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P86328

- LACC1_CERUI

UniProt

P86328 - LACC1_CERUI

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Protein
Laccase-C1
Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products Inferred. Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.2 Publications

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.2 Publications

Cofactori

Binds 4 copper ions per monomer By similarity.By similarity

Enzyme regulationi

Inhibited by sodium azide.2 Publications

Kineticsi

  1. KM=35 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=1.529 mM for 2-methoxyphenol1 Publication
  3. KM=1.582 mM for hydroquinone1 Publication
  4. KM=17.6 µM for 2,6-dimethoxyphenol1 Publication
  5. KM=265 µM for pyrogallol

Vmax=138.9 µmol/min/mg enzyme toward ABTS

Vmax=1.4 µmol/min/mg enzyme toward 2-methoxyphenol

Vmax=9.9 µmol/min/mg enzyme toward hydroquinone

Vmax=11.9 µmol/min/mg enzyme toward 2,6-dimethoxyphenol

Vmax=8.5 µmol/min/mg enzyme toward pyrogallol

pH dependencei

Optimum pH is 4.4 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius. Half-life at 70 degrees Celsius is 30 minutes.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C1 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase C1
Diphenol oxidase C1
Lac C1
Urishiol oxidase C1
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›63›63Laccase-C1
PRO_0000381738Add
BLAST

Post-translational modificationi

Glycosylated; contains 16% carbohydrates.1 Publication

Expressioni

Inductioni

By copper ions.2 Publications

Interactioni

Subunit structurei

Monomer.2 Publications

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragments.

P86328-1 [UniParc]FASTAAdd to Basket

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AIGPVADIDI DVVSLGMVDQ SLLREMHEVS NVALQAMKGS DEYDFNANLR   50
LVLWNQPDFM WLR 63
Length:63
Mass (Da):7,148
Last modified:January 11, 2011 - v2
Checksum:i2E1D1E2EDDD6131E
GO

Sequence uncertainty

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence uncertaintyi15 – 151L or I
Sequence uncertaintyi20 – 201Q or K
Sequence uncertaintyi22 – 221L or I
Sequence uncertaintyi23 – 231L or I
Sequence uncertaintyi34 – 341L or I1 Publication
Sequence uncertaintyi35 – 351Q or K1 Publication
Sequence uncertaintyi38 – 381K or Q1 Publication
Sequence uncertaintyi49 – 491L or I1 Publication
Sequence uncertaintyi51 – 511L or I1 Publication
Sequence uncertaintyi53 – 531L or K1 Publication
Sequence uncertaintyi54 – 541W or GE1 Publication
Sequence uncertaintyi56 – 561Q or K1 Publication
Sequence uncertaintyi61 – 611W or GE1 Publication
Sequence uncertaintyi62 – 621L or I1 Publication

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi10 – 112
Non-adjacent residuesi24 – 252
Non-adjacent residuesi39 – 402
Non-adjacent residuesi50 – 512

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei63 – 631

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
    Lisova Z.A., Lisov A.V., Leontievsky A.A.
    J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
    Strain: VKM F-3196.

Entry informationi

Entry nameiLACC1_CERUI
AccessioniPrimary (citable) accession number: P86328
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: January 11, 2011
Last modified: February 19, 2014
This is version 13 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 4.05, its MW is: 75 kDa.

Caution

The order of the peptides is unknown.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3