ID   CC21B_MOUSE             Reviewed;         133 AA.
AC   P86792; O09002; O09006; P84443; Q5M8M5; Q91V84;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   27-MAR-2024, entry version 87.
DE   RecName: Full=C-C motif chemokine 21b;
DE   AltName: Full=6Ckine;
DE   AltName: Full=Beta-chemokine exodus-2;
DE   AltName: Full=Small-inducible cytokine A21b;
DE   AltName: Full=Thymus-derived chemotactic agent 4;
DE            Short=TCA4;
DE   Flags: Precursor;
GN   Name=Ccl21b; Synonyms=Scya21, Scya21b;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Fetus;
RX   PubMed=9300671;
RA   Hromas R., Kim C.H., Klemsz M., Krathwohl M., Fife K., Cooper S.,
RA   Schnizlein-Bick C., Broxmeyer H.E.;
RT   "Isolation and characterization of Exodus-2, a novel C-C chemokine with a
RT   unique 37-amino acid carboxyl-terminal extension.";
RL   J. Immunol. 159:2554-2558(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/SvJ; TISSUE=Embryonic stem cell;
RX   PubMed=10523616; DOI=10.1084/jem.190.8.1183;
RA   Vassileva G., Soto H., Zlotnik A., Nakano H., Kakiuchi T., Hedrick J.A.,
RA   Lira S.A.;
RT   "The reduced expression of 6Ckine in the plt mouse results from the
RT   deletion of one of two 6Ckine genes.";
RL   J. Exp. Med. 190:1183-1188(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Ola;
RX   PubMed=11123313; DOI=10.4049/jimmunol.166.1.361;
RA   Nakano H., Gunn M.D.;
RT   "Gene duplications at the chemokine locus on mouse chromosome 4: multiple
RT   strain-specific haplotypes and the deletion of secondary lymphoid-organ
RT   chemokine and EBI-1 ligand chemokine genes in the plt mutation.";
RL   J. Immunol. 166:361-369(2001).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=9419363; DOI=10.1073/pnas.95.1.258;
RA   Gunn M.D., Tangemann K., Tam C., Cyster J.G., Rosen S.D., Williams L.T.;
RT   "A chemokine expressed in lymphoid high endothelial venules promotes the
RT   adhesion and chemotaxis of naive T lymphocytes.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:258-263(1998).
CC   -!- FUNCTION: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic
CC       in vitro for thymocytes and activated T-cells, but not for B-cells,
CC       macrophages, or neutrophils. Potent mesangial cell chemoattractant.
CC       Shows preferential activity towards naive T-cells. May play a role in
CC       mediating homing of lymphocytes to secondary lymphoid organs.
CC   -!- SUBUNIT: Binds to CCR7 and to CXCR3. Interacts with PDPN; relocalizes
CC       PDPN to the basolateral membrane. Interacts with GPR174.
CC       {ECO:0000250|UniProtKB:O00585, ECO:0000250|UniProtKB:P84444}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed strongly in lung, spleen, thymus,
CC       peripheral and mesentric lymph nodes. Also expressed in the testis,
CC       kidney, liver, and heart.
CC   -!- MISCELLANEOUS: Three genes code for Ccl21 in mouse. Ccl21b and Ccl21c
CC       produce identical proteins while the protein produced by Ccl21a differs
CC       at only one position. Ccl21b and Ccl21c have Leu-65 (6Ckine-Leu) while
CC       Ccl21a has 'Ser-65' (6Ckine-Ser).
CC   -!- SIMILARITY: Belongs to the intercrine beta (chemokine CC) family.
CC       {ECO:0000305}.
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DR   EMBL; U88322; AAC17929.1; -; mRNA.
DR   EMBL; AF171085; AAF16900.1; -; Genomic_DNA.
DR   EMBL; AF307986; AAG45834.1; -; Genomic_DNA.
DR   EMBL; AL824709; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS51145.1; -.
DR   RefSeq; NP_001180595.1; NM_001193666.1.
DR   RefSeq; NP_001180597.1; NM_001193668.1.
DR   RefSeq; NP_001257289.1; NM_001270360.1.
