ID   HYAL_PHOKE              Reviewed;          32 AA.
AC   P86274;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   07-OCT-2020, entry version 20.
DE   RecName: Full=Hyaluronidase-Pk1a {ECO:0000250|UniProtKB:Q08169};
DE            Short=Hya {ECO:0000303|Ref.1};
DE            EC=3.2.1.35;
DE   AltName: Full=Hyaluronoglucosaminidase {ECO:0000250|UniProtKB:Q08169};
DE   Flags: Fragment;
OS   Phoneutria keyserlingi (Brazilian wandering spider) (Ctenus keyserlingii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX   NCBI_TaxID=272754;
RN   [1]
RP   PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Venom {ECO:0000269|Ref.1};
RA   Richardson M., Borges M.H., Souza I.A., Cordeiro M.N.;
RT   "Hyaluronidase enzyme in venom of spider P.keyserlinigi (wandering).";
RL   Submitted (APR-2009) to UniProtKB.
CC   -!- FUNCTION: Hydrolyzes high molecular weight hyaluronic acid to produce
CC       small oligosaccharides. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC         glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC         Evidence={ECO:0000269|Ref.1};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.1}.
CC   -!- MISCELLANEOUS: On the 2D-gel the MW of this protein is: 37 kDa.
CC       {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family. {ECO:0000255}.
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DR   ArachnoServer; AS001206; Hyaluronidase-Pk1a (fragment).
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; Glycosidase;
KW   Hydrolase; Secreted.
FT   CHAIN           1..>32
FT                   /note="Hyaluronidase-Pk1a"
FT                   /id="PRO_0000376024"
FT   CARBOHYD        23
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        12..?
FT                   /evidence="ECO:0000250|UniProtKB:Q08169"
FT   NON_TER         32
FT                   /evidence="ECO:0000303|Ref.1"
SQ   SEQUENCE   32 AA;  3879 MW;  6CC71A1D7F06AB76 CRC64;
     FEVYWNVPTL QCRAVYKMIF KLNRTYGIQX NA
//