Reviewed, UniProtKB/Swiss-Prot P86085 (PME_CAPCH)

Names and origin

Protein names	Recommended name:     Pectinesterase       Short name=PE     EC=3.1.1.11 Alternative name(s):     Pectin methylesterase
Organism	Capsicum chinense (Scotch bonnet) (Bonnet pepper)
Taxonomic identifier	80379 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	22 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Pectin + n H2O = n methanol + pectate. UniProtKB P83218
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5. UniProtKB P83218
Subcellular location	Secreted › cell wall By similarity. UniProtKB P83218
Sequence similarities	Belongs to the pectinesterase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Molecular function	Aspartyl esterase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›22	›22	Pectinesterase		PRO_0000362164
Sites
	Active site	6	1	Proton donor By similarity UniProtKB P83218
	Binding site	19	1	Substrate By similarity UniProtKB P83218
	Binding site	21	1	Substrate By similarity UniProtKB P83218
	Site	5	1	Transition state stabilizer By similarity UniProtKB P83218
Experimental info
	Sequence uncertainty	1	1	I or L Ref.1
	Sequence uncertainty	4	1	F or M Ref.1
	Sequence uncertainty	5	1	Q or K Ref.1
	Sequence uncertainty	8	1	L or I Ref.1
	Sequence uncertainty	13	1	L or I Ref.1
	Sequence uncertainty	17	1	L or I Ref.1
	Sequence uncertainty	22	1	K or Q Ref.1
	Non-adjacent residues	14 – 15	2
	Non-terminal residue	1	1
	Non-terminal residue	22	1

Sequences

	Sequence		Length	Mass (Da)
	P86085-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: E7FC592B7685048D	FASTA	22	2,696
	10 20 IDAFQDTLYT HTLRTYLGRP WK

References

[1]	Almagro L., Sabater Jara A.B., Pedreno M.A. Submitted (JUL-2008) to UniProtKB Cited for: PROTEIN SEQUENCE.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR018040. Pectinesterase_AS. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. Partial match. PS00503. PECTINESTERASE_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME_CAPCH

Accession

Primary (citable) accession number: P86085

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)