P86250 (NDUS2_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-49kD
Short name=CI-49kD
NADH-ubiquinone oxidoreductase 49 kDa subunit

Gene names

Name:

NDUFS2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	138 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. UniProtKB P17694
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. UniProtKB P17694 NADH + acceptor = NAD+ + reduced acceptor. UniProtKB P17694
Cofactor	Binds 1 4Fe-4S cluster By similarity. UniProtKB P17694
Subunit structure	Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme. Interacts with NDUFAF3 By similarity. UniProtKB O75306
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity UniProtKB P17694.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 138	›138	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial		PRO_0000394421
Experimental info
	Non-adjacent residues	11 – 12	2
	Non-adjacent residues	20 – 21	2
	Non-adjacent residues	29 – 30	2
	Non-adjacent residues	74 – 75	2
	Non-adjacent residues	94 – 95	2
	Non-adjacent residues	115 – 116	2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P86250 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: 19CF54EF543A6495	FASTA	138	15,937
	10 20 30 40 50 60 TYLQALPYFD RLLNIQPPPR EKMFEFYERM HAAYIRPGGV HQDLPLGLMD DIYEFSKNFS 70 80 90 100 110 120 LRIDEVEEML TNNRKTQPYD VYDQVEFDVP IGSRLYTEGY QVPPGATYTA IEAPKGHMLA 130 DVVAIIGTQD IVFGEIDR

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P86250.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 2 hits. [Graphical view]
PROSITE	PS00535. COMPLEX1_49K. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_MESAU

Accession

Primary (citable) accession number: P86250

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	October 3, 2012
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program