Reviewed, UniProtKB/Swiss-Prot P85974 (AGA34_AGAAL)

Names and origin

Protein names	Recommended name:     Beta-agarase AgaA34     EC=3.2.1.81
Organism	Agarivorans albus
Taxonomic identifier	182262 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Agarivorans

Protein attributes

Sequence length	10 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. Ref.1
Subcellular location	Secreted. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. Ref.1
Biophysicochemical properties	Kinetic parameters: KM=0.117 µM for agarose Ref.1 KM=0.333 µM for neoagarotetraose Ref.1 Vmax=529 µmol/min/mg enzyme toward agarose Ref.1 Vmax=313 µmol/min/mg enzyme toward neoagarotetraose Ref.1 pH dependence: Optimum pH is 8.0. Active from pH 6.0 to 10.0. Ref.1 Temperature dependence: Optimum temperature is 40 degrees Celsius. Active at temperatures up to 50 degrees Celsius. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate catabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	extracellular region Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	beta-agarase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›10	›10	Beta-agarase AgaA34		PRO_0000347325
Experimental info
	Non-terminal residue	10	1

Sequences

	Sequence		Length	Mass (Da)
	P85974-1 [UniParc]. Last modified September 2, 2008. Version 1. Checksum: 47541E19D5A1B2C7	FASTA	10	1,053
	10 ASLVTSFEEA

References

[1]

"Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34." Fu X.T., Lin H., Kim S.M. Appl. Microbiol. Biotechnol. 78:265-273(2008) [PubMed: 18071641] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION. Strain: YKW-34.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGA34_AGAAL

Accession

Primary (citable) accession number: P85974

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	March 3, 2009
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)