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P85974 (AGA34_AGAAL) Reviewed, UniProtKB/Swiss-Prot

Last modified March 6, 2013. Version 6. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-agarase AgaA34
EC=3.2.1.81
OrganismAgarivorans albus
Taxonomic identifier182262 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Agarivorans

Protein attributes

Sequence length10 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. Ref.1

Subcellular location

Secreted Ref.1.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. Ref.1

Biophysicochemical properties

Kinetic parameters:

KM=0.117 µM for agarose Ref.1

KM=0.333 µM for neoagarotetraose Ref.1

Vmax=529 µmol/min/mg enzyme toward agarose Ref.1

Vmax=313 µmol/min/mg enzyme toward neoagarotetraose Ref.1

pH dependence:

Optimum pH is 8.0. Active from pH 6.0 to 10.0. Ref.1

Temperature dependence:

Optimum temperature is 40 degrees Celsius. Active at temperatures up to 50 degrees Celsius. Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   Molecular functionGlycosidase
Hydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate catabolic process

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionbeta-agarase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›10›10Beta-agarase AgaA34
PRO_0000347325

Experimental info

Non-terminal residue101

Sequences

Sequence LengthMass (Da)Tools
P85974 [UniParc].

Last modified September 2, 2008. Version 1.
Checksum: 47541E19D5A1B2C7

FASTA101,053
        10 
ASLVTSFEEA

« Hide

References

[1]"Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34."
Fu X.T., Lin H., Kim S.M.
Appl. Microbiol. Biotechnol. 78:265-273(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
Strain: YKW-34.

Cross-references

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP85974.

Family and domain databases

PROSITEPS01034. GLYCOSYL_HYDROL_F16. Partial match.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGA34_AGAAL
AccessionPrimary (citable) accession number: P85974
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: March 6, 2013
This is version 6 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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