P86100 (HYAL1_MESMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Hyaluronidase-1 Short name=HYA1 EC=3.2.1.35 Alternative name(s): Hyaluronoglucosaminidase-1
Organism	Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifier	34649 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus

Protein attributes

Sequence length	30 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides. Ref.1
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate. Ref.1
Enzyme regulation	Inhibited by heparin, and to a lesser extent by DTT, Fe3+ and Cu2+. Not inhibited by reduced glutathione, L-cysteine, EDTA, Ca2+ or Mg2+. Ref.1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Ref.1
Biophysicochemical properties	pH dependence: Optimum pH is 4.5. Ref.1 Temperature dependence: Optimum temperature is 50 degrees Celsius. Activity decreases when incubated at temperatures above 24 degrees Celsius for 10 minutes. No activity remains after 10 minutes at 65 degrees Celsius. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from direct assay Ref.1. Source: UniProtKB polysaccharide catabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	extracellular region Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	hyalurononglucosaminidase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›30	›30	Hyaluronidase-1		PRO_0000355081
Amino acid modifications
	Disulfide bond	16 ↔ ?		By similarity UniProtKB P85841
Experimental info
	Non-terminal residue	30	1

Sequences

	Sequence		Length	Mass (Da)
	P86100 [UniParc]. Last modified November 25, 2008. Version 1. Checksum: 549114125341AD85	FASTA	30	3,377
	10 20 30 TSADFKVVWE VPSIMCSKKF KICVTDLLTS

References

[1]

"Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi." Feng L., Gao R., Gopalakrishnakone P. Comp. Biochem. Physiol. 148C:250-257(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

HYAL1_MESMA

Accession

Primary (citable) accession number: P86100

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 25, 2008
Last modified:	October 3, 2012
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)