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Protein

Laccase-C2

Gene
N/A
Organism
Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products (Probable). Oxidation of a broad range of substrates including mono-, di- and polyphenols, aromatic amines and methoxy-substituted phenols accompanied by reduction of oxygen to water.1 PublicationCurated

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Cofactori

Cu cationBy similarityNote: Binds 4 Cu cations per monomer.By similarity

Enzyme regulationi

Inhibited by sodium azide.1 Publication

Kineticsi

  1. KM=10 µM for 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication
  2. KM=127 µM for 2-methoxyphenol1 Publication
  3. KM=518 µM for hydroquinone1 Publication
  4. KM=4.9 µM for 2,6-dimethoxyphenol1 Publication
  5. KM=140 µM for pyrogallol1 Publication

Vmax=92.6 µmol/min/mg enzyme toward 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)1 Publication

Vmax=2.4 µmol/min/mg enzyme toward 2-methoxyphenol1 Publication

Vmax=16.4 µmol/min/mg enzyme toward hydroquinone1 Publication

Vmax=0.1 µmol/min/mg enzyme toward 2,6-dimethoxyphenol1 Publication

Vmax=8.3 µmol/min/mg enzyme toward pyrogallol1 Publication

pH dependencei

Optimum pH is 3.8 with 2,6-dimethoxyphenol as substrate, and less than 2.7 with ABTS as substrate.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius. Half-life at 60 degrees Celsius is 20 minutes. Half-life at 70 degrees Celsius is 3 minutes.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Keywords - Ligandi

Copper, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase-C2 (EC:1.10.3.2)
Alternative name(s):
Benzenediol:oxygen oxidoreductase C2By similarity
Diphenol oxidase C2By similarity
Lac C2
Urishiol oxidase C2By similarity
OrganismiCerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifieri90312 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPolyporaceaeCerrena

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›62›62Laccase-C2PRO_0000381739Add
BLAST

Post-translational modificationi

Glycosylated; contains 6% carbohydrates.1 Publication

Expressioni

Inductioni

By copper ions.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the multicopper oxidase family.Sequence Analysis

Sequencei

Sequence statusi: Fragments.

P86327-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AIGPVADLDI VNDQLTDASM IKWDCLPYDV RSPNPLGTTG FAGGNYRSVP
60
ADLHLTTLAD PR
Length:62
Mass (Da):6,626
Last modified:September 1, 2009 - v1
Checksum:i9A35C2CCBFB7F179
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi10 – 112Curated
Sequence uncertaintyi14 – 141Q or K
Sequence uncertaintyi15 – 151L or I
Non-adjacent residuesi22 – 232Curated
Sequence uncertaintyi22 – 221K or Q
Sequence uncertaintyi23 – 231W or GE
Sequence uncertaintyi26 – 261L or I
Non-adjacent residuesi31 – 322Curated
Sequence uncertaintyi36 – 361L or I
Non-adjacent residuesi47 – 482Curated
Sequence uncertaintyi53 – 531L or I
Sequence uncertaintyi55 – 551L or I
Sequence uncertaintyi58 – 581L or I
Non-terminal residuei62 – 621

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties."
    Lisova Z.A., Lisov A.V., Leontievsky A.A.
    J. Basic Microbiol. 50:72-82(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, GLYCOSYLATION.
    Strain: VKM F-3196.

Entry informationi

Entry nameiLACC2_CERUI
AccessioniPrimary (citable) accession number: P86327
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 26, 2014
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 3.85, its MW is: 67 kDa.

Caution

The order of the peptides is unknown.Curated

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.