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Protein

Purine nucleoside phosphorylase

Gene

Pnp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.By similarity

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.By similarity

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33PhosphateBy similarity1
Binding sitei64PhosphateBy similarity1
Binding sitei116Phosphate; via amide nitrogenBy similarity1
Binding sitei201Purine nucleosideBy similarity1
Binding sitei220PhosphateBy similarity1
Binding sitei243Purine nucleosideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-RNO-6798695. Neutrophil degranulation.
R-RNO-74217. Purine salvage.
R-RNO-74259. Purine catabolism.
UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylaseBy similarity (EC:2.4.2.1)
Short name:
PNPBy similarity
Alternative name(s):
Inosine phosphorylaseBy similarity
Inosine-guanosine phosphorylase
Gene namesi
Name:Pnp
Synonyms:Np
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 15

Organism-specific databases

RGDi1597189. Pnp.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • extracellular region Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2395.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003491261 – 289Purine nucleoside phosphorylaseAdd BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP85973.
PRIDEiP85973.

2D gel databases

World-2DPAGE0004:P85973.

PTM databases

iPTMnetiP85973.
PhosphoSitePlusiP85973.

Expressioni

Gene expression databases

ExpressionAtlasiP85973. baseline and differential.
GenevisibleiP85973. RN.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013582.

Chemistry databases

BindingDBiP85973.

Structurei

3D structure databases

ProteinModelPortaliP85973.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 86Phosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3984. Eukaryota.
COG0005. LUCA.
GeneTreeiENSGT00550000074740.
HOGENOMiHOG000045183.
HOVERGENiHBG002460.
InParanoidiP85973.
OMAiMENGYTY.
OrthoDBiEOG091G0GEE.
PhylomeDBiP85973.
TreeFamiTF300049.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000477. PurNPase. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P85973-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENEFTYEDY QRTAEWLRSH TKHRPQVAVI CGSGLGGLTA KLTQPQAFDY
60 70 80 90 100
NEIPNFPQST VQGHAGRLVF GFLNGRSCVM MQGRFHMYEG YSLSKVTFPV
110 120 130 140 150
RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FCGQNPLRGP
160 170 180 190 200
NDERFGVRFP AMSDAYDRDM RQKAFNAWKQ MGEQRELQEG TYIMSAGPTF
210 220 230 240 250
ETVAESCLLR MLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVVMDYN
260 270 280
NLEKASHQEV LEAGKAAAQK LEQFVSILME SIPPRERAN
Length:289
Mass (Da):32,302
Last modified:September 2, 2008 - v1
Checksum:i004486E55848749B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474040 Genomic DNA. Translation: EDL88431.1.
RefSeqiXP_006251919.1. XM_006251857.3.
UniGeneiRn.2738.

Genome annotation databases

EnsembliENSRNOT00000013582; ENSRNOP00000013582; ENSRNOG00000009982.
GeneIDi290029.
UCSCiRGD:1597189. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474040 Genomic DNA. Translation: EDL88431.1.
RefSeqiXP_006251919.1. XM_006251857.3.
UniGeneiRn.2738.

3D structure databases

ProteinModelPortaliP85973.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013582.

Chemistry databases

BindingDBiP85973.
ChEMBLiCHEMBL2395.

PTM databases

iPTMnetiP85973.
PhosphoSitePlusiP85973.

2D gel databases

World-2DPAGE0004:P85973.

Proteomic databases

PaxDbiP85973.
PRIDEiP85973.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013582; ENSRNOP00000013582; ENSRNOG00000009982.
GeneIDi290029.
UCSCiRGD:1597189. rat.

Organism-specific databases

CTDi4860.
RGDi1597189. Pnp.

Phylogenomic databases

eggNOGiKOG3984. Eukaryota.
COG0005. LUCA.
GeneTreeiENSGT00550000074740.
HOGENOMiHOG000045183.
HOVERGENiHBG002460.
InParanoidiP85973.
OMAiMENGYTY.
OrthoDBiEOG091G0GEE.
PhylomeDBiP85973.
TreeFamiTF300049.

Enzyme and pathway databases

UniPathwayiUPA00606.
ReactomeiR-RNO-6798695. Neutrophil degranulation.
R-RNO-74217. Purine salvage.
R-RNO-74259. Purine catabolism.

Miscellaneous databases

PROiP85973.

Gene expression databases

ExpressionAtlasiP85973. baseline and differential.
GenevisibleiP85973. RN.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000477. PurNPase. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPNPH_RAT
AccessioniPrimary (citable) accession number: P85973
Secondary accession number(s): A6KEC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 2, 2008
Last modified: November 30, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.