Reviewed, UniProtKB/Swiss-Prot P86043 (PA21B_STRCA)

Names and origin

Protein names

Recommended name:
    Phospholipase A2
    EC=3.1.1.4
Alternative name(s):
    Phosphatidylcholine 2-acylhydrolase
    Group IB phospholipase A2

Gene names

Name:

PLA2G1B

Organism

Struthio camelus (Ostrich)

Taxonomic identifier

8801 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Palaeognathae › Struthioniformes › Struthionidae › Struthio

Protein attributes

Sequence length	22 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Ref.1
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate. Ref.1
Cofactor	Binds 1 calcium ion per subunit. Ref.1 UniProtKB Q9Z0Y2
Subcellular location	Secreted.
Sequence similarities	Belongs to the phospholipase A2 family.
Biophysicochemical properties	pH dependence: Optimum pH is 8.2. Stable when incubated for 10 minutes between pH 2 and 11. Retains 70% of its activity when incubated for 10 minutes at pH 1.5. Ref.1 Temperature dependence: Stable after incubation at 70 degrees Celsius for 10 minutes. Retains 80% of its activity after incubation at 80 degrees Celsius for 20 minutes. Ref.1

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	calcium ion binding Ref.1 Inferred from direct assay. Source: UniProtKB phospholipase A2 activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›22	›22	Phospholipase A2		PRO_0000352758
Experimental info
	Non-terminal residue	22	1

Sequences

	Sequence		Length	Mass (Da)
	P86043-1 [UniParc]. Last modified October 14, 2008. Version 1. Checksum: 5E9F478F89C0E0BB	FASTA	22	2,625
	10 20 AVWQFREMIK CTIPPSDDLL DF

References

[1]

"Ostrich pancreatic phospholipase A(2): purification and biochemical characterization." Ben Bacha A., Gargouri Y., Bezzine S., Mosbah H., Mejdoub H. J. Chromatogr. B 857:108-114(2007) [PubMed: 17656163] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES. Tissue: Pancreas.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
PROSITE	PS00119. PA2_ASP. Partial match. PS00118. PA2_HIS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_STRCA

Accession

Primary (citable) accession number: P86043

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	October 14, 2008
Last modified:	June 16, 2009
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)