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Protein

Hyaluronidase

Gene
N/A
Organism
Polybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei66 – 661Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase2 Publications (EC:3.2.1.35)
Short name:
HyaBy similarity
Alternative name(s):
HyaluronoglucosaminidaseBy similarity
OrganismiPolybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp)
Taxonomic identifieri291283 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaePolistinaeEpiponiniPolybia

Subcellular locationi

  1. Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›288›288HyaluronidasePRO_0000396653Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi? ↔ 265By similarity
Glycosylationi36 – 361N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 154By similarity
Glycosylationi282 – 2821N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Fragment.

P86687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GETISIYYDP GKFPALMPLK NGNYEERNGG VPQRGNITIH LQQFNEDLDK
60 70 80 90 100
MTPDKNFGGI GVIDFERWKP IFRQNWGNTE IHKKYSIELV RYEHPKWSES
110 120 130 140 150
MIEAEATKKF EKYARLFMEE TLKLAKKTRK RAKWGYYGFP YCYNYTPNNP
160 170 180 190 200
GPDCDAKAMI ENDRLSWMYN NQEILFPSVY VRHELTPDQR VYLVQGRIKE
210 220 230 240 250
AVRISNNLKH SPKVLSYWWY VYQDKMDIFL SETDVKKTFQ EIVTNGGDGI
260 270 280
IIWGSSSDVN SLSKCKRLRE YLLNTLGPFA VNVTETVN
Length:288
Mass (Da):33,746
Last modified:August 10, 2010 - v1
Checksum:iABFF0679F329016F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Curated
Non-terminal residuei288 – 2881Curated

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of Hyaluronidase allergen from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)."
    Pinto J.R.A.S.
    Thesis (2008), Sao Paulo State University, Brazil
    Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Venom1 Publication.
  2. "Structural characterization of hyaluronidase isoforms from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)."
    Pinto J.R.A.S., Santos L.D., Arcuri H.A., Dias N.B., Palma M.S.
    Submitted (JUN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE.
    Tissue: Venom1 Publication.

Entry informationi

Entry nameiHUGA_POLPI
AccessioniPrimary (citable) accession number: P86687
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: April 1, 2015
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 9.5, its MW is: 44.1 kDa.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.