P86203 (NDUS1_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-75kD
Short name=CI-75kD

Gene names

Name:

NDUFS1

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	190 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity. UniProtKB P15690
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. UniProtKB P15690 NADH + acceptor = NAD+ + reduced acceptor. UniProtKB P15690
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. UniProtKB P29915 Binds 2 4Fe-4S clusters per subunit By similarity. UniProtKB P29915
Subunit structure	Complex I is composed of 45 different subunits By similarity. UniProtKB P15690
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity. UniProtKB Q91VD9
Sequence similarities	Belongs to the complex I 75 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›190	›190	NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial		PRO_0000394295
Experimental info
	Non-adjacent residues	16 – 17	2
	Non-adjacent residues	36 – 37	2
	Non-adjacent residues	44 – 45	2
	Non-adjacent residues	53 – 54	2
	Non-adjacent residues	67 – 68	2
	Non-adjacent residues	76 – 77	2
	Non-adjacent residues	98 – 99	2
	Non-adjacent residues	137 – 138	2
	Non-adjacent residues	147 – 148	2
	Non-adjacent residues	161 – 162	2
	Non-adjacent residues	180 – 181	2
	Non-terminal residue	1	1
	Non-terminal residue	190	1

Sequences

	Sequence		Length	Mass (Da)
	P86203 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: B3DC03A97075FA9C	FASTA	190	20,736
	10 20 30 40 50 60 FASEIAGVDD LGTTGRKTES IDVMDAVGSN IVVSTRFAYD GLKRQRLTEP MVRGLLTYTS 70 80 90 100 110 120 WEDALSRFEA PLFNARVALI GSPVDLTYRY DHLGDSPKIA SQVAALDLGY KPGVEAIRKN 130 140 150 160 170 180 PPKLLFLLGA DGGCITRSAT YVNTEGRVAV TPPGLAREDW KALSEIAGIT LPYDTLDQVR 190 DFYMTDSISR

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P86203.
ProMEX	P86203.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. Partial match. PS00641. COMPLEX1_75K_1. Partial match. PS00642. COMPLEX1_75K_2. Partial match. PS00643. COMPLEX1_75K_3. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_MESAU

Accession

Primary (citable) accession number: P86203

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	May 18, 2010
Last modified:	April 3, 2013
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program