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Protein

Beta-agarase AgaA34

Gene
N/A
Organism
Agarivorans albus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.1 Publication

Kineticsi

  1. KM=0.117 µM for agarose1 Publication
  2. KM=0.333 µM for neoagarotetraose1 Publication

Vmax=529 µmol/min/mg enzyme toward agarose1 Publication

Vmax=313 µmol/min/mg enzyme toward neoagarotetraose1 Publication

pH dependencei

Optimum pH is 8.0. Active from pH 6.0 to 10.0.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius. Active at temperatures up to 50 degrees Celsius.1 Publication

GO - Molecular functioni

  1. beta-agarase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP85974.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-agarase AgaA341 Publication (EC:3.2.1.81)
OrganismiAgarivorans albus
Taxonomic identifieri182262 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAgarivorans

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›10›10Beta-agarase AgaA34PRO_0000347325

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.1 Publication

Sequencei

Sequence statusi: Fragment.

P85974-1 [UniParc]FASTAAdd to basket

« Hide

        10
ASLVTSFEEA
Length:10
Mass (Da):1,053
Last modified:September 1, 2008 - v1
Checksum:i47541E19D5A1B2C7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei10 – 1011 Publication

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP85974.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Purification and characterization of a novel beta-agarase, AgaA34, from Agarivorans albus YKW-34."
    Fu X.T., Lin H., Kim S.M.
    Appl. Microbiol. Biotechnol. 78:265-273(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: YKW-341 Publication.

Entry informationi

Entry nameiAGA34_AGAAL
AccessioniPrimary (citable) accession number: P85974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2008
Last sequence update: September 1, 2008
Last modified: September 30, 2014
This is version 8 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.