Reviewed, UniProtKB/Swiss-Prot P86274 (HUGA_PHOKE)

Names and origin

Protein names	Recommended name:     Hyaluronidase       Short name=Hya     EC=3.2.1.35 Alternative name(s):     Hyaluronoglucosaminidase
Organism	Phoneutria keyserlingi (Brazilian wandering spider) (Ctenus keyserlingii)
Taxonomic identifier	272754 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Araneae › Araneomorphae › Entelegynae › Lycosoidea › Ctenidae › Phoneutria

Protein attributes

Sequence length	32 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.1
Subcellular location	Secreted. Ref.1
Tissue specificity	Expressed by the venom gland. Ref.1
Miscellaneous	On the 2D-gel the MW of this protein is: 37 kDa. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 56 family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›32	›32	Hyaluronidase		PRO_0000376024
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Disulfide bond	12 ↔ ?		By similarity UniProtKB Q08169
Experimental info
	Non-terminal residue	32	1

Sequences

	Sequence		Length	Mass (Da)
	P86274-1 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 6CC71A1D7F06AB76	FASTA	32	3,879
	10 20 30 FEVYWNVPTL QCRAVYKMIF KLNRTYGIQX NA

References

[1]	"Hyaluronidase enzyme in venom of spider P.keyserlinigi (wandering)." Richardson M., Borges M.H., Souza I.A., Cordeiro M.N. Submitted (APR-2009) to UniProtKB Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

HUGA_PHOKE

Accession

Primary (citable) accession number: P86274

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	September 22, 2009
This is version 3 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)