P86243 (TOMT_PANTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transmembrane O-methyltransferase
EC=2.1.1.6

Alternative name(s):
Catechol O-methyltransferase 2
Protein LRTOMT2

Gene names

Name:	LRTOMT
Synonyms:	COMT2, TOMT

Organism

Pan troglodytes (Chimpanzee) [Reference proteome]

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Pan

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function By similarity. UniProtKB P21964 UniProtKB Q8WZ04
Catalytic activity	S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol. UniProtKB P21964
Subcellular location	Isoform 1: Membrane; Single-pass membrane protein Potential. Isoform 2: Cytoplasm By similarity UniProtKB A1Y9I9.
Miscellaneous	In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene. Ref.1
Sequence similarities	Belongs to the methyltransferase superfamily. Catechol-O-methyltransferase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism Hearing Neurotransmitter degradation
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW sensory perception of sound Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	catechol O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: P86243-1)

Also known as: D';

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: P86243-2)

Also known as: E';

The sequence of this isoform differs from the canonical sequence as follows:
     28-67: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Transmembrane O-methyltransferase		PRO_0000372487
Regions
	Transmembrane	31 – 51	21	Helical; Potential
Natural variations
	Alternative sequence	28 – 67	40	Missing in isoform 2. Ref.1		VSP_037158

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (D') [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 171964FA01FA2E67	FASTA	291	32,124
	10 20 30 40 50 60 MGTPWRKRKG IAGPGLPHLS CALVLQPRAQ VGTMSPAIAL AFLPLVVTLL VRYRHYFRLL 70 80 90 100 110 120 VRTVLLRSLR DCLSGLRIEE RAFSYVLTHA LPGDPGHILT TLDHWSSCCE YLSHMGPVKG 130 140 150 160 170 180 QILMRLVEEK APACVLELGT YCGYSTLLIA RALPPGGRLL TVERDPRTAA VAEKLIRLAG 190 200 210 220 230 240 FDEHMVELIV GSSEDVIPCL RTQYQLSRAD LVLLAHRPRC YLRDLQLLEA HALLPAGATV 250 260 270 280 290 LADHVLFPGA PRFLQYAKSC GRYRCRLHHT GLPDFPAIKD GIAQLTYAGP G
	Isoform 2 (E') [UniParc]. Checksum: 27F8B5080EE5E7D7	FASTA	251	27,517
	10 20 30 40 50 60 MGTPWRKRKG IAGPGLPHLS CALVLQPSLR DCLSGLRIEE RAFSYVLTHA LPGDPGHILT 70 80 90 100 110 120 TLDHWSSCCE YLSHMGPVKG QILMRLVEEK APACVLELGT YCGYSTLLIA RALPPGGRLL 130 140 150 160 170 180 TVERDPRTAA VAEKLIRLAG FDEHMVELIV GSSEDVIPCL RTQYQLSRAD LVLLAHRPRC 190 200 210 220 230 240 YLRDLQLLEA HALLPAGATV LADHVLFPGA PRFLQYAKSC GRYRCRLHHT GLPDFPAIKD 250 GIAQLTYAGP G

References

[1]

"Mutations of LRTOMT, a fusion gene with alternative reading frames, cause nonsyndromic deafness in humans." Ahmed Z.M., Masmoudi S., Kalay E., Belyantseva I.A., Mosrati M.A., Collin R.W.J., Riazuddin S., Hmani-Aifa M., Venselaar H., Kawar M.N., Tlili A., van der Zwaag B., Khan S.Y., Ayadi L., Riazuddin S.A., Morell R.J., Griffith A.J., Charfedine I. , Caylan R., Oostrik J., Karaguzel A., Ghorbel A., Riazuddin S., Friedman T.B., Ayadi H., Kremer H. Nat. Genet. 40:1335-1340(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU627074 mRNA. No translation available. EU627075 mRNA. No translation available.
RefSeq	NP_001186282.1. NM_001199353.1.
UniGene	Ptr.6307.
3D structure databases
ProteinModelPortal	P86243.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	748243.
KEGG	ptr:748243.
Organism-specific databases
CTD	220074.
Family and domain databases
InterPro	IPR025782. Catechol_O-MeTrfase. IPR002935. O-MeTrfase_3. [Graphical view]
PANTHER	PTHR10509. PTHR10509. 1 hit.
Pfam	PF01596. Methyltransf_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20914977.

Entry information

Entry name

TOMT_PANTR

Accession

Primary (citable) accession number: P86243

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	October 3, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program