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Protein

Laccase-C1

Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products (Probable). Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.Curated1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Enzyme regulationi

Inhibited by sodium azide.1 Publication

Kineticsi

  1. KM=35 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=1.529 mM for 2-methoxyphenol1 Publication
  3. KM=1.582 mM for hydroquinone1 Publication
  4. KM=17.6 µM for 2,6-dimethoxyphenol1 Publication
  5. KM=265 µM for pyrogallol1 Publication

Vmax=138.9 µmol/min/mg enzyme toward ABTS1 Publication

Vmax=1.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication

Vmax=9.9 µmol/min/mg enzyme toward hydroquinone1 Publication

Vmax=11.9 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication

Vmax=8.5 µmol/min/mg enzyme toward pyrogallol1 Publication

pH dependencei

Optimum pH is 4.4 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius. Half-life at 70 degrees Celsius is 30 minutes.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C1 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase C1By similarity
Diphenol oxidase C1By similarity
Lac C1
Urishiol oxidase C1By similarity
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

  1. Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›63›63Laccase-C1PRO_0000381738Add
BLAST

Post-translational modificationi

Glycosylated; contains 16% carbohydrates.1 Publication

Expressioni

Inductioni

By copper ions.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the multicopper oxidase family.Sequence Analysis

Sequencei

Sequence statusi: Fragments.

P86328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AIGPVADIDI DVVSLGMVDQ SLLREMHEVS NVALQAMKGS DEYDFNANLR
60
LVLWNQPDFM WLR
Length:63
Mass (Da):7,148
Last modified:January 11, 2011 - v2
Checksum:i2E1D1E2EDDD6131E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi10 – 112Curated
Sequence uncertaintyi15 – 151L or I
Sequence uncertaintyi20 – 201Q or K
Sequence uncertaintyi22 – 221L or I
Sequence uncertaintyi23 – 231L or I
Non-adjacent residuesi24 – 252Curated
Sequence uncertaintyi34 – 341L or I
Sequence uncertaintyi35 – 351Q or K
Sequence uncertaintyi38 – 381K or Q
Non-adjacent residuesi39 – 402Curated
Sequence uncertaintyi49 – 491L or I
Non-adjacent residuesi50 – 512Curated
Sequence uncertaintyi51 – 511L or I
Sequence uncertaintyi53 – 531L or K
Sequence uncertaintyi54 – 541W or GE
Sequence uncertaintyi56 – 561Q or K
Sequence uncertaintyi61 – 611W or GE
Sequence uncertaintyi62 – 621L or I
Non-terminal residuei63 – 631

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
    Lisova Z.A., Lisov A.V., Leontievsky A.A.
    J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
    Strain: VKM F-3196.

Entry informationi

Entry nameiLACC1_CERUI
AccessioniPrimary (citable) accession number: P86328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: January 11, 2011
Last modified: November 26, 2014
This is version 15 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 4.05, its MW is: 75 kDa.

Caution

The order of the peptides is unknown.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.