P86217 (NDUS3_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-30kD
Short name=CI-30kD
NADH-ubiquinone oxidoreductase 30 kDa subunit

Gene names

Name:

NDUFS3

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	94 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. UniProtKB P23709
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. UniProtKB P23709 NADH + acceptor = NAD+ + reduced acceptor. UniProtKB P23709
Subunit structure	Mammalian complex I is composed of 45 different subunits. Interacts with NDUFAF3 By similarity. UniProtKB O75489
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity. UniProtKB Q9DCT2
Sequence similarities	Belongs to the complex I 30 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›94	›94	NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial		PRO_0000394305
Experimental info
	Non-adjacent residues	14 – 15	2
	Non-adjacent residues	28 – 29	2
	Non-adjacent residues	45 – 46	2
	Non-adjacent residues	59 – 60	2
	Non-terminal residue	1	1
	Non-terminal residue	94	1

Sequences

	Sequence		Length	Mass (Da)
	P86217 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: 13BC4321646C6418	FASTA	94	11,107
	10 20 30 40 50 60 VCCRGLLGAA SVGRQNRFEI VYNLLSLREV WDMFGVFFFN HPDLRILTDY GFEGHPFRKY 70 80 90 DDEVKRVVAE PVELAQEFRK FDLNSPWEAF PAYR

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P86217.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001268. NADH_UbQ_OxRdtase_30kDa_su. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. [Graphical view]
PROSITE	PS00542. COMPLEX1_30K. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS3_MESAU

Accession

Primary (citable) accession number: P86217

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	May 18, 2010
Last modified:	October 3, 2012
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program