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P86328

- LACC1_CERUI

UniProt

P86328 - LACC1_CERUI

Protein

Laccase-C1

Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Lignin degradation and detoxification of lignin-derived products Probable. Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.1 PublicationCurated

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Enzyme regulationi

    Inhibited by sodium azide.1 Publication

    Kineticsi

    1. KM=35 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
    2. KM=1.529 mM for 2-methoxyphenol1 Publication
    3. KM=1.582 mM for hydroquinone1 Publication
    4. KM=17.6 µM for 2,6-dimethoxyphenol1 Publication
    5. KM=265 µM for pyrogallol1 Publication

    Vmax=138.9 µmol/min/mg enzyme toward ABTS1 Publication

    Vmax=1.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication

    Vmax=9.9 µmol/min/mg enzyme toward hydroquinone1 Publication

    Vmax=11.9 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication

    Vmax=8.5 µmol/min/mg enzyme toward pyrogallol1 Publication

    pH dependencei

    Optimum pH is 4.4 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius. Half-life at 70 degrees Celsius is 30 minutes.1 Publication

    GO - Molecular functioni

    1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-C1 (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase C1By similarity
    Diphenol oxidase C1By similarity
    Lac C1
    Urishiol oxidase C1By similarity
    OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
    Taxonomic identifieri90312 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›63›63Laccase-C1PRO_0000381738Add
    BLAST

    Post-translational modificationi

    Glycosylated; contains 16% carbohydrates.1 Publication

    Expressioni

    Inductioni

    By copper ions.1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Sequence Analysis

    Sequencei

    Sequence statusi: Fragments.

    P86328-1 [UniParc]FASTAAdd to Basket

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    AIGPVADIDI DVVSLGMVDQ SLLREMHEVS NVALQAMKGS DEYDFNANLR   50
    LVLWNQPDFM WLR 63
    Length:63
    Mass (Da):7,148
    Last modified:January 11, 2011 - v2
    Checksum:i2E1D1E2EDDD6131E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi10 – 112Curated
    Sequence uncertaintyi15 – 151L or I
    Sequence uncertaintyi20 – 201Q or K
    Sequence uncertaintyi22 – 221L or I
    Sequence uncertaintyi23 – 231L or I
    Non-adjacent residuesi24 – 252Curated
    Sequence uncertaintyi34 – 341L or I
    Sequence uncertaintyi35 – 351Q or K
    Sequence uncertaintyi38 – 381K or Q
    Non-adjacent residuesi39 – 402Curated
    Sequence uncertaintyi49 – 491L or I
    Non-adjacent residuesi50 – 512Curated
    Sequence uncertaintyi51 – 511L or I
    Sequence uncertaintyi53 – 531L or K
    Sequence uncertaintyi54 – 541W or GE
    Sequence uncertaintyi56 – 561Q or K
    Sequence uncertaintyi61 – 611W or GE
    Sequence uncertaintyi62 – 621L or I
    Non-terminal residuei63 – 631

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
      Lisova Z.A., Lisov A.V., Leontievsky A.A.
      J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
      Strain: VKM F-3196.

    Entry informationi

    Entry nameiLACC1_CERUI
    AccessioniPrimary (citable) accession number: P86328
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: January 11, 2011
    Last modified: October 1, 2014
    This is version 14 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    On the 2D-gel the determined pI of this protein is: 4.05, its MW is: 75 kDa.

    Caution

    The order of the peptides is unknown.Curated

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3