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Protein

Laccase-1

Gene
N/A
Organism
Galerina sp.
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products. Demethylates eucalyptus hard wood lignin. Has high activity against the non-phenolic heterocyclic compound ABTS, and lower activity against the phenolic substrates syringic acid, caffeic acid, syringaldazine, vanillic acid, catechol and levodihydroxyphenylalanine.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu cationBy similarity1 PublicationNote: Binds 4 Cu cations per monomer.By similarity1 Publication

Enzyme regulationi

Strongly inhibited by sodium azide, sodium cyanide, Li+, Sn+, Hg2+, and the disulfide-reducing agents beta-mercaptoethanol, dithiothreitol and thioglycolic acid. Moderately inhibited by Mn2+ and Fe2+, inhibition by these metal ions is stronger at 0.1 mM than at 1 mM. Moderately inhibited by Cu2+.1 Publication

Kineticsi

  1. KM=14 µM for ABTS1 Publication
  2. KM=0.8 µM for syringaldazine1 Publication

    pH dependencei

    Optimum pH is 3.0 with ABTS as substrate, activity decreases sharply as pH increases, and no activity is seen at pH 7.0. Optimum pH is 4.0 with syringic acid as substrate, 5.0 with caffeic acid, syringaldazine, vanillic acid or catechol as substrate, and 6.0 with levodihydroxyphenylalanine as substrate. Inactive after 3 hours incubation at pH 2.0, retains 20% of its activity after 24 hours at pH 3.0 and 90% of its activity after 24 hours at pH 9.0.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius. Shows 50% of its maximal activity at 20 degrees Celsius. Has a half-life of 53 minutes at 50 degrees Celsius, 23 minutes at 60 degrees Celsius, and less than 2 minutes at 70 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-11 PublicationBy similarity (EC:1.10.3.2)
    Short name:
    Lac11 Publication
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase 1By similarity
    Diphenol oxidase 1By similarity
    Urishiol oxidase 1By similarity
    OrganismiGalerina sp.
    Taxonomic identifieri671179 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesStrophariaceaeGalerina

    Subcellular locationi

    • Secreted 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›23›23Laccase-1PRO_0000399056Add
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Sequence Analysis

    Sequencei

    Sequence statusi: Fragments.

    P86351-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20 
    GPSTDLVIGN KGFDGGIDSA ILR
    Length:23
    Mass (Da):2,303
    Last modified:October 5, 2010 - v1
    Checksum:i1CF71949A9EC8945
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 111 Publication
    Non-adjacent residuesi11 – 1221 Publication
    Non-terminal residuei23 – 2311 Publication

    Cross-referencesi

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    ProtoNetiSearch...

    Publicationsi

    1. "Blue laccase from Galerina sp.: properties and potential for Kraft lignin demethylation."
      Ibrahim V., Mendoza L., Mamo G., Hatti-Kaul R.
      Process Biochem. 46:379-384(2010)
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
      Strain: HC1 / DSM 226621 Publication.

    Entry informationi

    Entry nameiLAC1_GALSP
    AccessioniPrimary (citable) accession number: P86351
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 5, 2010
    Last modified: January 7, 2015
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    On the 2D-gel the determined pI of this protein is: 4, its MW is: 64 kDa.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.