DR   RefSeq; NP_035465.2; NM_011335.2.
DR   RefSeq; NP_075539.1; NM_023052.2.
DR   RefSeq; XP_001473308.1; XM_001473258.5.
DR   RefSeq; XP_001473444.1; XM_001473394.6.
DR   AlphaFoldDB; P86792; -.
DR   SMR; P86792; -.
DR   STRING; 10090.ENSMUSP00000095727; -.
DR   jPOST; P86792; -.
DR   PaxDb; 10090-ENSMUSP00000095727; -.
DR   PeptideAtlas; P86792; -.
DR   ProteomicsDB; 279943; -.
DR   DNASU; 65956; -.
DR   Ensembl; ENSMUST00000098123.4; ENSMUSP00000095727.4; ENSMUSG00000073878.4.
DR   Ensembl; ENSMUST00000098128.4; ENSMUSP00000095732.4; ENSMUSG00000094065.2.
DR   Ensembl; ENSMUST00000178168.3; ENSMUSP00000136903.2; ENSMUSG00000096596.3.
DR   Ensembl; ENSMUST00000178864.3; ENSMUSP00000137149.2; ENSMUSG00000095675.3.
DR   GeneID; 100042493; -.
DR   GeneID; 100504239; -.
DR   GeneID; 100504346; -.
DR   GeneID; 100862177; -.
DR   KEGG; mmu:100042493; -.
DR   KEGG; mmu:100504239; -.
DR   KEGG; mmu:100504346; -.
DR   KEGG; mmu:100862177; -.
DR   UCSC; uc008skt.1; mouse.
DR   AGR; MGI:1349182; -.
DR   CTD; 100042493; -.
DR   CTD; 100862177; -.
DR   MGI; MGI:1349182; Ccl21b.
DR   VEuPathDB; HostDB:ENSMUSG00000073878; -.
DR   VEuPathDB; HostDB:ENSMUSG00000094065; -.
DR   VEuPathDB; HostDB:ENSMUSG00000094121; -.
DR   VEuPathDB; HostDB:ENSMUSG00000095675; -.
DR   VEuPathDB; HostDB:ENSMUSG00000096271; -.
DR   VEuPathDB; HostDB:ENSMUSG00000096596; -.
DR   VEuPathDB; HostDB:ENSMUSG00000096873; -.
DR   eggNOG; ENOG502S8D1; Eukaryota.
DR   HOGENOM; CLU_141716_3_2_1; -.
DR   InParanoid; P86792; -.
DR   OMA; SDCCLKH; -.
DR   OrthoDB; 5265378at2759; -.
DR   PhylomeDB; P86792; -.
DR   Reactome; R-MMU-380108; Chemokine receptors bind chemokines.
DR   Reactome; R-MMU-418594; G alpha (i) signalling events.
DR   BioGRID-ORCS; 100042493; 4 hits in 37 CRISPR screens.
DR   BioGRID-ORCS; 100504239; 0 hits in 11 CRISPR screens.
DR   BioGRID-ORCS; 100504346; 0 hits in 12 CRISPR screens.
DR   BioGRID-ORCS; 100862177; 2 hits in 23 CRISPR screens.
DR   BioGRID-ORCS; 65956; 0 hits in 4 CRISPR screens.
DR   ChiTaRS; Ccl21a; mouse.
DR   PRO; PR:P86792; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; P86792; Protein.
DR   Bgee; ENSMUSG00000073878; Expressed in urinary bladder and 29 other cell types or tissues.
DR   ExpressionAtlas; P86792; baseline and differential.
DR   GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0048020; F:CCR chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0008009; F:chemokine activity; ISO:MGI.
DR   GO; GO:0042379; F:chemokine receptor binding; ISO:MGI.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:MGI.
DR   GO; GO:0060326; P:cell chemotaxis; ISO:MGI.
DR   GO; GO:0071347; P:cellular response to interleukin-1; IBA:GO_Central.
DR   GO; GO:0071356; P:cellular response to tumor necrosis factor; IBA:GO_Central.
DR   GO; GO:0071346; P:cellular response to type II interferon; IBA:GO_Central.
