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P86810

- OXLA_SIGCA

UniProt

P86810 - OXLA_SIGCA

Protein

L-amino-acid oxidase

Gene
N/A
Organism
Siganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 10 (01 Oct 2014)
      Sequence version 2 (19 Oct 2011)
      Previous versions | rss
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    Functioni

    Inhibits the growth of both Gram-negative and Gram-positive bacteria. Displays strong antibacterial activity towards V.cholerae and E.tarda. Causes deformation of the surface of S.aureus and the formation of pores on the surface of E.coli. Strong antiparasitic activity is seen towards C.irritans, T.brucei and I.multifiliis. Cilia of treated theronts are lost and the macronucleus swells, inducing cell membrane rupture and efflux of the cytoplasm.2 Publications

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.By similarity

    Cofactori

    FAD.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei98 – 981FADBy similarity
    Binding sitei117 – 1171SubstrateBy similarity
    Binding sitei288 – 2881FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei403 – 4031SubstrateBy similarity
    Binding sitei485 – 4851FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi70 – 712FADBy similarity
    Nucleotide bindingi90 – 912FADBy similarity
    Nucleotide bindingi114 – 1174FADBy similarity
    Nucleotide bindingi492 – 4976FADBy similarity
    Nucleotide bindingi492 – 4932SubstrateBy similarity

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB-KW
    2. innate immune response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Oxidoreductase

    Keywords - Biological processi

    Immunity, Innate immunity

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase1 Publication (EC:1.4.3.2By similarity)
    Alternative name(s):
    Antiparasitic protein1 Publication
    Short name:
    APP1 Publication
    Serum L-amino-acid oxidase
    Short name:
    SR-LAAO1 Publication
    OrganismiSiganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
    Taxonomic identifieri75042 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataPercomorphariaSiganidaeSiganus

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 527500L-amino-acid oxidase1 PublicationPRO_0000401107Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi37 ↔ 200By similarity
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi393 – 3931N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expression mainly observed in plasma, spleen, kidney and gills with low levels detected in blood and no expression detected in brain, liver, heart, muscle or intestine (at protein level).2 Publications

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP86810.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family. FIG1 subfamily.Sequence Analysis

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00757. AMINEOXDASEF.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P86810-1 [UniParc]FASTAAdd to Basket

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    MDLHRAPWKS SAAAAVLLLA LFSGAAASSV EKNLAACLRD NDYDQLLQTV    50
    QDGLPHINTS NHVVIVGAGV AGLTAAKLLQ DAGHRVTIVE ANSRIGGRVE 100
    TYRNKEEGWY ADLGAMRIPS DHSIFRWFAK TLGVKLNPFI MDDHNTFYFV 150
    NGLLKRTYTV EANPDILNYK VRSSEKGKSA NTLFQDALQK VKDEVEAHGC 200
    RAALMKYDKY SAKEYLKEVA GLSSEALRMI GDLLNEQSLM YTALSEMIYD 250
    QADVNDNVQY DEVTGGTDLF PRAFLSVLDV PILLNSKVQR IRRSRDGVTV 300
    SFKESQRSSL TDLHADMVLV TTTAKAALYM DFEPSLSIRK MEALRAVHYD 350
    SSTKIILTFS SRFWEEDGIR GGKSITDRPS RYIYYPSHTF PANSSVGVLL 400
    ASYTWSDDSL LLQAASDEEL KEMALRDLVK IHGERVRALC TGVVVKKWSL 450
    DPYSFGAFAL FTPYQHLEYA KELFRSEGRV HFAGEHTAFP HAWMESAMKS 500
    AIRAATNINK QTLLNEGMNE CPAPDEL 527
    Length:527
    Mass (Da):58,811
    Last modified:October 19, 2011 - v2
    Checksum:i856A038A55654C03
    GO

    Mass spectrometryi

    Molecular mass is 61739.871 Da from positions 28 - 527. Determined by MALDI. 2 Publications
    Molecular mass is 58810.79 Da from positions 28 - 527. Determined by MALDI. 2 Publications

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HQ540313 mRNA. Translation: ADW77183.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HQ540313 mRNA. Translation: ADW77183.1 .

    3D structure databases

    ProteinModelPortali P86810.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    IPR001613. Flavin_amine_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00757. AMINEOXDASEF.
    ProtoNeti Search...

    Publicationsi

    1. "The serum of rabbitfish (Siganus oramin) has antimicrobial activity to some pathogenic organisms and a novel serum L-amino acid oxidase is isolated."
      Wang F., Li R., Xie M., Li A.
      Fish Shellfish Immunol. 30:1095-1108(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-42; 98-118; 131-152; 209-218 AND 373-382, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Tissue: Spleen1 Publication.
    2. "A novel protein isolated from the serum of rabbitfish (Siganus oramin) is lethal to Cryptocaryon irritans."
      Wang F.H., Xie M.Q., Li A.X.
      Fish Shellfish Immunol. 29:32-41(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 28-42, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Tissue: Serum1 Publication.

    Entry informationi

    Entry nameiOXLA_SIGCA
    AccessioniPrimary (citable) accession number: P86810
    Secondary accession number(s): F8S6K5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: October 19, 2011
    Last modified: October 1, 2014
    This is version 10 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3