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Protein

Alpha-enolase

Gene

ENO1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production (By similarity).By similarity

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.By similarity

Cofactori

Mg2+By similarityNote: Binds two Mg2+ per subunit. Required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.By similarity
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Glyceraldehyde-3-phosphate dehydrogenase, testis-specific (GAPDHS), Glyceraldehyde-3-phosphate dehydrogenase (Gapdhs)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Alpha-enolase (ENO1)
  5. Pyruvate kinase (Pklr), Pyruvate kinase (Pkm), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr), Pyruvate kinase (Pklr)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium 1By similarity1
Binding sitei39SubstrateBy similarity1
Active sitei69Proton donorBy similarity1
Metal bindingi99Magnesium 2By similarity1
Metal bindingi119Magnesium 2By similarity1
Binding sitei119SubstrateBy similarity1
Active sitei141Proton acceptorBy similarity1
Binding sitei192SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, Repressor
Biological processGlycolysis, Plasminogen activation, Transcription, Transcription regulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-enolaseBy similarity (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseBy similarity
Enolase 1By similarity
Non-neural enolaseBy similarity
Short name:
NNEBy similarity
Gene namesi
Name:ENO1By similarity
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeMesocricetus
Proteomesi
  • UP000189706 Componenti: Genome assembly

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity

  • Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M-band.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000394395‹1 – 223Alpha-enolaseAdd BLAST›223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphotyrosineBy similarity1
Modified residuei25N6-acetyllysineBy similarity1
Modified residuei61N6-acetyllysineBy similarity1
Modified residuei87N6-acetyllysine; alternateBy similarity1
Modified residuei87N6-malonyllysine; alternateBy similarity1
Modified residuei87N6-succinyllysine; alternateBy similarity1
Modified residuei133N6-acetyllysineBy similarity1
Modified residuei141N6-acetyllysineBy similarity1
Modified residuei215N6-acetyllysine; alternateBy similarity1
Modified residuei215N6-malonyllysine; alternateBy similarity1
Modified residuei215N6-succinyllysine; alternateBy similarity1

Post-translational modificationi

ISGylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiP86210.

Interactioni

Subunit structurei

Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding. Interacts with ENO4 and PGAM2 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP86210.
SMRiP86210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni168 – 171Substrate bindingBy similarity4
Regioni‹202 – 223Required for interaction with PLGBy similarityAdd BLAST›22

Sequence similaritiesi

Belongs to the enolase family.Sequence analysis

Family and domain databases

Gene3Di3.20.20.120. 2 hits.
3.30.390.10. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 2 hits.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Fragments.

P86210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AAVPSGASTG IYEALELRAV EHINKTIAPA LVSKLAMQEF MILPVGASSF
60 70 80 90 100
RIGAEVYHNL KDATNVGDEG GFAPNILENK EALELLKAGY TDQVVIGMDV
110 120 130 140 150
AASEFYRFTA SAGIQVVGDD LTVTNPKRAA SEKSCNCLLL KVNQIGSVTE
160 170 180 190 200
SLQACKLAQS NGWGVMVSHR SGETEDTFIA DLVVGLCTGQ IKTGAPCRSE
210 220
RYNQILRIEE ELGSKSFRNP LAK
Length:223
Mass (Da):23,837
Last modified:May 18, 2010 - v1
Checksum:i06EB554BA921BA8C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Non-adjacent residuesi18 – 19Curated2
Non-adjacent residuesi34 – 35Curated2
Non-adjacent residuesi51 – 52Curated2
Non-adjacent residuesi87 – 88Curated2
Non-adjacent residuesi107 – 108Curated2
Sequence uncertaintyi111S or T1
Non-adjacent residuesi128 – 129Curated2
Non-adjacent residuesi201 – 202Curated2
Non-adjacent residuesi215 – 216Curated2

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENOA_MESAU
AccessioniPrimary (citable) accession number: P86210
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: July 5, 2017
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families