.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HQ540313 mRNA. Translation: ADW77183.1.

3D structure databases

ProteinModelPortaliP86810.
SMRiP86810.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR002937. Amino_oxidase.
IPR023753. FAD/NAD-binding_dom.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiOXLA_SIGCA
AccessioniPrimary (citable) accession number: P86810
Secondary accession number(s): F8S6K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: October 19, 2011
Last modified: November 30, 2016
This is version 15 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.