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P86110 (KKX25_OPICY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 10. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin kappa-KTx 2.5
Alternative name(s):
OcyC8
OcyKTx6
OrganismOpisthacanthus cayaporum (South American scorpion)
Taxonomic identifier573324 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaScorpionoideaHemiscorpiidaeOpisthacanthus

Protein attributes

Sequence length70 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Voltage-independently blocks potassium currents on human Kv1.1/KCNA1 (IC50=217 µM), and human Kv1.4/KCNA4 (IC50=71 µM) (expressed in CHO cells). Ref.2

Subcellular location

Secreted Ref.2 Ref.3.

Tissue specificity

Expressed by the venom gland. Ref.2 Ref.3

Miscellaneous

Has no effect on other potassium channels, on sodium channels, on bacterial growth and on smooth muscle tissue (a known assay to identify possible bradykinin-potentiating peptides) (Ref.2).

Sequence similarities

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor kappa-KTx family. Kappa-KTx 2 subfamily.

Mass spectrometry

Molecular mass is 3132.26 Da from positions 43 - 70. Determined by MALDI. Monoisotopic mass. Ref.2

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Propeptide27 – 4216
PRO_0000398603
Peptide43 – 7028Potassium channel toxin kappa-KTx 2.5 Ref.2
PRO_0000398136

Amino acid modifications

Disulfide bond46 ↔ 64 By similarity
Disulfide bond50 ↔ 60 By similarity

Experimental info

Sequence conflict531H → N AA sequence Ref.3

Sequences

Sequence LengthMass (Da)Tools
P86110 [UniParc].

Last modified October 5, 2010. Version 1.
Checksum: 152071A7A97408C5

FASTA707,823
        10         20         30         40         50         60 
MESSRKSYVL MLFLAFVIMN VCSVSGEPKD GEIAGFEMEE ARYDACVNAC LEHHPNVREC 

        70 
EEACKNPVPP 

« Hide

References

[1]"Cloning and characterization of cDNA sequences encoding for new venom peptides of the Brazilian scorpion Opisthacanthus cayaporum."
Silva E.C., Camargos T.S., Maranhao A.Q., Silva-Pereira I., Silva L.P., Possani L.D., Schwartz E.F.
Toxicon 54:252-261(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Venom gland.
[2]"The new kappa-KTx 2.5 from the scorpion Opisthacanthus cayaporum."
Camargos T.S., Restano-Cassulini R., Possani L.D., Peigneur S., Tytgat J., Schwartz C.A., Alves E.M., de Freitas S.M., Schwartz E.F.
Peptides 32:1509-1517(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 43-70, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, SYNTHESIS OF 43-70, DOMAIN, CIRCULAR DICHROISM, MASS SPECTROMETRY.
Tissue: Venom.
[3]"Mass spectrometry analysis, amino acid sequence and biological activity of venom components from the Brazilian scorpion Opisthacanthus cayaporum."
Schwartz E.F., Camargos T.S., Zamudio F.Z., Silva L.P., Bloch C. Jr., Caixeta F., Schwartz C.A., Possani L.D.
Toxicon 51:1499-1508(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 43-64, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Venom.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FM998750 mRNA. Translation: CAX51396.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameKKX25_OPICY
AccessionPrimary (citable) accession number: P86110
Secondary accession number(s): C5J892
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: October 5, 2010
Last modified: April 16, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries