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P86100

- HYAL1_MESMA

UniProt

P86100 - HYAL1_MESMA

Protein

Hyaluronidase-1

Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 8 (01 Oct 2014)
      Sequence version 2 (19 Mar 2014)
      Previous versions | rss
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    Functioni

    Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. In venom, it may participate in the degradation of the extracellular matrix thus allowing a rapid spread of other venom components during the envenomation process.1 Publication

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

    Enzyme regulationi

    Inhibited by heparin, and to a lesser extent by DTT, Fe3+ and Cu2+. Not inhibited by reduced glutathione, L-cysteine, EDTA, Ca2+ or Mg2+.1 Publication

    pH dependencei

    Optimum pH is 4.5.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius. Activity decreases when incubated at temperatures above 24 degrees Celsius for 10 minutes. No activity remains after 10 minutes at 65 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei130 – 1301Proton donorBy similarity

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB

    GO - Biological processi

    1. pathogenesis Source: UniProtKB
    2. polysaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase-11 Publication (EC:3.2.1.35)
    Short name:
    BmHYA1
    Short name:
    HYA11 Publication
    Alternative name(s):
    Hyaluronoglucosaminidase-1
    Venom spreading factor
    OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
    Taxonomic identifieri34649 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 25251 PublicationAdd
    BLAST
    Chaini26 – 410385Hyaluronidase-1PRO_0000355081Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi41 ↔ 331PROSITE-ProRule annotation
    Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi111 – 1111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi199 ↔ 242PROSITE-ProRule annotation
    Disulfide bondi206 ↔ 220PROSITE-ProRule annotation
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi356 ↔ 367PROSITE-ProRule annotation
    Disulfide bondi361 ↔ 395PROSITE-ProRule annotation
    Glycosylationi392 – 3921N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi397 ↔ 406PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini352 – 40756EGF-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.1 Publication
    Contains 1 EGF-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Signal

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
    PRINTSiPR00846. GLHYDRLASE56.
    SMARTiSM00181. EGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P86100-1 [UniParc]FASTAAdd to Basket

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    MTQNIQMTEM YQIILFASIL AAISATSADF KVVWEVPSIM CSKKFKINVT    50
    DLLTSHKILV NQEETFNGDK IVIFYESQLG KYPHIESHGD INGGMLQVSD 100
    LANHLKIARD NISKFIPDPN FNGVGIIDWE AWRPLWKYNW GRMSEYRDRS 150
    KDLVKAKHPD WSPAQIEKVA IEEWENSAKE WMLKTLKLVE DMRPNAAWCY 200
    YLFPDCYNYG GKDQPSEYFC KNDIQEANDK LSWLWKQSTA LCPSIYMQES 250
    HITKYNTSQR AWWIYARLRE TIRLSHPNTL IYPYINYILP GTKKTVPSMD 300
    FKRVLGQIGS LGLDGAIIWG SSYHVNTEEM CKEMKTYVKD VIAPVASTVI 350
    QNVNRCSQQI CKGRGNCVWP EEPYTSWKYL IDPKNPTFKH TNISCKCKGG 400
    YTGRYCQIAP 410
    Length:410
    Mass (Da):47,433
    Last modified:March 19, 2014 - v2
    Checksum:i4472C474E9E4DF56
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481N → C AA sequence (PubMed:18611448)Curated
    Sequence conflicti102 – 1021A → T no nucleotide entry (PubMed:20417653)Curated
    Sequence conflicti132 – 1321W → G no nucleotide entry (PubMed:20417653)Curated
    Sequence conflicti135 – 1351L → F no nucleotide entry (PubMed:20417653)Curated
    Sequence conflicti189 – 1891V → A in ACY69673. (PubMed:20417653)Curated
    Sequence conflicti235 – 2351W → G no nucleotide entry (PubMed:20417653)Curated
    Sequence conflicti329 – 3291E → G in ACY69673. (PubMed:20417653)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    KF588514 mRNA. Translation: AHA36327.1.
    GU130249 mRNA. Translation: ACY69673.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    KF588514 mRNA. Translation: AHA36327.1 .
    GU130249 mRNA. Translation: ACY69673.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000742. EG-like_dom.
    IPR013032. EGF-like_CS.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
    PRINTSi PR00846. GLHYDRLASE56.
    SMARTi SM00181. EGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    PS01186. EGF_2. 1 hit.
    PS50026. EGF_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and molecular characterization of scorpion Buthus martensi venom hyaluronidases: a novel full-length and diversiform no-code stop sequences."
      Xue S., Zhao Y., Ma Y., Gui G., Huang C.
      Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and molecular characterization of BmHYA1, a novel hyaluronidase from the venom of Chinese red scorpion Buthus martensi Karsch."
      Feng L., Gao R., Meng J., Gopalakrishnakone P.
      Toxicon 56:474-479(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-410.
    3. "Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi."
      Feng L., Gao R., Gopalakrishnakone P.
      Comp. Biochem. Physiol. 148C:250-257(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 26-55, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Venom1 Publication.

    Entry informationi

    Entry nameiHYAL1_MESMA
    AccessioniPrimary (citable) accession number: P86100
    Secondary accession number(s): D1MBU1, U6BKM8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: March 19, 2014
    Last modified: October 1, 2014
    This is version 8 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3