P86144 (RIPD1_PHYDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Dioicin-1 EC=3.2.2.22 Alternative name(s): Ribosome-inactivating protein rRNA N-glycosidase
Organism	Phytolacca dioica (Bella sombra tree) (Phytolacca arborea)
Taxonomic identifier	29725 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Phytolaccaceae › Phytolacca

Protein attributes

Sequence length	22 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nicks pBR322 dsDNA. Has adenine polynucleotide glycosidase activity on herring sperm ssDNA. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. Ref.1
Subcellular location	Secreted › extracellular space. Golgi apparatus. Vacuole Ref.1.
Developmental stage	Detected in fully expanded leaves of 8 to 34 month old plants, levels peak in autumn. Also present in developing leaves of adult plants, detected in 10 and 17 day old leaves but not in 25, 40 or 60 day old leaves. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Mass spectrometry	Molecular mass is 30047.00 Da from positions 1 - ?. Determined by ESI. Ref.1

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Golgi apparatus Secreted Vacuole
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›22	›22	Dioicin-1		PRO_0000372679
Experimental info
	Non-terminal residue	22	1

Sequences

	Sequence		Length	Mass (Da)
	P86144 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: A565D20AFDC99888	FASTA	22	2,372
	10 20 ANIVFDVESA TTGTYSTFLT SF

References

[1]

"Type 1 ribosome-inactivating proteins from Phytolacca dioica L. leaves: differential seasonal and age expression, and cellular localization." Parente A., Conforto B., Di Maro A., Chambery A., De Luca P., Bolognesi A., Iriti M., Faoro F. Planta 228:963-975(2008) [PubMed: 18704492] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, MASS SPECTROMETRY. Tissue: Leaf.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIPD1_PHYDI

Accession

Primary (citable) accession number: P86144

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	May 31, 2011
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program