P85857 (GDF6A_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Growth/differentiation factor 6-A
Short name=GDF-6-A

Alternative name(s):
Growth differentiation factor 6A
Protein radar

Gene names

Name:	gdf6a
Synonyms:	radar, rdr

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functions maternally in dorsal/ventral patterning to induce the expression of the zygotic bmp2b and bmp4 genes and ventralize embryos. Zygotic expression does not appear to regulate axis specification, but instead functions to establish the integrity of the axial vessels during embryonic development. May be involved in maintaining the identity of cells of the dorsal-most neural tube and of at least a subset of neural crest cells. Ref.1 Ref.2 Ref.3 Ref.4 Ref.5
Subunit structure	Homodimer; disulfide-linked By similarity. UniProtKB P39905
Subcellular location	Secreted Probable.
Tissue specificity	First expressed in late gastrula stage embryos (9.5 hours post fertilization (hpf)) in anterior neuroectoderm corresponding to the future dorsal part of the brain. Shortly after tailbud formation (11 hpf), expression expands to the entire neural region and is subsequently expressed in derivatives of the lateral neural plate and migrating neural crest cells, with the future midbrain and hindbrain showing strong expression. Also expressed weakly and transiently in the posterior embryo from 11.5 hpf to 15 hpf in the lateral mesoderm, and in ectoderm above the neural keel. At 14 hpf, expressed along the entire length of the embryo and starting around the 16-somite stage, expressed in the dorsal quadrant of the retina, representing the distal tip of the eye anlage. At this stage, also expressed in the hatching gland and the hypochord. At 24 hpf, expressed in the roof plate outlining the fourth brain ventricle, in the posterior hypochord, the primitive gut endoderm, the ventral tail mesenchyme, the dorsal part of the neural tube and the dorsal fin. Weakly expressed in the dorsal part of the posterior spinal cord and in blood cell precursors. Ref.1 Ref.2 Ref.3 Ref.4
Developmental stage	Expressed both maternally and zygotically. Expressed from early cleavage stage until blastula sphere stage. Expression is then barely detectable until the end of gastrulation (tailbud stage) when expression is resumed. Ref.3 Ref.5
Sequence similarities	Belongs to the TGF-beta family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Developmental protein Growth factor
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from electronic annotation. Source: InterPro multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	growth factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 279	255	Potential		PRO_0000342703
	Chain	285 – 404	120	Growth/differentiation factor 6-A		PRO_0000342704
Amino acid modifications
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Disulfide bond	303 ↔ 369		By similarity UniProtKB P39905
	Disulfide bond	332 ↔ 401		By similarity UniProtKB P39905
	Disulfide bond	336 ↔ 403		By similarity UniProtKB P39905
	Disulfide bond	368		Interchain By similarity UniProtKB P39905
Experimental info
	Sequence conflict	51	1	E → R Ref.2
	Sequence conflict	70	1	V → L Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P85857 [UniParc]. Last modified July 1, 2008. Version 1. Checksum: 23233847DF96E197	FASTA	404	46,290
	10 20 30 40 50 60 MDALRAVAFY ALFVFLWSLP CCQSAALISQ KRSKGARSAF DGQRSHKFLK EILASSPGAS 70 80 90 100 110 120 RRDDFKDPVV PHDYMISIYR TYSAAEKLGL NASFFRSSKS ANTITSFVDK GKDDLTLSPL 130 140 150 160 170 180 RRQTYLFDVS TLSDKEELVG AELRIFRKSP GDVQPSPSGV YNLHLLSCRS ERPLASRSID 190 200 210 220 230 240 LQDSRKAEWE VLDVWGIFKH RHQENQLCLQ LKVTYGKSDT EIDLKQLGFH RHSRTQQERA 250 260 270 280 290 300 ILVVYTRSKK RENLFNEMKE KIKSRGDDDE EESALQFKAR RRRRTALNNR HGKRHGKKSK 310 320 330 340 350 360 SRCSKKALHV NFKELGWDDW IIAPLDYEAY HCEGVCDFPL RSHLEPTNHA IIQTLMNSMD 370 380 390 400 PNSTPPSCCV PTKLSPISIL YIDSGNNVVY KQYEDMVVEQ CGCR

References

[1]	"The BMP-related protein radar: a maintenance factor for dorsal neuroectoderm cells?" Delot E., Kataoka H., Goutel C., Yan Y.-L., Postlethwait J., Wittbrodt J., Rosa F.M. Mech. Dev. 85:15-25(1999) [PubMed: 10415343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-56, FUNCTION, TISSUE SPECIFICITY.
[2]	"Zebrafish Radar: a new member of the TGF-beta superfamily defines dorsal regions of the neural plate and the embryonic retina." Rissi M., Wittbrodt J., Delot E., Naegeli M., Rosa F.M. Mech. Dev. 49:223-234(1995) [PubMed: 7734395] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-404, FUNCTION, TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"The ventralizing activity of Radar, a maternally expressed bone morphogenetic protein, reveals complex bone morphogenetic protein interactions controlling dorso-ventral patterning in zebrafish." Goutel C., Kishimoto Y., Schulte-Merker S., Rosa F.M. Mech. Dev. 99:15-27(2000) [PubMed: 11091070] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[4]	"Radar is required for the establishment of vascular integrity in the zebrafish." Hall C.J., Flores M.V.C., Davidson A.J., Crosier K.E., Crosier P.S. Dev. Biol. 251:105-117(2002) [PubMed: 12413901] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[5]	"Maternal induction of ventral fate by zebrafish radar." Sidi S., Goutel C., Peyrieras N., Rosa F.M. Proc. Natl. Acad. Sci. U.S.A. 100:3315-3320(2003) [PubMed: 12601179] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]	"Radar breaks the fog: insights into dorsoventral patterning in zebrafish." Wilm T.P., Solnica-Krezel L. Proc. Natl. Acad. Sci. U.S.A. 100:4363-4365(2003) [PubMed: 12682283] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
IPI	IPI00488607.
RefSeq	NP_001153466.1. NM_001159994.1.
UniGene	Dr.16432.
3D structure databases
ProteinModelPortal	P85857.
SMR	P85857. Positions 300-404.
ModBase	Search...
Protein-protein interaction databases
STRING	P85857.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000075517; ENSDARP00000069999; ENSDARG00000053479.
GeneID	566470.
KEGG	dre:566470.
Organism-specific databases
CTD	566470.
Phylogenomic databases
eggNOG	fiNOG11398.
GeneTree	ENSGT00600000084167.
HOGENOM	HBG717159.
HOVERGEN	HBG107938.
OMA	RRQKYLF.
OrthoDB	EOG4S4PG9.
Gene expression databases
Bgee	P85857.
Family and domain databases
InterPro	IPR001839. TGF-b_C. IPR001111. TGF-b_N. IPR015615. TGF-beta-rel. IPR017948. TGFb_CS. [Graphical view]
KO	K04664.
PANTHER	PTHR11848. TGFbeta. 1 hit.
Pfam	PF00019. TGF_beta. 1 hit. PF00688. TGFb_propeptide. 1 hit. [Graphical view]
SMART	SM00204. TGFB. 1 hit. [Graphical view]
PROSITE	PS00250. TGF_BETA_1. 1 hit. PS51362. TGF_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GDF6A_DANRE

Accession

Primary (citable) accession number: P85857

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	July 1, 2008
Last modified:	November 16, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program