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P86243 (TOMT_PANTR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transmembrane O-methyltransferase

EC=2.1.1.6
Alternative name(s):
Catechol O-methyltransferase 2
Protein LRTOMT2
Gene names
Name:LRTOMT
Synonyms:COMT2, TOMT
OrganismPan troglodytes (Chimpanzee) [Reference proteome]
Taxonomic identifier9598 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePan

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function By similarity. UniProtKB P21964 UniProtKB Q8WZ04

Catalytic activity

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol. UniProtKB P21964

Subcellular location

Isoform 1: Membrane; Single-pass membrane protein Potential.

Isoform 2: Cytoplasm By similarity UniProtKB A1Y9I9.

Miscellaneous

In primates, this protein is produced by a bicistronic gene which also produces the LRRC51 protein from an overlapping reading frame. In rodents, these proteins are produced by 2 separate adjacent genes which together are orthologous to the single primate gene. Ref.1

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: P86243-1)

Also known as: D';

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: P86243-2)

Also known as: E';

The sequence of this isoform differs from the canonical sequence as follows:
     28-67: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Transmembrane O-methyltransferase
PRO_0000372487

Regions

Transmembrane31 – 5121Helical; Potential
Region139 – 1402S-adenosyl-L-methionine binding By similarity

Sites

Binding site1371S-adenosyl-L-methionine By similarity
Binding site1451S-adenosyl-L-methionine By similarity
Binding site1631S-adenosyl-L-methionine By similarity
Binding site1931S-adenosyl-L-methionine; via amide nitrogen By similarity

Natural variations

Alternative sequence28 – 6740Missing in isoform 2. Ref.1
VSP_037158

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (D') [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 171964FA01FA2E67

FASTA29132,124
        10         20         30         40         50         60 
MGTPWRKRKG IAGPGLPHLS CALVLQPRAQ VGTMSPAIAL AFLPLVVTLL VRYRHYFRLL 

        70         80         90        100        110        120 
VRTVLLRSLR DCLSGLRIEE RAFSYVLTHA LPGDPGHILT TLDHWSSCCE YLSHMGPVKG 

       130        140        150        160        170        180 
QILMRLVEEK APACVLELGT YCGYSTLLIA RALPPGGRLL TVERDPRTAA VAEKLIRLAG 

       190        200        210        220        230        240 
FDEHMVELIV GSSEDVIPCL RTQYQLSRAD LVLLAHRPRC YLRDLQLLEA HALLPAGATV 

       250        260        270        280        290 
LADHVLFPGA PRFLQYAKSC GRYRCRLHHT GLPDFPAIKD GIAQLTYAGP G 

« Hide

Isoform 2 (E') [UniParc].

Checksum: 27F8B5080EE5E7D7
Show »

FASTA25127,517

References

[1]"Mutations of LRTOMT, a fusion gene with alternative reading frames, cause nonsyndromic deafness in humans."
Ahmed Z.M., Masmoudi S., Kalay E., Belyantseva I.A., Mosrati M.A., Collin R.W.J., Riazuddin S., Hmani-Aifa M., Venselaar H., Kawar M.N., Tlili A., van der Zwaag B., Khan S.Y., Ayadi L., Riazuddin S.A., Morell R.J., Griffith A.J., Charfedine I. expand/collapse author list , Caylan R., Oostrik J., Karaguzel A., Ghorbel A., Riazuddin S., Friedman T.B., Ayadi H., Kremer H.
Nat. Genet. 40:1335-1340(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU627074 mRNA. No translation available.
EU627075 mRNA. No translation available.
RefSeqNP_001186282.1. NM_001199353.1.
NP_001269004.1. NM_001282075.1.
UniGenePtr.6307.

3D structure databases

ProteinModelPortalP86243.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID748243.
KEGGptr:748243.

Organism-specific databases

CTD220074.

Family and domain databases

InterProIPR025782. Catechol_O-MeTrfase.
IPR002935. O-MeTrfase_3.
[Graphical view]
PANTHERPTHR10509. PTHR10509. 1 hit.
PfamPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PROSITEPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20914977.

Entry information

Entry nameTOMT_PANTR
AccessionPrimary (citable) accession number: P86243
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: February 19, 2014
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families