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Galerina sp.
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli


Lignin degradation and detoxification of lignin-derived products. Demethylates eucalyptus hard wood lignin. Has high activity against the non-phenolic heterocyclic compound ABTS, and lower activity against the phenolic substrates syringic acid, caffeic acid, syringaldazine, vanillic acid, catechol and levodihydroxyphenylalanine.1 Publication


On the 2D-gel the determined pI of this protein is: 4, its MW is: 64 kDa.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication


Cu cationBy similarity1 PublicationNote: Binds 4 Cu cations per monomer.By similarity1 Publication

Enzyme regulationi

Strongly inhibited by sodium azide, sodium cyanide, Li+, Sn+, Hg2+, and the disulfide-reducing agents beta-mercaptoethanol, dithiothreitol and thioglycolic acid. Moderately inhibited by Mn2+ and Fe2+, inhibition by these metal ions is stronger at 0.1 mM than at 1 mM. Moderately inhibited by Cu2+.1 Publication


  1. KM=14 µM for ABTS1 Publication
  2. KM=0.8 µM for syringaldazine1 Publication

    pH dependencei

    Optimum pH is 3.0 with ABTS as substrate, activity decreases sharply as pH increases, and no activity is seen at pH 7.0. Optimum pH is 4.0 with syringic acid as substrate, 5.0 with caffeic acid, syringaldazine, vanillic acid or catechol as substrate, and 6.0 with levodihydroxyphenylalanine as substrate. Inactive after 3 hours incubation at pH 2.0, retains 20% of its activity after 24 hours at pH 3.0 and 90% of its activity after 24 hours at pH 9.0.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius. Shows 50% of its maximal activity at 20 degrees Celsius. Has a half-life of 53 minutes at 50 degrees Celsius, 23 minutes at 60 degrees Celsius, and less than 2 minutes at 70 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi


    Molecular functionOxidoreductase
    Biological processLignin degradation
    LigandCopper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-11 PublicationBy similarity (EC:
    Short name:
    Lac11 Publication
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase 1By similarity
    Diphenol oxidase 1By similarity
    Urishiol oxidase 1By similarity
    OrganismiGalerina sp.
    Taxonomic identifieri671179 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesStrophariaceaeGalerina

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Keywords - Cellular componenti


    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000399056‹1 – ›23Laccase-1Add BLAST›23

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Sequence analysis


    Sequence statusi: Fragments.

    P86351-1 [UniParc]FASTAAdd to basket

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            10         20 
    Mass (Da):2,303
    Last modified:October 5, 2010 - v1

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Non-terminal residuei11 Publication1
    Non-adjacent residuesi11 – 121 Publication2
    Non-terminal residuei231 Publication1

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiLAC1_GALSP
    AccessioniPrimary (citable) accession number: P86351
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: January 7, 2015
    This is version 14 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program


    Keywords - Technical termi

    Direct protein sequencing


    1. SIMILARITY comments
      Index of protein domains and families