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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Gene

NDUFS1

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.By similarity

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialBy similarity (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kDBy similarity
Short name:
CI-75kDBy similarity
Gene namesi
Name:NDUFS1By similarity
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

  1. Mitochondrion inner membrane By similarity

  2. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›190›190NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrialPRO_0000394295Add
BLAST

Proteomic databases

PRIDEiP86203.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits.By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Sequence Analysis

Sequencei

Sequence statusi: Fragments.

P86203-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FASEIAGVDD LGTTGRKTES IDVMDAVGSN IVVSTRFAYD GLKRQRLTEP
60 70 80 90 100
MVRGLLTYTS WEDALSRFEA PLFNARVALI GSPVDLTYRY DHLGDSPKIA
110 120 130 140 150
SQVAALDLGY KPGVEAIRKN PPKLLFLLGA DGGCITRSAT YVNTEGRVAV
160 170 180 190
TPPGLAREDW KALSEIAGIT LPYDTLDQVR DFYMTDSISR
Length:190
Mass (Da):20,736
Last modified:May 18, 2010 - v1
Checksum:iB3DC03A97075FA9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-adjacent residuesi16 – 172Curated
Non-adjacent residuesi36 – 372Curated
Non-adjacent residuesi44 – 452Curated
Non-adjacent residuesi53 – 542Curated
Non-adjacent residuesi67 – 682Curated
Non-adjacent residuesi76 – 772Curated
Non-adjacent residuesi98 – 992Curated
Non-adjacent residuesi137 – 1382Curated
Non-adjacent residuesi147 – 1482Curated
Non-adjacent residuesi161 – 1622Curated
Non-adjacent residuesi180 – 1812Curated
Non-terminal residuei190 – 1901

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP86203.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa."
    Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.
    Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiNDUS1_MESAU
AccessioniPrimary (citable) accession number: P86203
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: May 18, 2010
Last modified: January 7, 2015
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.