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P86535

- OXLA1_DABRR

UniProt

P86535 - OXLA1_DABRR

Protein

L-amino-acid oxidase L1

Gene
N/A
Organism
Daboia russelii (Russel's viper) (Vipera russelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions By similarity.By similarity

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.By similarity

    Cofactori

    FAD.1 Publication

    Kineticsi

    1. KM=0.297 mM for L-Met1 Publication
    2. KM=1.44 mM for L-Ile1 Publication
    3. KM=0.750 mM for L-Leu1 Publication
    4. KM=0.066 mM for L-Phe1 Publication
    5. KM=0.210 mM for L-Trp1 Publication
    6. KM=0.052 mM for L-Tyr1 Publication

    Vmax=8.96 µmol/min/mg enzyme toward L-Phe1 Publication

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. hemolysis in other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Toxin

    Keywords - Biological processi

    Apoptosis, Cytolysis, Hemolysis

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase L1 (EC:1.4.3.2)
    Short name:
    LAAOBy similarity
    Short name:
    LAAO-L1
    Short name:
    LAO1 Publication
    OrganismiDaboia russelii (Russel's viper) (Vipera russelii)
    Taxonomic identifieri31159 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›20›20L-amino-acid oxidase L1PRO_0000394725Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi10 ↔ ?By similarity

    Post-translational modificationi

    N-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.2 Publications

    Interactioni

    Subunit structurei

    Monomer Probable. This is in contrast with most of its orthologs, that are non-covalently linked homodimers.1 PublicationCurated

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the flavin monoamine oxidase family. FIG1 subfamily.Sequence Analysis

    Sequencei

    Sequence statusi: Fragment.

    P86535-1 [UniParc]FASTAAdd to Basket

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    ADDINPKEEC FFEDDYYEFE                                    20
    Length:20
    Mass (Da):2,519
    Last modified:September 21, 2011 - v2
    Checksum:i999C77D7C86348E7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 41I → K AA sequence (PubMed:20203422)Curated
    Sequence conflicti7 – 71K → L AA sequence (PubMed:20203422)Curated
    Sequence conflicti12 – 121F → R AA sequence (PubMed:20203422)Curated
    Sequence conflicti17 – 171Y → E AA sequence (PubMed:20203422)Curated
    Non-terminal residuei20 – 201

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Two L-amino acid oxidase isoenzymes from Russell's viper (Daboia russelli russelli) venom with different mechanisms of inhibition by substrate analogs."
      Mandal S., Bhattacharyya D.
      FEBS J. 275:2078-2095(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INHIBITION BY SUBSTRATE ANALOGS.
      Tissue: Venom.
    2. "Molecular diversity in venom proteins of the Russell's viper (Daboia russellii russellii) and the Indian cobra (Naja naja) in Sri Lanka."
      Suzuki M., Itoh T., Bandaranayake B.M.A.I.K., Ranasinghe J.G., Athauda S.B., Moriyama A.
      Biomed. Res. 31:71-81(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-19, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Venom1 Publication.

    Entry informationi

    Entry nameiOXLA1_DABRR
    AccessioniPrimary (citable) accession number: P86535
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 16 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3