Reviewed, UniProtKB/Swiss-Prot P85981 (TI_VERHE)

Names and origin

Protein names	Recommended name:     Trypsin inhibitor       Short name=VhTI
Organism	Veronica hederifolia (Ivy-leaved speedwell)
Taxonomic identifier	202477 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Plantaginaceae › Veroniceae › Veronica

Protein attributes

Sequence length	34 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits trypsin. Ref.1
Subcellular location	Secreted.
Miscellaneous	Differs dramatically in structure from all previously described families of trypsin inhibitors. Consists of a helix-loop-helix motif with two alpha-helices tightly associated by two disulfide bonds. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 34

34

Trypsin inhibitor

PRO_0000351204

Sites

Site

15 – 16

2

Reactive bond for trypsin Ref.1

Amino acid modifications

Disulfide bond

7 ↔ 29

Ref.1

Disulfide bond

11 ↔ 25

Ref.1

Secondary structure

1

.

.

.

.

.

34

Helix Strand Turn

Details...

Helix

7 – 13

7

Helix

19 – 29

11

Sequences

	Sequence		Length	Mass (Da)
	P85981-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: BD6BB312733CCE21	FASTA	34	4,050
	10 20 30 NTDPEQCKVM CYAQRHSSPE LLRRCLDNCE KEHD

References

[1]

"An unusual helix-turn-helix protease inhibitory motif in a novel trypsin inhibitor from seeds of Veronica (Veronica hederifolia L.)." Conners R., Konarev A.V., Forsyth J., Lovegrove A., Marsh J., Joseph-Horne T., Shewry P., Brady R.L. J. Biol. Chem. 282:27760-27768(2007) [PubMed: 17640870] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, REACTIVE BOND, DISULFIDE BONDS, X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) IN COMPLEX WITH BOVINE TRYPSIN. Tissue: Seed.

Cross-references

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2CMY	X-ray	2.25	B	1-34	[»]
2PLX	X-ray	1.56	B	6-31	[»]

ModBase

Search...

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

TI_VERHE

Accession

Primary (citable) accession number: P85981

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	October 13, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)