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P86687 (HUGA_POLPI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronidase

Short name=Hya
EC=3.2.1.35
Alternative name(s):
Hyaluronoglucosaminidase
OrganismPolybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp)
Taxonomic identifier291283 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaePolistinaeEpiponiniPolybia

Protein attributes

Sequence length288 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity.

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.1

Subcellular location

Secreted Ref.1.

Tissue specificity

Expressed by the venom gland. Ref.1

Allergenic properties

Causes an allergic reaction in human. Ref.1

Miscellaneous

On the 2D-gel the determined pI of this protein is: 9.5, its MW is: 44.1 kDa. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseAllergen
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhyalurononglucosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›288›288Hyaluronidase
PRO_0000396653

Sites

Active site661Proton donor By similarity UniProtKB Q08169

Amino acid modifications

Glycosylation361N-linked (GlcNAc...) Potential
Glycosylation2821N-linked (GlcNAc...) Potential
Disulfide bond142 ↔ 154 By similarity UniProtKB Q08169
Disulfide bond? ↔ 265 By similarity UniProtKB Q08169

Experimental info

Non-terminal residue11
Non-terminal residue2881

Sequences

Sequence LengthMass (Da)Tools
P86687 [UniParc].

Last modified August 10, 2010. Version 1.
Checksum: ABFF0679F329016F

FASTA28833,746
        10         20         30         40         50         60 
GETISIYYDP GKFPALMPLK NGNYEERNGG VPQRGNITIH LQQFNEDLDK MTPDKNFGGI 

        70         80         90        100        110        120 
GVIDFERWKP IFRQNWGNTE IHKKYSIELV RYEHPKWSES MIEAEATKKF EKYARLFMEE 

       130        140        150        160        170        180 
TLKLAKKTRK RAKWGYYGFP YCYNYTPNNP GPDCDAKAMI ENDRLSWMYN NQEILFPSVY 

       190        200        210        220        230        240 
VRHELTPDQR VYLVQGRIKE AVRISNNLKH SPKVLSYWWY VYQDKMDIFL SETDVKKTFQ 

       250        260        270        280 
EIVTNGGDGI IIWGSSSDVN SLSKCKRLRE YLLNTLGPFA VNVTETVN 

« Hide

References

[1]"Molecular characterization of Hyaluronidase allergen from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)."
Pinto J.R.A.S.
Thesis (2008), Sao Paulo State University, Brazil
Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
Tissue: Venom.
[2]"Structural characterization of hyaluronidase isoforms from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae)."
Pinto J.R.A.S., Santos L.D., Arcuri H.A., Dias N.B., Palma M.S.
Submitted (JUN-2010) to UniProtKB
Cited for: PROTEIN SEQUENCE.
Tissue: Venom.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001329. Venom_Hyaluronidase.
[Graphical view]
PANTHERPTHR11769. PTHR11769. 1 hit.
PfamPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PRINTSPR00846. GLHYDRLASE56.
PR00847. HYALURONDASE.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHUGA_POLPI
AccessionPrimary (citable) accession number: P86687
Entry history
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: August 10, 2010
Last modified: December 11, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries