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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

NDUFS2

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NAD binding Source: InterPro
  4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  5. quinone binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialBy similarity (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kDBy similarity
Short name:
CI-49kDBy similarity
NADH-ubiquinone oxidoreductase 49 kDa subunitBy similarity
Gene namesi
Name:NDUFS2By similarity
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 138›138NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialPRO_0000394421Add
BLAST

Proteomic databases

PRIDEiP86250.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme. Interacts with NDUFAF3 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Sequence Analysis

Family and domain databases

InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 2 hits.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.

Sequencei

Sequence statusi: Fragments.

P86250-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
TYLQALPYFD RLLNIQPPPR EKMFEFYERM HAAYIRPGGV HQDLPLGLMD
60 70 80 90 100
DIYEFSKNFS LRIDEVEEML TNNRKTQPYD VYDQVEFDVP IGSRLYTEGY
110 120 130
QVPPGATYTA IEAPKGHMLA DVVAIIGTQD IVFGEIDR
Length:138
Mass (Da):15,937
Last modified:June 15, 2010 - v1
Checksum:i19CF54EF543A6495
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-adjacent residuesi11 – 122Curated
Non-adjacent residuesi20 – 212Curated
Non-adjacent residuesi29 – 302Curated
Non-adjacent residuesi74 – 752Curated
Non-adjacent residuesi94 – 952Curated
Non-adjacent residuesi115 – 1162Curated

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP86250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 2 hits.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa."
    Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.
    Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNDUS2_MESAU
AccessioniPrimary (citable) accession number: P86250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: January 7, 2015
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.