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P85974

- AGA34_AGAAL

UniProt

P85974 - AGA34_AGAAL

Protein

Beta-agarase AgaA34

Gene
N/A
Organism
Agarivorans albus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.1 Publication

    Kineticsi

    1. KM=0.117 µM for agarose1 Publication
    2. KM=0.333 µM for neoagarotetraose1 Publication

    Vmax=529 µmol/min/mg enzyme toward agarose1 Publication

    Vmax=313 µmol/min/mg enzyme toward neoagarotetraose1 Publication

    pH dependencei

    Optimum pH is 8.0. Active from pH 6.0 to 10.0.1 Publication

    Temperature dependencei

    Optimum temperature is 40 degrees Celsius. Active at temperatures up to 50 degrees Celsius.1 Publication

    GO - Molecular functioni

    1. beta-agarase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    SABIO-RKP85974.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-agarase AgaA341 Publication (EC:3.2.1.81)
    OrganismiAgarivorans albus
    Taxonomic identifieri182262 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAgarivorans

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›10›10Beta-agarase AgaA34PRO_0000347325

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.1 Publication

    Sequencei

    Sequence statusi: Fragment.

    P85974-1 [UniParc]FASTAAdd to Basket

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    ASLVTSFEEA                                               10
    Length:10
    Mass (Da):1,053
    Last modified:September 2, 2008 - v1
    Checksum:i47541E19D5A1B2C7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei10 – 1011 Publication

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P85974.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34."
      Fu X.T., Lin H., Kim S.M.
      Appl. Microbiol. Biotechnol. 78:265-273(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
      Strain: YKW-341 Publication.

    Entry informationi

    Entry nameiAGA34_AGAAL
    AccessioniPrimary (citable) accession number: P85974
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: September 2, 2008
    Last modified: October 1, 2014
    This is version 8 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3