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P86327

- LACC2_CERUI

UniProt

P86327 - LACC2_CERUI

Protein

Laccase-C2

Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Lignin degradation and detoxification of lignin-derived products Probable. Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.1 PublicationCurated

    Catalytic activityi

    4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

    Cofactori

    Binds 4 copper ions per monomer.By similarity

    Enzyme regulationi

    Inhibited by sodium azide.1 Publication

    Kineticsi

    1. KM=10 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
    2. KM=127 µM for 2-methoxyphenol1 Publication
    3. KM=518 µM for hydroquinone1 Publication
    4. KM=4.9 µM for 2,6-dimethoxyphenol1 Publication
    5. KM=140 µM for pyrogallol1 Publication

    Vmax=92.6 µmol/min/mg enzyme toward 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication

    Vmax=2.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication

    Vmax=16.4 µmol/min/mg enzyme toward hydroquinone1 Publication

    Vmax=0.1 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication

    Vmax=8.3 µmol/min/mg enzyme toward pyrogallol1 Publication

    pH dependencei

    Optimum pH is 3.8 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius. Half-life at 60 degrees Celsius is 20 minutes. Half-life at 70 degrees Celsius is 3 minutes.1 Publication

    GO - Molecular functioni

    1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. lignin catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Keywords - Ligandi

    Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase-C2 (EC:1.10.3.2)
    Alternative name(s):
    Benzenediol:oxygen oxidoreductase C2By similarity
    Diphenol oxidase C2By similarity
    Lac C2
    Urishiol oxidase C2By similarity
    OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
    Taxonomic identifieri90312 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›62›62Laccase-C2PRO_0000381739Add
    BLAST

    Post-translational modificationi

    Glycosylated; contains 6% carbohydrates.1 Publication

    Expressioni

    Inductioni

    By copper ions.1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the multicopper oxidase family.Sequence Analysis

    Sequencei

    Sequence statusi: Fragments.

    P86327-1 [UniParc]FASTAAdd to Basket

    « Hide

    AIGPVADLDI VNDQLTDASM IKWDCLPYDV RSPNPLGTTG FAGGNYRSVP   50
    ADLHLTTLAD PR 62
    Length:62
    Mass (Da):6,626
    Last modified:September 1, 2009 - v1
    Checksum:i9A35C2CCBFB7F179
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi10 – 112Curated
    Sequence uncertaintyi14 – 141Q or K
    Sequence uncertaintyi15 – 151L or I
    Non-adjacent residuesi22 – 232Curated
    Sequence uncertaintyi22 – 221K or Q
    Sequence uncertaintyi23 – 231W or GE
    Sequence uncertaintyi26 – 261L or I
    Non-adjacent residuesi31 – 322Curated
    Sequence uncertaintyi36 – 361L or I
    Non-adjacent residuesi47 – 482Curated
    Sequence uncertaintyi53 – 531L or I
    Sequence uncertaintyi55 – 551L or I
    Sequence uncertaintyi58 – 581L or I
    Non-terminal residuei62 – 621

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
      Lisova Z.A., Lisov A.V., Leontievsky A.A.
      J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
      Strain: VKM F-3196.

    Entry informationi

    Entry nameiLACC2_CERUI
    AccessioniPrimary (citable) accession number: P86327
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 1, 2009
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 14 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    On the 2D-gel the determined pI of this protein is: 3.85, its MW is: 67 kDa.

    Caution

    The order of the peptides is unknown.Curated

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3