P86270 (KA211_TITSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Potassium channel toxin alpha-KTx 21.1 Alternative name(s): Tityustoxin-15 Short name=Ts15
Organism	Tityus serrulatus (Brazilian scorpion)
Taxonomic identifier	6887 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Tityus

Protein attributes

Sequence length	36 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reversibly and voltage-independently blocks voltage-gated potassium channels rKv1.2/KCNA2 (73%) (IC50=196 nM), hKv1.3/KCNA3 (50%) (IC50=508 nM), Shaker IR (30%), rKv1.6/KCNA6 (22%) (at 0.5 µM). Interaction of Ts15 with Kv1.3/KCNA3 is stronger than its interaction with Kv1.2/KCNA2. Ref.1
Subcellular location	Secreted Ref.1 Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1 Ref.1
Miscellaneous	Does not block voltage-gated sodium channels rNav1.4/SCN4A, hNav1.5/SCN5A, mNav1.6/SCN8A and rNav1.8/SCN10A, and Kv1.1/KCNA1, rKv1.4/KCNA4, rKv1.5/KCNA5, rKv2.1/KCNB1, hKv3.1/KCNC1, rKv4.2/KCND2, rKv4.3/KCND3 and hERG potassium channels, when tested at a concentration of 0.5 µM (Ref.1).
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 21 subfamily.
Mass spectrometry	Molecular mass is 3956 Da from positions 1 - 36. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	potassium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 36	36	Potassium channel toxin alpha-KTx 21.1 Ref.1		PRO_0000376048
Amino acid modifications
	Disulfide bond	6 ↔ 26		By similarity UniProtKB Q8I0L5
	Disulfide bond	11 ↔ 31		By similarity UniProtKB Q8I0L5
	Disulfide bond	15 ↔ 33		By similarity UniProtKB Q8I0L5

Sequences

	Sequence		Length	Mass (Da)
	P86270 [UniParc]. Last modified May 26, 2009. Version 1. Checksum: 7DE0232A5D53CBAE	FASTA	36	3,962
	10 20 30 GKFGKCKPNI CAKTCQTEKG KGMGYCNKTE CVCSEW

References

[1]

"Purification and characterization of Ts15, the first member of a new alpha-KTX subfamily from the venom of the Brazilian scorpion Tityus serrulatus." Cologna C.T., Peigneur S., Rosa J.C., Selistre-de-Araujo H.S., Varanda W.A., Tytgat J., Arantes E.C. Toxicon 58:54-61(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS01138. SCORP_SHORT_TOXIN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KA211_TITSE

Accession

Primary (citable) accession number: P86270

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	May 26, 2009
Last modified:	March 6, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program