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P86197

- ODP2_MESAU

UniProt

P86197 - ODP2_MESAU

Protein

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial

Gene

DLAT

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 17 (01 Oct 2014)
      Sequence version 1 (15 Jun 2010)
      Previous versions | rss
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    Functioni

    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.By similarity

    Catalytic activityi

    Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine.By similarity

    Cofactori

    Binds 1 lipoyl cofactor covalently.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei192 – 1921By similaritySequence Analysis
    Active sitei196 – 1961By similaritySequence Analysis

    GO - Molecular functioni

    1. dihydrolipoyllysine-residue acetyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Tricarboxylic acid cycle

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrialBy similarity (EC:2.3.1.12)
    Alternative name(s):
    Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complexBy similarity
    Pyruvate dehydrogenase complex component E2By similarity
    Short name:
    PDC-E2By similarity
    Short name:
    PDCE2By similarity
    Gene namesi
    Name:DLATBy similarity
    OrganismiMesocricetus auratus (Golden hamster)
    Taxonomic identifieri10036 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 219›219Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrialPRO_0000394399Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei121 – 1211N6-succinyllysineBy similarity

    Proteomic databases

    PRIDEiP86197.

    Expressioni

    Tissue specificityi

    Detected at higher levels in cauda epididymal spermatazoa than in caput epididymal spermatazoa (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Part of the multimeric pyruvate dehydrogenase complex that contains multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). These subunits are bound to an inner core composed of about 48 DLAT and 12 PDHX molecules. Interacts with PDK2 and PDK3 By similarity.By similarity

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini‹1 – ›17›17Lipoyl-binding 1By similarityAdd
    BLAST
    Domaini‹18 – 28›11Lipoyl-binding 2By similarityAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni37 – 5721E3- and/or E1-component binding domainBy similaritySequence AnalysisAdd
    BLAST
    Regioni187 – 19812CoA-bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the 2-oxoacid dehydrogenase family.Sequence Analysis
    Contains 2 lipoyl-binding domains.Sequence Analysis

    Keywords - Domaini

    Lipoyl, Repeat

    Family and domain databases

    Gene3Di3.30.559.10. 1 hit.
    4.10.320.10. 1 hit.
    InterProiIPR001078. 2-oxoacid_DH_actylTfrase.
    IPR023213. CAT-like_dom.
    IPR004167. E3-bd.
    [Graphical view]
    PfamiPF00198. 2-oxoacid_dh. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragments.

    P86197-1 [UniParc]FASTAAdd to Basket

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    VPLPSLSPTM QAGTIARDVP LGAPLCIIVE KGRVFVSPLA KGIDLTQVKG    50
    TGPEGDIDSF VPSKVPEANS SWMDTVIRQN HVVDVSVAVS TPAGLITPIV 100
    FNAHIKGLET IASDVVSLAS KEGKLQPHEF QGGTFTISNL GMFGIKNFSA 150
    IINPPQACIL AIGASEDKLI PADNEKGFDV ASVMSVTLSC DHRVVDGAVG 200
    AQWLAEFKKY LEKPITMLL 219
    Length:219
    Mass (Da):23,117
    Last modified:June 15, 2010 - v1
    Checksum:i11B4C4C826FE3B08
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-adjacent residuesi17 – 182Curated
    Non-adjacent residuesi31 – 322Curated
    Non-adjacent residuesi40 – 412Curated
    Non-adjacent residuesi55 – 562Curated
    Non-adjacent residuesi63 – 642Curated
    Non-adjacent residuesi121 – 1222Curated

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P86197.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.559.10. 1 hit.
    4.10.320.10. 1 hit.
    InterProi IPR001078. 2-oxoacid_DH_actylTfrase.
    IPR023213. CAT-like_dom.
    IPR004167. E3-bd.
    [Graphical view ]
    Pfami PF00198. 2-oxoacid_dh. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa."
      Kameshwari D.B., Bhande S., Sundaram C.S., Kota V., Siva A.B., Shivaji S.
      Asian J. Androl. 12:344-355(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiODP2_MESAU
    AccessioniPrimary (citable) accession number: P86197
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: June 15, 2010
    Last modified: October 1, 2014
    This is version 17 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3