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P86100 (HYAL1_MESMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hyaluronidase-1

Short name=BmHYA1
Short name=HYA1
EC=3.2.1.35
Alternative name(s):
Hyaluronoglucosaminidase-1
Venom spreading factor
OrganismMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifier34649 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. In venom, it may participate in the degradation of the extracellular matrix thus allowing a rapid spread of other venom components during the envenomation process. Ref.3

Catalytic activity

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.3

Enzyme regulation

Inhibited by heparin, and to a lesser extent by DTT, Fe3+ and Cu2+. Not inhibited by reduced glutathione, L-cysteine, EDTA, Ca2+ or Mg2+. Ref.3

Subcellular location

Secreted Ref.3.

Tissue specificity

Expressed by the venom gland. Ref.3

Sequence similarities

Belongs to the glycosyl hydrolase 56 family. Ref.3

Contains 1 EGF-like domain.

Biophysicochemical properties

pH dependence:

Optimum pH is 4.5. Ref.3

Temperature dependence:

Optimum temperature is 50 degrees Celsius. Activity decreases when incubated at temperatures above 24 degrees Celsius for 10 minutes. No activity remains after 10 minutes at 65 degrees Celsius. Ref.3

Ontologies

Keywords
   Cellular componentSecreted
   DomainEGF-like domain
Signal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from direct assay Ref.3. Source: UniProtKB

polysaccharide catabolic process

Inferred from direct assay Ref.3. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionhyalurononglucosaminidase activity

Inferred from direct assay Ref.3. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Ref.3
Chain26 – 410385Hyaluronidase-1
PRO_0000355081

Regions

Domain352 – 40756EGF-like

Sites

Active site1301Proton donor By similarity

Amino acid modifications

Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation1111N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation3921N-linked (GlcNAc...) Potential
Disulfide bond41 ↔ 331 By similarity
Disulfide bond199 ↔ 242 By similarity
Disulfide bond206 ↔ 220 By similarity
Disulfide bond356 ↔ 367 By similarity
Disulfide bond361 ↔ 395 By similarity
Disulfide bond397 ↔ 406 By similarity

Experimental info

Sequence conflict481N → C AA sequence Ref.3
Sequence conflict1021A → T no nucleotide entry Ref.2
Sequence conflict1321W → G no nucleotide entry Ref.2
Sequence conflict1351L → F no nucleotide entry Ref.2
Sequence conflict1891V → A in ACY69673. Ref.2
Sequence conflict2351W → G no nucleotide entry Ref.2
Sequence conflict3291E → G in ACY69673. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P86100 [UniParc].

Last modified March 19, 2014. Version 2.
Checksum: 4472C474E9E4DF56

FASTA41047,433
        10         20         30         40         50         60 
MTQNIQMTEM YQIILFASIL AAISATSADF KVVWEVPSIM CSKKFKINVT DLLTSHKILV 

        70         80         90        100        110        120 
NQEETFNGDK IVIFYESQLG KYPHIESHGD INGGMLQVSD LANHLKIARD NISKFIPDPN 

       130        140        150        160        170        180 
FNGVGIIDWE AWRPLWKYNW GRMSEYRDRS KDLVKAKHPD WSPAQIEKVA IEEWENSAKE 

       190        200        210        220        230        240 
WMLKTLKLVE DMRPNAAWCY YLFPDCYNYG GKDQPSEYFC KNDIQEANDK LSWLWKQSTA 

       250        260        270        280        290        300 
LCPSIYMQES HITKYNTSQR AWWIYARLRE TIRLSHPNTL IYPYINYILP GTKKTVPSMD 

       310        320        330        340        350        360 
FKRVLGQIGS LGLDGAIIWG SSYHVNTEEM CKEMKTYVKD VIAPVASTVI QNVNRCSQQI 

       370        380        390        400        410 
CKGRGNCVWP EEPYTSWKYL IDPKNPTFKH TNISCKCKGG YTGRYCQIAP 

« Hide

References

[1]"Cloning and molecular characterization of scorpion Buthus martensi venom hyaluronidases: a novel full-length and diversiform no-code stop sequences."
Xue S., Zhao Y., Ma Y., Gui G., Huang C.
Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and molecular characterization of BmHYA1, a novel hyaluronidase from the venom of Chinese red scorpion Buthus martensi Karsch."
Feng L., Gao R., Meng J., Gopalakrishnakone P.
Toxicon 56:474-479(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-410.
[3]"Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi."
Feng L., Gao R., Gopalakrishnakone P.
Comp. Biochem. Physiol. 148C:250-257(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-55, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Venom.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
KF588514 mRNA. Translation: AHA36327.1.
GU130249 mRNA. Translation: ACY69673.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERPTHR11769. PTHR11769. 1 hit.
PfamPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFPIRSF038193. Hyaluronidase. 1 hit.
PRINTSPR00846. GLHYDRLASE56.
SMARTSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHYAL1_MESMA
AccessionPrimary (citable) accession number: P86100
Secondary accession number(s): D1MBU1, U6BKM8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 19, 2014
Last modified: May 14, 2014
This is version 7 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries