P86200 (NAGK_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
Short name=N-acetylglucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:

NAGK

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	61 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway. Also has ManNAc kinase activity By similarity. UniProtKB Q9QZ08
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. UniProtKB Q9QZ08
Pathway	Amino-sugar metabolism; N-acetylneuraminate degradation.
Subunit structure	Homodimer By similarity. UniProtKB Q9UJ70
Sequence similarities	Belongs to the eukaryotic-type N-acetylglucosamine kinase family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	N-acetylneuraminate catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW N-acetylglucosamine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›61	›61	N-acetyl-D-glucosamine kinase		PRO_0000394398
Sites
	Binding site	45	1	ATP By similarity UniProtKB Q9UJ70
Amino acid modifications
	Modified residue	30	1	Phosphotyrosine By similarity UniProtKB Q9UJ70
Experimental info
	Non-adjacent residues	10 – 11	2
	Non-adjacent residues	31 – 32	2
	Non-adjacent residues	44 – 45	2
	Non-terminal residue	1	1
	Non-terminal residue	61	1

Sequences

	Sequence		Length	Mass (Da)
	P86200 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: F24D4EE847EBDF63	FASTA	61	6,868
	10 20 30 40 50 60 AGVDPLVPLR QAMFNYFQVP DRLGILTHLY RIAEGAQQGD PLSRSWELLK EGFLLALTQG R

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P86200.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00629.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

NAGK_MESAU

Accession

Primary (citable) accession number: P86200

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	February 6, 2013
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program