P86197 (ODP2_MESAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
EC=2.3.1.12

Alternative name(s):
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Pyruvate dehydrogenase complex component E2
Short name=PDC-E2
Short name=PDCE2

Gene names

Name:

DLAT

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	219 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle By similarity. UniProtKB P08461
Catalytic activity	Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine. UniProtKB P08461
Cofactor	Binds 1 lipoyl cofactor covalently By similarity. UniProtKB P08461
Subunit structure	Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules. Interacts with PDK2 and PDK3 By similarity.
Subcellular location	Mitochondrion matrix By similarity UniProtKB P08461.
Tissue specificity	Detected at higher levels in cauda epididymal spermatazoa than in caput epididymal spermatazoa (at protein level). Ref.1
Sequence similarities	Belongs to the 2-oxoacid dehydrogenase family. Contains 2 lipoyl-binding domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Lipoyl Repeat
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological_process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dihydrolipoyllysine-residue acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 219	›219	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial		PRO_0000394399
Regions
	Domain	‹1 – ›17	›17	Lipoyl-binding 1 UniProtKB P08461
	Domain	‹18 – 28	›11	Lipoyl-binding 2 UniProtKB P08461
	Region	37 – 57	21	E3- and/or E1-component binding domain Potential UniProtKB P08461
	Region	187 – 198	12	CoA-binding By similarity UniProtKB P08461
Sites
	Active site	192	1	Potential UniProtKB P08461
	Active site	196	1	Potential UniProtKB P08461
Experimental info
	Non-adjacent residues	17 – 18	2
	Non-adjacent residues	31 – 32	2
	Non-adjacent residues	40 – 41	2
	Non-adjacent residues	55 – 56	2
	Non-adjacent residues	63 – 64	2
	Non-adjacent residues	121 – 122	2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P86197 [UniParc]. Last modified June 15, 2010. Version 1. Checksum: 11B4C4C826FE3B08	FASTA	219	23,117
	10 20 30 40 50 60 VPLPSLSPTM QAGTIARDVP LGAPLCIIVE KGRVFVSPLA KGIDLTQVKG TGPEGDIDSF 70 80 90 100 110 120 VPSKVPEANS SWMDTVIRQN HVVDVSVAVS TPAGLITPIV FNAHIKGLET IASDVVSLAS 130 140 150 160 170 180 KEGKLQPHEF QGGTFTISNL GMFGIKNFSA IINPPQACIL AIGASEDKLI PADNEKGFDV 190 200 210 ASVMSVTLSC DHRVVDGAVG AQWLAEFKKY LEKPITMLL

References

[1]

"Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa." Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S. Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.

Cross-references

3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P86197.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.30.559.10. 1 hit. 4.10.320.10. 1 hit.
InterPro	IPR001078. 2-oxoacid_DH_actylTfrase. IPR023213. CAT-like_dom. IPR004167. E3-bd. [Graphical view]
Pfam	PF00198. 2-oxoacid_dh. 1 hit. [Graphical view]
PROSITE	PS50968. BIOTINYL_LIPOYL. Partial match. PS00189. LIPOYL. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ODP2_MESAU

Accession

Primary (citable) accession number: P86197

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	April 3, 2013
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program