DR   GO; GO:0070098; P:chemokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0002407; P:dendritic cell chemotaxis; ISO:MGI.
DR   GO; GO:0001768; P:establishment of T cell polarity; ISO:MGI.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISO:MGI.
DR   GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR   GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR   GO; GO:0030595; P:leukocyte chemotaxis; ISO:MGI.
DR   GO; GO:0048247; P:lymphocyte chemotaxis; IBA:GO_Central.
DR   GO; GO:0035759; P:mesangial cell-matrix adhesion; ISO:MGI.
DR   GO; GO:0002548; P:monocyte chemotaxis; IBA:GO_Central.
DR   GO; GO:2000669; P:negative regulation of dendritic cell apoptotic process; ISO:MGI.
DR   GO; GO:1903237; P:negative regulation of leukocyte tethering or rolling; ISO:MGI.
DR   GO; GO:0045638; P:negative regulation of myeloid cell differentiation; ISO:MGI.
DR   GO; GO:0030593; P:neutrophil chemotaxis; IBA:GO_Central.
DR   GO; GO:0030838; P:positive regulation of actin filament polymerization; ISO:MGI.
DR   GO; GO:0043123; P:positive regulation of canonical NF-kappaB signal transduction; ISO:MGI.
DR   GO; GO:0033630; P:positive regulation of cell adhesion mediated by integrin; ISO:MGI.
DR   GO; GO:2000147; P:positive regulation of cell motility; ISO:MGI.
DR   GO; GO:0001954; P:positive regulation of cell-matrix adhesion; ISO:MGI.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:MGI.
DR   GO; GO:0051491; P:positive regulation of filopodium assembly; ISO:MGI.
DR   GO; GO:0046330; P:positive regulation of JNK cascade; ISO:MGI.
DR   GO; GO:2000529; P:positive regulation of myeloid dendritic cell chemotaxis; ISO:MGI.
DR   GO; GO:0090023; P:positive regulation of neutrophil chemotaxis; ISO:MGI.
DR   GO; GO:0051897; P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; ISO:MGI.
DR   GO; GO:0031274; P:positive regulation of pseudopodium assembly; ISO:MGI.
DR   GO; GO:0051209; P:release of sequestered calcium ion into cytosol; ISO:MGI.
DR   GO; GO:0034695; P:response to prostaglandin E; ISO:MGI.
DR   GO; GO:0031529; P:ruffle organization; ISO:MGI.
DR   Gene3D; 2.40.50.40; -; 1.
DR   InterPro; IPR039809; Chemokine_b/g/d.
DR   InterPro; IPR001811; Chemokine_IL8-like_dom.
DR   InterPro; IPR036048; Interleukin_8-like_sf.
DR   PANTHER; PTHR12015:SF72; C-C MOTIF CHEMOKINE 21; 1.
DR   PANTHER; PTHR12015; SMALL INDUCIBLE CYTOKINE A; 1.
DR   Pfam; PF00048; IL8; 1.
DR   SMART; SM00199; SCY; 1.
DR   SUPFAM; SSF54117; Interleukin 8-like chemokines; 1.
DR   Genevisible; P86792; MM.
PE   1: Evidence at protein level;
KW   Chemotaxis; Cytokine; Disulfide bond; Inflammatory response;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..133
FT                   /note="C-C motif chemokine 21b"
FT                   /id="PRO_0000005222"
FT   REGION          87..133
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          98..133
FT                   /note="C-terminal basic extension"
FT   DISULFID        31..57
FT                   /evidence="ECO:0000250"
FT   DISULFID        32..75
FT                   /evidence="ECO:0000250"
FT   DISULFID        103..122
FT                   /evidence="ECO:0000255"
FT   CONFLICT        13
FT                   /note="V -> D (in Ref. 1; AAC17929)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   133 AA;  14584 MW;  7071750A2089A70B CRC64;
     MAQMMTLSLL SLVLALCIPW TQGSDGGGQD CCLKYSQKKI PYSIVRGYRK QEPSLGCPIP
     AILFLPRKHS KPELCANPEE GWVQNLMRRL DQPPAPGKQS PGCRKNRGTS KSGKKGKGSK
     GCKRTEQTQP SRG
//