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P86810

- OXLA_SIGCA

UniProt

P86810 - OXLA_SIGCA

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Protein
L-amino-acid oxidase
Gene
N/A
Organism
Siganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Inhibits the growth of both Gram-negative and Gram-positive bacteria. Displays strong antibacterial activity towards V.cholerae and E.tarda. Causes deformation of the surface of S.aureus and the formation of pores on the surface of E.coli. Strong antiparasitic activity is seen towards C.irritans, T.brucei and I.multifiliis. Cilia of treated theronts are lost and the macronucleus swells, inducing cell membrane rupture and efflux of the cytoplasm.2 Publications

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.By similarity

Cofactori

FAD By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981FAD By similarityBy similarity
Binding sitei117 – 1171Substrate By similarityBy similarity
Binding sitei288 – 2881FAD; via amide nitrogen and carbonyl oxygen By similarityBy similarity
Binding sitei403 – 4031Substrate By similarityBy similarity
Binding sitei485 – 4851FAD By similarityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi70 – 712FAD By similarityBy similarity
Nucleotide bindingi90 – 912FAD By similarityBy similarity
Nucleotide bindingi114 – 1174FAD By similarityBy similarity
Nucleotide bindingi492 – 4976FAD By similarityBy similarity
Nucleotide bindingi492 – 4932Substrate By similarityBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. defense response to bacterium Source: UniProtKB-KW
  2. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Oxidoreductase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
L-amino-acid oxidase (EC:1.4.3.2)
Alternative name(s):
Antiparasitic protein
Short name:
APP
Serum L-amino-acid oxidase
Short name:
SR-LAAO
OrganismiSiganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
Taxonomic identifieri75042 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataPercomorphariaSiganidaeSiganus

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed prediction1 Publication
Add
BLAST
Chaini28 – 527500L-amino-acid oxidase1 Publication
PRO_0000401107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi37 ↔ 200 By similarityBy similarity
Glycosylationi58 – 581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi393 – 3931N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expression mainly observed in plasma, spleen, kidney and gills with low levels detected in blood and no expression detected in brain, liver, heart, muscle or intestine (at protein level).2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP86810.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSiPR00757. AMINEOXDASEF.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P86810-1 [UniParc]FASTAAdd to Basket

« Hide

MDLHRAPWKS SAAAAVLLLA LFSGAAASSV EKNLAACLRD NDYDQLLQTV    50
QDGLPHINTS NHVVIVGAGV AGLTAAKLLQ DAGHRVTIVE ANSRIGGRVE 100
TYRNKEEGWY ADLGAMRIPS DHSIFRWFAK TLGVKLNPFI MDDHNTFYFV 150
NGLLKRTYTV EANPDILNYK VRSSEKGKSA NTLFQDALQK VKDEVEAHGC 200
RAALMKYDKY SAKEYLKEVA GLSSEALRMI GDLLNEQSLM YTALSEMIYD 250
QADVNDNVQY DEVTGGTDLF PRAFLSVLDV PILLNSKVQR IRRSRDGVTV 300
SFKESQRSSL TDLHADMVLV TTTAKAALYM DFEPSLSIRK MEALRAVHYD 350
SSTKIILTFS SRFWEEDGIR GGKSITDRPS RYIYYPSHTF PANSSVGVLL 400
ASYTWSDDSL LLQAASDEEL KEMALRDLVK IHGERVRALC TGVVVKKWSL 450
DPYSFGAFAL FTPYQHLEYA KELFRSEGRV HFAGEHTAFP HAWMESAMKS 500
AIRAATNINK QTLLNEGMNE CPAPDEL 527
Length:527
Mass (Da):58,811
Last modified:October 19, 2011 - v2
Checksum:i856A038A55654C03
GO

Mass spectrometryi

Molecular mass is 61739.871 Da from positions 28 - 527. Determined by MALDI. 1 Publication
Molecular mass is 58810.79 Da from positions 28 - 527. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HQ540313 mRNA. Translation: ADW77183.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HQ540313 mRNA. Translation: ADW77183.1 .

3D structure databases

ProteinModelPortali P86810.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
PRINTSi PR00757. AMINEOXDASEF.
ProtoNeti Search...

Publicationsi

  1. "The serum of rabbitfish (Siganus oramin) has antimicrobial activity to some pathogenic organisms and a novel serum L-amino acid oxidase is isolated."
    Wang F., Li R., Xie M., Li A.
    Fish Shellfish Immunol. 30:1095-1108(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-42; 98-118; 131-152; 209-218 AND 373-382, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Spleen.
  2. "A novel protein isolated from the serum of rabbitfish (Siganus oramin) is lethal to Cryptocaryon irritans."
    Wang F.H., Xie M.Q., Li A.X.
    Fish Shellfish Immunol. 29:32-41(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 28-42, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
    Tissue: Serum.

Entry informationi

Entry nameiOXLA_SIGCA
AccessioniPrimary (citable) accession number: P86810
Secondary accession number(s): F8S6K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: October 19, 2011
Last modified: June 11, 2014
This is version 9 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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