P86137 (NLTP1_ACTDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-specific lipid-transfer protein 1 Short name=LTP1 Short name=nsLTP1 Alternative name(s): Allergen=Act d 10.01
Organism	Actinidia deliciosa (Kiwi)
Taxonomic identifier	3627 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › Ericales › Actinidiaceae › Actinidia

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.
Tissue specificity	Expressed in seeds and, at very low levels, in pulp of fruit (at protein level). Ref.1
Allergenic properties	Causes an allergic reaction in human. Ref.1
Sequence similarities	Belongs to the plant LTP family.
Mass spectrometry	Molecular mass is 9464±20 Da from positions 1 - 92. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Transport
Disease	Allergen
Ligand	Lipid-binding
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid transport Inferred from electronic annotation. Source: InterPro
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 92	92	Non-specific lipid-transfer protein 1		PRO_0000392467
Amino acid modifications
	Disulfide bond	4 ↔ 52		By similarity UniProtKB Q42952
	Disulfide bond	14 ↔ 28		By similarity UniProtKB Q42952
	Disulfide bond	29 ↔ 74		By similarity UniProtKB Q42952
	Disulfide bond	50 ↔ 88		By similarity UniProtKB Q42952

Sequences

	Sequence		Length	Mass (Da)
	P86137 [UniParc]. Last modified February 22, 2012. Version 2. Checksum: A585FB1572EFE186	FASTA	92	9,458
	10 20 30 40 50 60 AVSCGQVDTA LTPCLTYLTK GGTPSTQCCS GVRSLKSMTG TKVPDRQAAC NCLKQAAARY 70 80 90 QGIKDAAAAL SQKCGVQLSV PISRSTDCSK IS

References

[1]

"Allergenic lipid transfer proteins from plant-derived foods do not immunologically and clinically behave homogeneously: the kiwifruit LTP as a model." Bernardi M.L., Giangrieco I., Camardella L., Ferrara R., Palazzo P., Panico M.R., Crescenzo R., Carratore V., Zennaro D., Liso M., Santoro M., Zuzzi S., Tamburrini M., Ciardiello M.A., Mari A. PLoS ONE 6:E27856-E27856(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, TISSUE SPECIFICITY, MASS SPECTROMETRY, ALLERGENICITY. Tissue: Seed.

[2]

"Biochemical and immunological characterization of non-specific lipid transfer protein (nsLTP) from kiwi fruit." Bublin M., Radauer C., Breiteneder H. Submitted (NOV-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-13. Strain: cv. Hayward. Tissue: Fruit.

Cross-references

3D structure databases
ModBase	Search...
Protein family/group databases
Allergome	5737. Act d 10. 5738. Act d 10.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016140. Bifunc_inhib/LTP/seed_store. IPR000528. Plant_LTP. [Graphical view]
Pfam	PF00234. Tryp_alpha_amyl. 1 hit. [Graphical view]
PRINTS	PR00382. LIPIDTRNSFER.
SMART	SM00499. AAI. 1 hit. [Graphical view]
SUPFAM	SSF47699. Bifunc_inhib/LTP/seed_store. 1 hit.
PROSITE	PS00597. PLANT_LTP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NLTP1_ACTDE

Accession

Primary (citable) accession number: P86137
Secondary accession number(s): P85205

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	February 22, 2012
Last modified:	March 6, 2013
This is version 8 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program