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P86219

- ODO2_MESAU

UniProt

P86219 - ODO2_MESAU

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Protein

Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial

Gene

DLST

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity

Catalytic activityi

Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine.By similarity

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191By similaritySequence Analysis
Active sitei123 – 1231By similaritySequence Analysis

GO - Molecular functioni

  1. dihydrolipoyllysine-residue succinyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-lysine catabolic process to acetyl-CoA via saccharopine Source: UniProtKB-UniPathway
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00868; UER00840.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrialBy similarity (EC:2.3.1.61)
Alternative name(s):
2-oxoglutarate dehydrogenase complex component E2By similarity
Short name:
OGDC-E2By similarity
Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complexBy similarity
E2KBy similarity
Gene namesi
Name:DLSTBy similarity
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 145›145Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrialPRO_0000394748Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361N6-acetyllysineBy similarity
Modified residuei66 – 661N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP86219.

Interactioni

Subunit structurei

Forms a 24-polypeptide structural core with octahedral symmetry.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 3128Lipoyl-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the 2-oxoacid dehydrogenase family.Sequence Analysis
Contains 1 lipoyl-binding domain.Sequence Analysis

Keywords - Domaini

Lipoyl

Family and domain databases

Gene3Di3.30.559.10. 2 hits.
InterProiIPR001078. 2-oxoacid_DH_actylTfrase.
IPR023213. CAT-like_dom.
[Graphical view]
PfamiPF00198. 2-oxoacid_dh. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragments.

P86219-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
NDVITVQTPA FAESVTEGDV RVEGGTPLFT LRHNLKLGFM SAFVKASAFA
60 70 80 90 100
LQEQPVVNAV IDDATKEVVY RDYIDISVAV ATPRGLVVPV IRNVETMNYA
110 120 130 140
DIERVEVRPM MYVALTYDHR LIDGREAVTF LRAAVEDPRV LLLDL
Length:145
Mass (Da):16,122
Last modified:June 15, 2010 - v1
Checksum:i32D674B5DD798BBD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-adjacent residuesi21 – 222Curated
Non-adjacent residuesi32 – 332Curated
Non-adjacent residuesi104 – 1052Curated
Non-adjacent residuesi132 – 1332Curated

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P86219.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00868 ; UER00840 .

Family and domain databases

Gene3Di 3.30.559.10. 2 hits.
InterProi IPR001078. 2-oxoacid_DH_actylTfrase.
IPR023213. CAT-like_dom.
[Graphical view ]
Pfami PF00198. 2-oxoacid_dh. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa."
    Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.
    Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiODO2_MESAU
AccessioniPrimary (citable) accession number: P86219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 15, 2010
Last modified: November 26, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3