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P86327

- LACC2_CERUI

UniProt

P86327 - LACC2_CERUI

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Protein
Laccase-C2
Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products Inferred. Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.2 Publications

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.2 Publications

Cofactori

Binds 4 copper ions per monomer By similarity.By similarity

Enzyme regulationi

Inhibited by sodium azide.2 Publications

Kineticsi

  1. KM=10 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=127 µM for 2-methoxyphenol
  3. KM=518 µM for hydroquinone
  4. KM=4.9 µM for 2,6-dimethoxyphenol
  5. KM=140 µM for pyrogallol

Vmax=92.6 µmol/min/mg enzyme toward 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)

Vmax=2.4 µmol/min/mg enzyme toward 2-methoxyphenol

Vmax=16.4 µmol/min/mg enzyme toward hydroquinone

Vmax=0.1 µmol/min/mg enzyme toward 2,6-dimethoxyphenol

Vmax=8.3 µmol/min/mg enzyme toward pyrogallol

pH dependencei

Optimum pH is 3.8 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius. Half-life at 60 degrees Celsius is 20 minutes. Half-life at 70 degrees Celsius is 3 minutes.

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C2 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase C2
Diphenol oxidase C2
Lac C2
Urishiol oxidase C2
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›62›62Laccase-C2
PRO_0000381739Add
BLAST

Post-translational modificationi

Glycosylated; contains 6% carbohydrates.1 Publication

Expressioni

Inductioni

By copper ions.2 Publications

Interactioni

Subunit structurei

Monomer.2 Publications

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragments.

P86327-1 [UniParc]FASTAAdd to Basket

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AIGPVADLDI VNDQLTDASM IKWDCLPYDV RSPNPLGTTG FAGGNYRSVP   50
ADLHLTTLAD PR 62
Length:62
Mass (Da):6,626
Last modified:September 1, 2009 - v1
Checksum:i9A35C2CCBFB7F179
GO

Sequence uncertainty

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence uncertaintyi14 – 141Q or K1 Publication
Sequence uncertaintyi15 – 151L or I1 Publication
Sequence uncertaintyi22 – 221K or Q1 Publication
Sequence uncertaintyi23 – 231W or GE1 Publication
Sequence uncertaintyi26 – 261L or I1 Publication
Sequence uncertaintyi36 – 361L or I1 Publication
Sequence uncertaintyi53 – 531L or I1 Publication
Sequence uncertaintyi55 – 551L or I1 Publication
Sequence uncertaintyi58 – 581L or I1 Publication

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi10 – 112
Non-adjacent residuesi22 – 232
Non-adjacent residuesi31 – 322
Non-adjacent residuesi47 – 482

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei62 – 621

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
    Lisova Z.A., Lisov A.V., Leontievsky A.A.
    J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
    Strain: VKM F-3196.

Entry informationi

Entry nameiLACC2_CERUI
AccessioniPrimary (citable) accession number: P86327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: February 19, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 3.85, its MW is: 67 kDa.1 Publication

Caution

The order of the peptides is unknown.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3