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P86328

- LACC1_CERUI

UniProt

P86328 - LACC1_CERUI

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Protein

Laccase-C1

Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products (Probable). Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.1 PublicationCurated

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Enzyme regulationi

Inhibited by sodium azide.1 Publication

Kineticsi

  1. KM=35 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=1.529 mM for 2-methoxyphenol1 Publication
  3. KM=1.582 mM for hydroquinone1 Publication
  4. KM=17.6 µM for 2,6-dimethoxyphenol1 Publication
  5. KM=265 µM for pyrogallol1 Publication

Vmax=138.9 µmol/min/mg enzyme toward ABTS1 Publication

Vmax=1.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication

Vmax=9.9 µmol/min/mg enzyme toward hydroquinone1 Publication

Vmax=11.9 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication

Vmax=8.5 µmol/min/mg enzyme toward pyrogallol1 Publication

pH dependencei

Optimum pH is 4.4 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius. Half-life at 70 degrees Celsius is 30 minutes.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C1 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase C1By similarity
Diphenol oxidase C1By similarity
Lac C1
Urishiol oxidase C1By similarity
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›63›63Laccase-C1PRO_0000381738Add
BLAST

Post-translational modificationi

Glycosylated; contains 16% carbohydrates.1 Publication

Expressioni

Inductioni

By copper ions.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the multicopper oxidase family.Sequence Analysis

Sequencei

Sequence statusi: Fragments.

P86328-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AIGPVADIDI DVVSLGMVDQ SLLREMHEVS NVALQAMKGS DEYDFNANLR
60
LVLWNQPDFM WLR
Length:63
Mass (Da):7,148
Last modified:January 11, 2011 - v2
Checksum:i2E1D1E2EDDD6131E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi10 – 112Curated
Sequence uncertaintyi15 – 151L or I
Sequence uncertaintyi20 – 201Q or K
Sequence uncertaintyi22 – 221L or I
Sequence uncertaintyi23 – 231L or I
Non-adjacent residuesi24 – 252Curated
Sequence uncertaintyi34 – 341L or I
Sequence uncertaintyi35 – 351Q or K
Sequence uncertaintyi38 – 381K or Q
Non-adjacent residuesi39 – 402Curated
Sequence uncertaintyi49 – 491L or I
Non-adjacent residuesi50 – 512Curated
Sequence uncertaintyi51 – 511L or I
Sequence uncertaintyi53 – 531L or K
Sequence uncertaintyi54 – 541W or GE
Sequence uncertaintyi56 – 561Q or K
Sequence uncertaintyi61 – 611W or GE
Sequence uncertaintyi62 – 621L or I
Non-terminal residuei63 – 631

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
    Lisova Z.A., Lisov A.V., Leontievsky A.A.
    J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
    Strain: VKM F-3196.

Entry informationi

Entry nameiLACC1_CERUI
AccessioniPrimary (citable) accession number: P86328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: January 11, 2011
Last modified: November 26, 2014
This is version 15 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 4.05, its MW is: 75 kDa.

Caution

The order of the peptides is unknown.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3