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P86327

- LACC2_CERUI

UniProt

P86327 - LACC2_CERUI

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Protein

Laccase-C2

Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products (Probable). Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.1 PublicationCurated

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Enzyme regulationi

Inhibited by sodium azide.1 Publication

Kineticsi

  1. KM=10 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=127 µM for 2-methoxyphenol1 Publication
  3. KM=518 µM for hydroquinone1 Publication
  4. KM=4.9 µM for 2,6-dimethoxyphenol1 Publication
  5. KM=140 µM for pyrogallol1 Publication

Vmax=92.6 µmol/min/mg enzyme toward 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication

Vmax=2.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication

Vmax=16.4 µmol/min/mg enzyme toward hydroquinone1 Publication

Vmax=0.1 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication

Vmax=8.3 µmol/min/mg enzyme toward pyrogallol1 Publication

pH dependencei

Optimum pH is 3.8 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius. Half-life at 60 degrees Celsius is 20 minutes. Half-life at 70 degrees Celsius is 3 minutes.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C2 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase C2By similarity
Diphenol oxidase C2By similarity
Lac C2
Urishiol oxidase C2By similarity
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›62›62Laccase-C2PRO_0000381739Add
BLAST

Post-translational modificationi

Glycosylated; contains 6% carbohydrates.1 Publication

Expressioni

Inductioni

By copper ions.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the multicopper oxidase family.Sequence Analysis

Sequencei

Sequence statusi: Fragments.

P86327-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AIGPVADLDI VNDQLTDASM IKWDCLPYDV RSPNPLGTTG FAGGNYRSVP
60
ADLHLTTLAD PR
Length:62
Mass (Da):6,626
Last modified:September 1, 2009 - v1
Checksum:i9A35C2CCBFB7F179
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi10 – 112Curated
Sequence uncertaintyi14 – 141Q or K
Sequence uncertaintyi15 – 151L or I
Non-adjacent residuesi22 – 232Curated
Sequence uncertaintyi22 – 221K or Q
Sequence uncertaintyi23 – 231W or GE
Sequence uncertaintyi26 – 261L or I
Non-adjacent residuesi31 – 322Curated
Sequence uncertaintyi36 – 361L or I
Non-adjacent residuesi47 – 482Curated
Sequence uncertaintyi53 – 531L or I
Sequence uncertaintyi55 – 551L or I
Sequence uncertaintyi58 – 581L or I
Non-terminal residuei62 – 621

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
    Lisova Z.A., Lisov A.V., Leontievsky A.A.
    J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
    Strain: VKM F-3196.

Entry informationi

Entry nameiLACC2_CERUI
AccessioniPrimary (citable) accession number: P86327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 26, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 3.85, its MW is: 67 kDa.

Caution

The order of the peptides is unknown.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3