SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P86100

- HYAL1_MESMA

UniProt

P86100 - HYAL1_MESMA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Hyaluronidase-1
Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. In venom, it may participate in the degradation of the extracellular matrix thus allowing a rapid spread of other venom components during the envenomation process.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.1 Publication

Enzyme regulationi

Inhibited by heparin, and to a lesser extent by DTT, Fe3+ and Cu2+. Not inhibited by reduced glutathione, L-cysteine, EDTA, Ca2+ or Mg2+.1 Publication

pH dependencei

Optimum pH is 4.5.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius. Activity decreases when incubated at temperatures above 24 degrees Celsius for 10 minutes. No activity remains after 10 minutes at 65 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei130 – 1301Proton donor By similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB

GO - Biological processi

  1. pathogenesis Source: UniProtKB
  2. polysaccharide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-1 (EC:3.2.1.35)
Short name:
BmHYA1
Short name:
HYA1
Alternative name(s):
Hyaluronoglucosaminidase-1
Venom spreading factor
OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifieri34649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 Publication
Add
BLAST
Chaini26 – 410385Hyaluronidase-1
PRO_0000355081Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 331 By similarity
Glycosylationi48 – 481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi111 – 1111N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi199 ↔ 242 By similarity
Disulfide bondi206 ↔ 220 By similarity
Glycosylationi256 – 2561N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi356 ↔ 367 By similarity
Disulfide bondi361 ↔ 395 By similarity
Glycosylationi392 – 3921N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi397 ↔ 406 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini352 – 40756EGF-like
Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.1 Publication
Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P86100-1 [UniParc]FASTAAdd to Basket

« Hide

MTQNIQMTEM YQIILFASIL AAISATSADF KVVWEVPSIM CSKKFKINVT    50
DLLTSHKILV NQEETFNGDK IVIFYESQLG KYPHIESHGD INGGMLQVSD 100
LANHLKIARD NISKFIPDPN FNGVGIIDWE AWRPLWKYNW GRMSEYRDRS 150
KDLVKAKHPD WSPAQIEKVA IEEWENSAKE WMLKTLKLVE DMRPNAAWCY 200
YLFPDCYNYG GKDQPSEYFC KNDIQEANDK LSWLWKQSTA LCPSIYMQES 250
HITKYNTSQR AWWIYARLRE TIRLSHPNTL IYPYINYILP GTKKTVPSMD 300
FKRVLGQIGS LGLDGAIIWG SSYHVNTEEM CKEMKTYVKD VIAPVASTVI 350
QNVNRCSQQI CKGRGNCVWP EEPYTSWKYL IDPKNPTFKH TNISCKCKGG 400
YTGRYCQIAP 410
Length:410
Mass (Da):47,433
Last modified:March 19, 2014 - v2
Checksum:i4472C474E9E4DF56
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481N → C AA sequence 1 Publication
Sequence conflicti102 – 1021A → T no nucleotide entry 1 Publication
Sequence conflicti132 – 1321W → G no nucleotide entry 1 Publication
Sequence conflicti135 – 1351L → F no nucleotide entry 1 Publication
Sequence conflicti189 – 1891V → A in ACY69673. 1 Publication
Sequence conflicti235 – 2351W → G no nucleotide entry 1 Publication
Sequence conflicti329 – 3291E → G in ACY69673. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
KF588514 mRNA. Translation: AHA36327.1.
GU130249 mRNA. Translation: ACY69673.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
KF588514 mRNA. Translation: AHA36327.1 .
GU130249 mRNA. Translation: ACY69673.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR013032. EGF-like_CS.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
PRINTSi PR00846. GLHYDRLASE56.
SMARTi SM00181. EGF. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and molecular characterization of scorpion Buthus martensi venom hyaluronidases: a novel full-length and diversiform no-code stop sequences."
    Xue S., Zhao Y., Ma Y., Gui G., Huang C.
    Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and molecular characterization of BmHYA1, a novel hyaluronidase from the venom of Chinese red scorpion Buthus martensi Karsch."
    Feng L., Gao R., Meng J., Gopalakrishnakone P.
    Toxicon 56:474-479(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 29-410.
  3. "Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi."
    Feng L., Gao R., Gopalakrishnakone P.
    Comp. Biochem. Physiol. 148C:250-257(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-55, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Venom.

Entry informationi

Entry nameiHYAL1_MESMA
AccessioniPrimary (citable) accession number: P86100
Secondary accession number(s): D1MBU1, U6BKM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: March 19, 2014
Last modified: May 14, 2014
This is version 7 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi