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P86682 (HETN_HAEDE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 6. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Hementerin

EC=3.4.-.-
OrganismHaementeria depressa (Leech)
Taxonomic identifier279730 [NCBI]
Taxonomic lineageEukaryotaMetazoaLophotrochozoaAnnelidaClitellataHirudinidaHirudineaRhynchobdellidaGlossiphoniidaeHaementeria

Protein attributes

Sequence length30 AA.
Sequence statusFragments.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves fibrinogen Aalpha (FGA), gamma (FGG) and Bbeta (FGB) chains. Degrades cross-linked fibrin. Has no amidolytic, plasminogenolytic or caseinolytic activity. Inhibits platelet aggregation induced by collagen (IC50=7.5ug/ml) and various other agonists, presumably via activation of a nitridergic pathway. Inhibition is accompanied by reduced ATP release from and surface expression of SELP and CD63 on platelets as well as increased intracellular levels of Ca2+, cGMP and nitric oxide synthase activity. Ref.1 Ref.2

Cofactor

Calcium. Ref.1

Enzyme regulation

Fibrino(geno)lytic activity inhibited by EDTA but not by PMSF, E-64, 6-AHA and aprotinin. Ref.1

Subcellular location

Secreted Probable.

Caution

The order of the peptides shown is unknown. Ref.1

Ontologies

Keywords
   Biological processBlood coagulation
Fibrinolysis
Hemostasis
   Cellular componentSecreted
   LigandCalcium
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processblood coagulation

Inferred from electronic annotation. Source: UniProtKB-KW

fibrinolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetallopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›30›30Hementerin
PRO_0000397938

Experimental info

Non-adjacent residues9 – 102
Non-adjacent residues16 – 172
Non-terminal residue301

Sequences

Sequence LengthMass (Da)Tools
P86682 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 5A492FA070690DD1

FASTA303,513
        10         20         30 
XTLSEPEPTC SIEYFRYQAI EDCEYSISVK 

« Hide

References

[1]"Fibrino(geno)lytic properties of purified hementerin, a metalloproteinase from the leech Haementeria depressa."
Chudzinski-Tavassi A.M., Kelen E.M., de Paula Rosa A.P., Loyau S., Sampaio C.A., Bon C., Angles-Cano E.
Thromb. Haemost. 80:155-160(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, COFACTOR, ENZYME REGULATION.
Tissue: Salivary gland.
[2]"Nitridergic platelet pathway activation by hementerin, a metalloprotease from the leech Haementeria depressa."
Chudzinski-Tavassi A.M., Bermej E., Rosenstein R.E., Faria F., Sarmiento M.I., Alberto F., Sampaio M.U., Lazzari M.A.
Biol. Chem. 384:1333-1339(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameHETN_HAEDE
AccessionPrimary (citable) accession number: P86682
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: May 14, 2014
This is version 6 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)