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P86351

- LAC1_GALSP

UniProt

P86351 - LAC1_GALSP

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Protein

Laccase-1

Gene
N/A
Organism
Galerina sp.
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products. Demethylates eucalyptus hard wood lignin. Has high activity against the non-phenolic heterocyclic compound ABTS, and lower activity against the phenolic substrates syringic acid, caffeic acid, syringaldazine, vanillic acid, catechol and levodihydroxyphenylalanine.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Binds 4 copper ions per monomer.By similarity1 Publication

Enzyme regulationi

Strongly inhibited by sodium azide, sodium cyanide, Li+, Sn+, Hg2+, and the disulfide-reducing agents beta-mercaptoethanol, dithiothreitol and thioglycolic acid. Moderately inhibited by Mn2+ and Fe2+, inhibition by these metal ions is stronger at 0.1 mM than at 1 mM. Moderately inhibited by Cu2+.1 Publication

Kineticsi

  1. KM=14 µM for ABTS1 Publication
  2. KM=0.8 µM for syringaldazine1 Publication

pH dependencei

Optimum pH is 3.0 with ABTS as substrate, activity decreases sharply as pH increases, and no activity is seen at pH 7.0. Optimum pH is 4.0 with syringic acid as substrate, 5.0 with caffeic acid, syringaldazine, vanillic acid or catechol as substrate, and 6.0 with levodihydroxyphenylalanine as substrate. Inactive after 3 hours incubation at pH 2.0, retains 20% of its activity after 24 hours at pH 3.0 and 90% of its activity after 24 hours at pH 9.0.1 Publication

Temperature dependencei

Optimum temperature is 60 degrees Celsius. Shows 50% of its maximal activity at 20 degrees Celsius. Has a half-life of 53 minutes at 50 degrees Celsius, 23 minutes at 60 degrees Celsius, and less than 2 minutes at 70 degrees Celsius.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-1 (EC:1.10.3.2)
Short name:
Lac1
Alternative name(s):
Benzenediol:oxygen oxidoreductase 1
Diphenol oxidase 1
Urishiol oxidase 1
OrganismiGalerina sp.
Taxonomic identifieri671179 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesStrophariaceaeGalerina

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›23›23Laccase-1PRO_0000399056Add
BLAST

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragments.

P86351-1 [UniParc]FASTAAdd to Basket

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GPSTDLVIGN KGFDGGIDSA ILR                                23
Length:23
Mass (Da):2,303
Last modified:October 5, 2010 - v1
Checksum:i1CF71949A9EC8945
GO

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi11 – 122

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei23 – 231

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Blue laccase from Galerina sp.: properties and potential for Kraft lignin demethylation."
    Ibrahim V., Mendoza L., Mamo G., Hatti-Kaul R.
    Process Biochem. 46:379-384(2010)
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: HC1 / DSM 22662.

Entry informationi

Entry nameiLAC1_GALSP
AccessioniPrimary (citable) accession number: P86351
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: February 19, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 4, its MW is: 64 kDa.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3