P86223 (VDAC2_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Voltage-dependent anion-selective channel protein 2
Short name=VDAC-2

Alternative name(s):
Outer mitochondrial membrane protein porin 2

Gene names

Name:

VDAC2

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	122 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective By similarity. UniProtKB P45880
Subunit structure	Interacts with hexokinases By similarity. UniProtKB P45880
Subcellular location	Mitochondrion outer membrane By similarity UniProtKB P45880.
Domain	Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands By similarity. UniProtKB P21796
Sequence similarities	Belongs to the eukaryotic mitochondrial porin family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane beta strand
Ligand	NAD Nucleotide-binding
Molecular function	Porin
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological_process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell pore complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW porin activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›122	›122	Voltage-dependent anion-selective channel protein 2		PRO_0000394307
Regions
	Transmembrane	11 – ›13	›3	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	‹14 – 16	›3	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	21 – ›26	›6	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	‹27 – 34	›8	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	‹41 – 49	›9	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	52 – 59	8	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	63 – 72	10	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	76 – 85	10	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	98 – 107	10	Beta stranded; By similarity UniProtKB P21796
	Transmembrane	110 – 119	10	Beta stranded; By similarity UniProtKB P21796
	Nucleotide binding	98 – 100	3	NAD By similarity UniProtKB P21796
	Nucleotide binding	116 – 120	5	NAD By similarity UniProtKB P21796
Sites
	Site	25	1	Involved in hexokinase binding By similarity UniProtKB P45880
Amino acid modifications
	Modified residue	5	1	N6-acetyllysine By similarity UniProtKB P45880
	Modified residue	13	1	N6-acetyllysine By similarity UniProtKB P45880
	Modified residue	15	1	N6-acetyllysine By similarity UniProtKB P45880
	Modified residue	19	1	Phosphotyrosine By similarity UniProtKB P21796
	Modified residue	34	1	Phosphoserine By similarity UniProtKB P21796
	Modified residue	37	1	Phosphothreonine By similarity UniProtKB P21796
	Modified residue	69	1	Phosphotyrosine By similarity UniProtKB P21796
	Modified residue	122	1	N6-acetyllysine By similarity UniProtKB P21796
Experimental info
	Non-adjacent residues	13 – 14	2
	Non-adjacent residues	26 – 27	2
	Non-adjacent residues	40 – 41	2
	Non-adjacent residues	92 – 93	2
	Non-terminal residue	1	1
	Non-terminal residue	122	1

Sequences

	Sequence		Length	Mass (Da)
	P86223 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: 5547AD7069491C93	FASTA	122	13,420
	10 20 30 40 50 60 DIFNKGFGFG LVKYKWCEYG LTFTEKLTFD TTFSPNTGKK SNFAVGYRTG DFQLHTNVNN 70 80 90 100 110 120 GTEFGGSIYQ KVCEDFDTSV NLAWTSGTNC TRVNNSSLIG VGYTQTLRPG VKLTLSALVD GK

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P86223.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.40.160.10. 2 hits.
InterPro	IPR023614. Porin_dom. IPR027246. Porin_Euk/Tom40. [Graphical view]
Pfam	PF01459. Porin_3. 1 hit. [Graphical view]
PROSITE	PS00558. EUKARYOTIC_PORIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VDAC2_MESAU

Accession

Primary (citable) accession number: P86223

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	May 18, 2010
Last modified:	May 1, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program