P86687 (HUGA_POLPI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hyaluronidase Short name=Hya EC=3.2.1.35 Alternative name(s): Hyaluronoglucosaminidase
Organism	Polybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp)
Taxonomic identifier	291283 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Polistinae › Epiponini › Polybia

Protein attributes

Sequence length	288 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Allergenic properties	Causes an allergic reaction in human. Ref.1
Miscellaneous	On the 2D-gel the determined pI of this protein is: 9.5, its MW is: 44.1 kDa. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Allergen
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›288	›288	Hyaluronidase		PRO_0000396653
Sites
	Active site	66	1	Proton donor By similarity UniProtKB Q08169
Amino acid modifications
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Glycosylation	282	1	N-linked (GlcNAc...) Potential
	Disulfide bond	142 ↔ 154		By similarity UniProtKB Q08169
	Disulfide bond	? ↔ 265		By similarity UniProtKB Q08169
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	288	1

Sequences

	Sequence		Length	Mass (Da)
	P86687 [UniParc]. Last modified August 10, 2010. Version 1. Checksum: ABFF0679F329016F	FASTA	288	33,746
	10 20 30 40 50 60 GETISIYYDP GKFPALMPLK NGNYEERNGG VPQRGNITIH LQQFNEDLDK MTPDKNFGGI 70 80 90 100 110 120 GVIDFERWKP IFRQNWGNTE IHKKYSIELV RYEHPKWSES MIEAEATKKF EKYARLFMEE 130 140 150 160 170 180 TLKLAKKTRK RAKWGYYGFP YCYNYTPNNP GPDCDAKAMI ENDRLSWMYN NQEILFPSVY 190 200 210 220 230 240 VRHELTPDQR VYLVQGRIKE AVRISNNLKH SPKVLSYWWY VYQDKMDIFL SETDVKKTFQ 250 260 270 280 EIVTNGGDGI IIWGSSSDVN SLSKCKRLRE YLLNTLGPFA VNVTETVN

References

[1]	"Molecular characterization of Hyaluronidase allergen from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)." Pinto J.R.A.S. Thesis (2008), Sao Paulo State University, Brazil Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN. Tissue: Venom.
[2]	"Structural characterization of hyaluronidase isoforms from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)." Pinto J.R.A.S., Santos L.D., Arcuri H.A., Dias N.B., Palma M.S. Submitted (JUN-2010) to UniProtKB Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001329. Glyco_hydro_56_Api/Dol. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PRINTS	PR00846. GLHYDRLASE56. PR00847. HYALURONDASE.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUGA_POLPI

Accession

Primary (citable) accession number: P86687

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	August 10, 2010
Last modified:	March 6, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)