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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial

Gene

NDUFS2

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialBy similarity (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-49kDBy similarity
Short name:
CI-49kDBy similarity
NADH-ubiquinone oxidoreductase 49 kDa subunitBy similarity
Gene namesi
Name:NDUFS2By similarity
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000394421‹1 – 138NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrialAdd BLAST›138

Post-translational modificationi

Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex.By similarity

Proteomic databases

PRIDEiP86250.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. Component of the iron-sulfur (IP) fragment of the enzyme. Interacts with NDUFAF3. Interacts with NDUFAF7.By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Sequence analysis

Family and domain databases

InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 2 hits.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.

Sequencei

Sequence statusi: Fragments.

P86250-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
TYLQALPYFD RLLNIQPPPR EKMFEFYERM HAAYIRPGGV HQDLPLGLMD
60 70 80 90 100
DIYEFSKNFS LRIDEVEEML TNNRKTQPYD VYDQVEFDVP IGSRLYTEGY
110 120 130
QVPPGATYTA IEAPKGHMLA DVVAIIGTQD IVFGEIDR
Length:138
Mass (Da):15,937
Last modified:June 15, 2010 - v1
Checksum:i19CF54EF543A6495
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-adjacent residuesi11 – 12Curated2
Non-adjacent residuesi20 – 21Curated2
Non-adjacent residuesi29 – 30Curated2
Non-adjacent residuesi74 – 75Curated2
Non-adjacent residuesi94 – 95Curated2
Non-adjacent residuesi115 – 116Curated2

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP86250.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 2 hits.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS2_MESAU
AccessioniPrimary (citable) accession number: P86250
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: November 2, 2016
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.