P86102 (E13B_VITRO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase Short name=(1->3)-beta-glucanase Beta-1,3-endoglucanase
Organism	Vitis rotundifolia (Muscadine grape)
Taxonomic identifier	103349 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › rosids incertae sedis › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	34 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans. UniProtKB Q03773
Miscellaneous	On the 2D-gel the determined pI of this protein is: 6, its MW is: 25 kDa. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›34	›34	Glucan endo-1,3-beta-glucosidase		PRO_0000358869
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	34	1

Sequences

	Sequence		Length	Mass (Da)
	P86102 [UniParc]. Last modified December 16, 2008. Version 1. Checksum: F58BE59A84C58419	FASTA	34	3,406
	10 20 30 NIFNAISAAG LGNQIKVSTA IDTGVLGTSY PPSK

References

[1]	"Proteomics approach to identify unique xylem sap proteins in Pierce's disease-tolerant Vitis species." Basha S.M., Mazhar H., Vasanthaiah H.K.N. Appl. Biochem. Biotechnol. 160:932-944(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Xylem.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_VITRO

Accession

Primary (citable) accession number: P86102

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	May 1, 2013
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program