Potassium channel toxin alpha-KTx 21.1

Functionⁱ

Reversibly and voltage-independently blocks voltage-gated potassium channels rKv1.2/KCNA2 (73%) (IC₅₀=196 nM), hKv1.3/KCNA3 (50%) (IC₅₀=508 nM), Shaker IR (30%), rKv1.6/KCNA6 (22%) (at 0.5 µM). Interaction of Ts15 with Kv1.3/KCNA3 is stronger than its interaction with Kv1.2/KCNA2.1 Publication

Miscellaneous

Does not block voltage-gated sodium channels rNav1.4/SCN4A, hNav1.5/SCN5A, mNav1.6/SCN8A and rNav1.8/SCN10A, and Kv1.1/KCNA1, rKv1.4/KCNA4, rKv1.5/KCNA5, rKv2.1/KCNB1, hKv3.1/KCNC1, rKv4.2/KCND2, rKv4.3/KCND3 and hERG potassium channels, when tested at a concentration of 0.5 µM.1 Publication

GO - Biological processⁱ

defense response Source: InterPro

Complete GO annotation...

Keywordsⁱ

Molecular function	Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Molecular function

Ion channel impairing toxin, Neurotoxin, Potassium channel impairing toxin, Toxin, Voltage-gated potassium channel impairing toxin

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Potassium channel toxin alpha-KTx 21.1 Alternative name(s): Tityustoxin-15 Short name: Ts15
Organismⁱ	Tityus serrulatus (Brazilian scorpion)
Taxonomic identifierⁱ	6887 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Tityus

Subcellular locationⁱ

Secreted
3 Publications
Manual assertion based on experiment inⁱ
- Ref.1
  "Purification and characterization of Ts15, the first member of a new alpha-KTX subfamily from the venom of the Brazilian scorpion Tityus serrulatus."
  Cologna C.T., Peigneur S., Rosa J.C., Selistre-de-Araujo H.S., Varanda W.A., Tytgat J., Arantes E.C.
  Toxicon 58:54-61(2011) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
- Ref.2
  "Moving pieces in a venomic puzzle: unveiling post-translationally modified toxins from Tityus serrulatus."
  Verano-Braga T., Dutra A.A., Leon I.R., Melo-Braga M.N., Roepstorff P., Pimenta A.M., Kjeldsen F.
  J. Proteome Res. 12:3460-3470(2013) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 22-36, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
- Ref.3
  "Influence of post-starvation extraction time and prey-specific diet in Tityus serrulatus scorpion venom composition and hyaluronidase activity."
  Pucca M.B., Amorim F.G., Cerni F.A., Bordon K.C.F., Cardoso I.A., Anjolette F.A., Arantes E.C.
  Toxicon 90:326-336(2014) [PubMed] [Europe PMC] [Abstract]
  Cited for: PROTEIN SEQUENCE OF 7-12, SUBCELLULAR LOCATION.

GO - Cellular componentⁱ

extracellular region Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Secreted

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
PeptideⁱPRO_0000376048	1 – 36	Potassium channel toxin alpha-KTx 21.1Add BLAST		36

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Disulfide bondⁱ	6 ↔ 26	By similarity
Disulfide bondⁱ	11 ↔ 31	By similarity
Disulfide bondⁱ	15 ↔ 33	By similarity
Glycosylationⁱ	27	N-linked (GlcNAc...) asparagine	1

Keywords - PTMⁱ

Disulfide bond, Glycoprotein

Expressionⁱ

Tissue specificityⁱ

Expressed by the venom gland.1 Publication

Structureⁱ

3D structure databases

SMRⁱ	P86270.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 21 subfamily.Curated

Family and domain databases

Gene3Dⁱ	3.30.30.10. 1 hit.
InterProⁱ	View protein in InterPro IPR003614. Scorpion_toxin-like.

Sequenceⁱ

Sequence statusⁱ: Complete.

P86270-1 [UniParc]FASTA Add to basket

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        10         20         30 
GKFGKCKPNI CAKTCQTEKG KGMGYCNKTE CVCSEW

Length:36

Mass (Da):3,962

Last modified:May 26, 2009 - v1

Checksum:ⁱ7DE0232A5D53CBAE

GO

Mass spectrometryⁱ

Molecular mass is 3956 Da from positions 1 - 36. Determined by ESI. 1 Publication

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

3D structure databases

SMRⁱ	P86270.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

Gene3Dⁱ	3.30.30.10. 1 hit.
InterProⁱ	View protein in InterPro IPR003614. Scorpion_toxin-like.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KA211_TITSE
Accessionⁱ	P86270Primary (citable) accession number: P86270
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
	Last sequence update:	May 26, 2009
	Last modified:	May 10, 2017
	This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Animal Toxin Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Direct protein sequencing

Documents

Scorpion potassium channel toxins
Nomenclature of scorpion potassium channel toxins and list of entries
SIMILARITY comments
Index of protein domains and families

UniProtKB - P86270 (KA211_TITSE)

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Functionⁱ

Miscellaneous

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Amino acid modifications

Keywords - PTMⁱ

Expressionⁱ

Tissue specificityⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Mass spectrometryⁱ

Similar proteinsⁱ

Cross-referencesⁱ

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P86270 (KA211_TITSE)

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Potassium channel toxin alpha-KTx 21.1

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports information derived from mass spectrometry experiments done on the entire protein or on biologically active derived peptide(s).<p><a href='/help/mass_spectrometry' target='_top'>More...</a></p>Mass spectrometryi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Mass spectrometryⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