P86450 (XYL1_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-xylosidase/alpha-L-arabinofuranosidase 1

Alternative name(s):
Xylan 1,4-beta-xylosidase/Alpha-N-arabinofuranosidase 1

Including the following 2 domains:

1. Beta-xylosidase
   EC=3.2.1.37
   Alternative name(s):
   1,4-beta-D-xylan xylohydrolase
   Xylan 1,4-beta-xylosidase
2. Alpha-N-arabinofuranosidase
   EC=3.2.1.55
   Alternative name(s):
   Alpha-L-arabinofuranosidase
   Short name=Arabinosidase

Gene names

Name:

Xyl1

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	45 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	A bifunctional beta-xylosidase/alpha-L-arabinosidase, exo-enzyme that acts synergistically with endohydrolases. Releases xylose and arabinose from cell walls By similarity. UniProtKB A5JTQ2
Catalytic activity	Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini. UniProtKB A5JTQ2 Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides. UniProtKB A5JTQ2
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity UniProtKB Q9FLG1.
Tissue specificity	Strongly expressed in young roots, significantly reduced expression in older roots. Highest expression levels seen in root tips, some expression seen in root nodules and in the flowers, but not seen in other aerial parts of the plant such as in the stems, hypocotyls or leaves. Ref.1
Sequence similarities	Belongs to the glycoside hydrolase 3 family.

Ontologies

Keywords
Biological process	Xylan degradation
Cellular component	Extracellular matrix Secreted
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing Multifunctional enzyme
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC xylan 1,4-beta-xylosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›45	›45	Beta-xylosidase/alpha-L-arabinofuranosidase 1		PRO_0000392639
Experimental info
	Non-adjacent residues	15 – 16	2
	Non-adjacent residues	30 – 31	2
	Non-terminal residue	1	1
	Non-terminal residue	45	1

Sequences

	Sequence		Length	Mass (Da)
	P86450 [UniParc]. Last modified March 23, 2010. Version 1. Checksum: 83339885B81AEA45	FASTA	45	4,785
	10 20 30 40 GVQRYTFDAV VSQQDTILSG LDLDCGSYLG YTSPLQGLTA FVPTS

References

[1]

"Molecular cloning of a bifunctional beta-xylosidase/alpha-L-arabinosidase from alfalfa roots: heterologous expression in Medicago truncatula and substrate specificity of the purified enzyme." Xiong J.S., Balland-Vanney M., Xie Z.P., Schultze M., Kondorosi A., Kondorosi E., Staehelin C. J. Exp. Bot. 58:2799-2810(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, TISSUE SPECIFICITY. Strain: cv. Sitel. Tissue: Root nodule.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYL1_MEDSA

Accession

Primary (citable) accession number: P86450

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	March 23, 2010
Last modified:	June 15, 2010
This is version 3 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program