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P86810 (OXLA_SIGCA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 18, 2012. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-amino-acid oxidase
EC=1.4.3.2
Alternative name(s):
Antiparasitic protein
Short name=APP
Serum L-amino-acid oxidase
Short name=SR-LAAO
OrganismSiganus canaliculatus (White-spotted spinefoot) (Chaetodon canaliculatus)
Taxonomic identifier75042 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Perciformes › Acanthuroidei › Siganidae › Siganus

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits the growth of both Gram-negative and Gram-positive bacteria. Displays strong antibacterial activity towards V.cholerae and E.tarda. Causes deformation of the surface of S.aureus and the formation of pores on the surface of E.coli. Strong antiparasitic activity is seen towards C.irritans, T.brucei and I.multifiliis. Cilia of treated theronts are lost and the macronucleus swells, inducing cell membrane rupture and efflux of the cytoplasm. Ref.1 Ref.2

Catalytic activity

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2. UniProtKB Q90W54

Cofactor

FAD By similarity. UniProtKB Q90W54

Subunit structure

Homodimer. Ref.2

Subcellular location

Secreted Ref.1 Ref.2.

Tissue specificity

Expression mainly observed in plasma, spleen, kidney and gills with low levels detected in blood and no expression detected in brain, liver, heart, muscle or intestine (at protein level). Ref.1 Ref.2

Sequence similarities

Belongs to the flavin monoamine oxidase family. FIG1 subfamily.

Mass spectrometry

Molecular mass is 61739.871 Da from positions 28 - 527. Determined by MALDI. Ref.2

Molecular mass is 58810.79 Da from positions 28 - 527. Determined by MALDI. Ref.1

Ontologies

Keywords
   Biological processImmunity
Innate immunity
   Cellular componentSecreted
   DomainSignal
   LigandFAD
Flavoprotein
   Molecular functionAntibiotic
Antimicrobial
Oxidoreductase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdefense response to bacterium

Inferred from electronic annotation. Source: UniProtKB-KW

innate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-amino-acid oxidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential Ref.2
Chain28 – 527500L-amino-acid oxidase Ref.1
PRO_0000401107

Regions

Nucleotide binding70 – 712FAD By similarity UniProtKB Q90W54
Nucleotide binding90 – 912FAD By similarity UniProtKB Q90W54
Nucleotide binding114 – 1174FAD By similarity UniProtKB Q90W54
Nucleotide binding492 – 4976FAD By similarity UniProtKB Q90W54
Nucleotide binding492 – 4932Substrate By similarity UniProtKB Q90W54

Sites

Binding site981FAD By similarity UniProtKB Q90W54
Binding site1171Substrate By similarity UniProtKB Q90W54
Binding site2881FAD; via amide nitrogen and carbonyl oxygen By similarity UniProtKB Q90W54
Binding site4031Substrate By similarity UniProtKB Q90W54
Binding site4851FAD By similarity UniProtKB Q90W54

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation3931N-linked (GlcNAc...) Potential
Disulfide bond37 ↔ 200 By similarity UniProtKB Q90W54

Sequences

Sequence LengthMass (Da)Tools
P86810 [UniParc].

Last modified October 19, 2011. Version 2.
Checksum: 856A038A55654C03

FASTA52758,811
        10         20         30         40         50         60 
MDLHRAPWKS SAAAAVLLLA LFSGAAASSV EKNLAACLRD NDYDQLLQTV QDGLPHINTS 

        70         80         90        100        110        120 
NHVVIVGAGV AGLTAAKLLQ DAGHRVTIVE ANSRIGGRVE TYRNKEEGWY ADLGAMRIPS 

       130        140        150        160        170        180 
DHSIFRWFAK TLGVKLNPFI MDDHNTFYFV NGLLKRTYTV EANPDILNYK VRSSEKGKSA 

       190        200        210        220        230        240 
NTLFQDALQK VKDEVEAHGC RAALMKYDKY SAKEYLKEVA GLSSEALRMI GDLLNEQSLM 

       250        260        270        280        290        300 
YTALSEMIYD QADVNDNVQY DEVTGGTDLF PRAFLSVLDV PILLNSKVQR IRRSRDGVTV 

       310        320        330        340        350        360 
SFKESQRSSL TDLHADMVLV TTTAKAALYM DFEPSLSIRK MEALRAVHYD SSTKIILTFS 

       370        380        390        400        410        420 
SRFWEEDGIR GGKSITDRPS RYIYYPSHTF PANSSVGVLL ASYTWSDDSL LLQAASDEEL 

       430        440        450        460        470        480 
KEMALRDLVK IHGERVRALC TGVVVKKWSL DPYSFGAFAL FTPYQHLEYA KELFRSEGRV 

       490        500        510        520 
HFAGEHTAFP HAWMESAMKS AIRAATNINK QTLLNEGMNE CPAPDEL

« Hide

References

[1]"The serum of rabbitfish (Siganus oramin) has antimicrobial activity to some pathogenic organisms and a novel serum L-amino acid oxidase is isolated."
Wang F., Li R., Xie M., Li A.
Fish Shellfish Immunol. 30:1095-1108(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-42; 98-118; 131-152; 209-218 AND 373-382, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
Tissue: Spleen.
[2]"A novel protein isolated from the serum of rabbitfish (Siganus oramin) is lethal to Cryptocaryon irritans."
Wang F.H., Xie M.Q., Li A.X.
Fish Shellfish Immunol. 29:32-41(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 28-42, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
Tissue: Serum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		HQ540313 mRNA. Translation: ADW77183.1.

3D structure databases

ProteinModelPortalP86810.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002937. Amino_oxidase.
IPR001613. Flavin_amine_oxidase.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
[Graphical view]
PRINTSPR00757. AMINEOXDASEF.
ProtoNetSearch...

Entry information

Entry nameOXLA_SIGCA
AccessionPrimary (citable) accession number: P86810
Secondary accession number(s): F8S6K5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: October 19, 2011
Last modified: April 18, 2012
This is version 7 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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