P86198 (BDH_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-beta-hydroxybutyrate dehydrogenase, mitochondrial
Short name=BDH
EC=1.1.1.30

Alternative name(s):
3-hydroxybutyrate dehydrogenase

Gene names

Name:	BDH1
Synonyms:	BDH

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	132 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH. UniProtKB Q02338
Enzyme regulation	Requires phosphatidylcholine as an allosteric activator for enzymatic activity By similarity. UniProtKB Q02338
Subunit structure	Homotetramer By similarity. UniProtKB Q02338
Subcellular location	Mitochondrion matrix By similarity UniProtKB Q02338.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-hydroxybutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›132	›132	D-beta-hydroxybutyrate dehydrogenase, mitochondrial		PRO_0000394393
Regions
	Nucleotide binding	3 – 27	25	NAD By similarity UniProtKB Q02338
Sites
	Active site	66	1	Proton acceptor By similarity UniProtKB Q02338
	Binding site	59	1	Substrate By similarity UniProtKB Q02338
Experimental info
	Non-adjacent residues	33 – 34	2
	Non-adjacent residues	45 – 46	2
	Non-adjacent residues	53 – 54	2
	Non-adjacent residues	63 – 64	2
	Non-adjacent residues	110 – 111	2
	Non-adjacent residues	121 – 122	2
	Non-terminal residue	1	1
	Non-terminal residue	132	1

Sequences

	Sequence		Length	Mass (Da)
	P86198 [UniParc]. Last modified May 18, 2010. Version 1. Checksum: 5BEE8D8BF102E584	FASTA	132	15,007
	10 20 30 40 50 60 AVLVTGCDSG FGFSLAKHLH SKGFLVFAGC LLKEVAEVNL WGTVRSFLPL LRRVVNISSM 70 80 90 100 110 120 LGRSPYCITK FGVEAFSDCL RYEMHPLGVK VSVVEPGNFI AATSLYSPER MWDELPEVVR 130 KYHPMDYYWW LR

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

3D structure databases
ProteinModelPortal	P86198.
ModBase	Search...
Proteomic databases
PRIDE	P86198.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR016040. NAD(P)-bd_dom. [Graphical view]
PROSITE	PS00061. ADH_SHORT. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BDH_MESAU

Accession

Primary (citable) accession number: P86198

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 18, 2010
Last sequence update:	May 18, 2010
Last modified:	March 6, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program