P86100 (HYAL1_MESMA) Reviewed, UniProtKB/Swiss-Prot
Last modified
March 3, 2009.
Version 3.
History...
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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Hyaluronidase-1 Short name=HYA1 EC=3.2.1.35 Alternative name(s): Hyaluronoglucosaminidase-1 |
| Organism | Mesobuthus martensii (Manchurian scorpion) (Buthus martensii) |
| Taxonomic identifier | 34649 [NCBI] |
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus |
Protein attributes
| Sequence length | 30 AA. |
| Sequence status | Fragment. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. Ref.1 |
| Catalytic activity | Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.1 |
| Enzyme regulation | Inhibited by heparin, and to a lesser extent by DTT, Fe3+ and Cu2+. Not inhibited by reduced glutathione, L-cysteine, EDTA, Ca2+ or Mg2+. Ref.1 |
| Subcellular location | |
| Tissue specificity | Expressed by the venom gland. Ref.1 |
| Sequence similarities | Belongs to the glycosyl hydrolase 56 family. Ref.1 |
| Biophysicochemical properties | pH dependence: Optimum pH is 4.5. Ref.1 Temperature dependence: Optimum temperature is 50 degrees Celsius. Activity decreases when incubated at temperatures above 24 degrees Celsius for 10 minutes. No activity remains after 10 minutes at 65 degrees Celsius. Ref.1 |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Secreted |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | pathogenesis Inferred from direct assay Ref.1. Source: UniProtKB polysaccharide catabolic processInferred from direct assay Ref.1. Source: UniProtKB |
| Cellular component | extracellular region Inferred from direct assay Ref.1. Source: UniProtKB |
| Molecular function | hyalurononglucosaminidase activity Inferred from direct assay Ref.1. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||
Molecule processing | ||||||||
|---|---|---|---|---|---|---|---|---|
| Chain | 1 – ›30 | ›30 | Hyaluronidase-1 | PRO_0000355081 | ||||
Amino acid modifications | ||||||||
| Disulfide bond | 16 ↔ ? | By similarity UniProtKB P85841 | ||||||
Experimental info | ||||||||
| Non-terminal residue | 30 | 1 | ||||||
Sequences
References
| [1] | "Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi." Feng L., Gao R., Gopalakrishnakone P. Comp. Biochem. Physiol. 148C:250-257(2008) [PubMed: 18611448] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Venom. |
Cross-references
Entry information
| Entry name | HYAL1_MESMA | ||||||||
| Accession | Primary (citable) accession number: P86100 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |