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Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli


Lignin degradation and detoxification of lignin-derived products (Probable). Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.Curated1 Publication


On the 2D-gel the determined pI of this protein is: 4.05, its MW is: 75 kDa.

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication


Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Enzyme regulationi

Inhibited by sodium azide.1 Publication


  1. KM=35 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=1.529 mM for 2-methoxyphenol1 Publication
  3. KM=1.582 mM for hydroquinone1 Publication
  4. KM=17.6 µM for 2,6-dimethoxyphenol1 Publication
  5. KM=265 µM for pyrogallol1 Publication
  1. Vmax=138.9 µmol/min/mg enzyme toward ABTS1 Publication
  2. Vmax=1.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication
  3. Vmax=9.9 µmol/min/mg enzyme toward hydroquinone1 Publication
  4. Vmax=11.9 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication
  5. Vmax=8.5 µmol/min/mg enzyme toward pyrogallol1 Publication

pH dependencei

Optimum pH is 4.4 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius. Half-life at 70 degrees Celsius is 30 minutes.1 Publication

GO - Molecular functioni

  • hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • lignin catabolic process Source: UniProtKB


Molecular functionOxidoreductase
Biological processLignin degradation
LigandCopper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C1 (EC:
Alternative name(s):
Benzenediol:oxygen oxidoreductase C1By similarity
Diphenol oxidase C1By similarity
Lac C1
Urishiol oxidase C1By similarity
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

  • extracellular region Source: UniProtKB

Keywords - Cellular componenti


PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003817381 – ›63Laccase-C1Add BLAST›63

Post-translational modificationi

Glycosylated; contains 16% carbohydrates.1 Publication



By copper ions.1 Publication


Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the multicopper oxidase family.Sequence analysis


Sequence statusi: Fragments.

P86328-1 [UniParc]FASTAAdd to basket

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Mass (Da):7,148
Last modified:January 11, 2011 - v2

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-adjacent residuesi10 – 11Curated2
Sequence uncertaintyi15L or I1
Sequence uncertaintyi20Q or K1
Sequence uncertaintyi22L or I1
Sequence uncertaintyi23L or I1
Non-adjacent residuesi24 – 25Curated2
Sequence uncertaintyi34L or I1
Sequence uncertaintyi35Q or K1
Sequence uncertaintyi38K or Q1
Non-adjacent residuesi39 – 40Curated2
Sequence uncertaintyi49L or I1
Non-adjacent residuesi50 – 51Curated2
Sequence uncertaintyi51L or I1
Sequence uncertaintyi53L or K1
Sequence uncertaintyi54W or GE1
Sequence uncertaintyi56Q or K1
Sequence uncertaintyi61W or GE1
Sequence uncertaintyi62L or I1
Non-terminal residuei631

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLACC1_CERUI
AccessioniPrimary (citable) accession number: P86328
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: January 11, 2011
Last modified: November 26, 2014
This is version 15 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program



The order of the peptides is unknown.Curated

Keywords - Technical termi

Direct protein sequencing


  1. SIMILARITY comments
    Index of protein domains and families