Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P85857

- GDF6A_DANRE

UniProt

P85857 - GDF6A_DANRE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Growth/differentiation factor 6-A

Gene

gdf6a

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Growth factor that controls proliferation and cellular differentiation in the retina. Plays a key role in regulating apoptosis during retinal development. Establishes dorsal-ventral positional information in the retina and controls the formation of the retinotectal map. Functions maternally in dorsal/ventral patterning to induce the expression of the zygotic bmp2b and bmp4 genes and ventralize embryos. Zygotic expression does not appear to regulate axis specification, but instead functions to establish the integrity of the axial vessels during embryonic development. May be involved in maintaining the identity of cells of the dorsal-most neural tube and of at least a subset of neural crest cells.8 Publications

GO - Biological processi

  1. apoptotic process Source: UniProtKB
  2. growth Source: InterPro
  3. multicellular organismal development Source: UniProtKB-KW
  4. retinal cell apoptotic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Growth/differentiation factor 6-A
Short name:
GDF-6-A
Alternative name(s):
Growth differentiation factor 6A
Protein radar
Gene namesi
Name:gdf6a
Synonyms:radar1 Publication, rdr1 Publication
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Chromosome 16

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Adults display microphthalmia, with eyes obscured by overgrown skin and misshapen irides. Mutants at 2 weeks of age show profound alterations in the morphology of individual photoreceptor subtypes and UV cones. At later timepoints loss of normal retinal lamination is observed, together with a disorganization of Mueller glia cells. Adults, cone photoreceptors are dysmorphic and reduced in abundance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 284260Sequence AnalysisPRO_0000342703Add
BLAST
Chaini285 – 404120Growth/differentiation factor 6-ASequence AnalysisPRO_0000342704Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi303 ↔ 369By similarity
Disulfide bondi332 ↔ 401By similarity
Disulfide bondi336 ↔ 403By similarity
Disulfide bondi368 – 368InterchainBy similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

First expressed in late gastrula stage embryos (9.5 hours post fertilization (hpf)) in anterior neuroectoderm corresponding to the future dorsal part of the brain. Shortly after tailbud formation (11 hpf), expression expands to the entire neural region and is subsequently expressed in derivatives of the lateral neural plate and migrating neural crest cells, with the future midbrain and hindbrain showing strong expression. Also expressed weakly and transiently in the posterior embryo from 11.5 hpf to 15 hpf in the lateral mesoderm, and in ectoderm above the neural keel. At 14 hpf, expressed along the entire length of the embryo and starting around the 16-somite stage, expressed in the dorsal quadrant of the retina, representing the distal tip of the eye anlage. At this stage, also expressed in the hatching gland and the hypochord. At 24 hpf, expressed in the roof plate outlining the fourth brain ventricle, in the posterior hypochord, the primitive gut endoderm, the ventral tail mesenchyme, the dorsal part of the neural tube and the dorsal fin. Weakly expressed in the dorsal part of the posterior spinal cord and in blood cell precursors.4 Publications

Developmental stagei

Expressed both maternally and zygotically. Expressed from early cleavage stage until blastula sphere stage. Expression is then barely detectable until the end of gastrulation (tailbud stage) when expression is resumed.2 Publications

Gene expression databases

BgeeiP85857.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000069999.

Structurei

3D structure databases

ProteinModelPortaliP85857.
SMRiP85857. Positions 300-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TGF-beta family.Sequence Analysis

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG317866.
GeneTreeiENSGT00760000118883.
HOGENOMiHOG000231514.
HOVERGENiHBG107938.
InParanoidiP85857.
KOiK04664.
OMAiFTRSQRK.
OrthoDBiEOG7WMCK0.
PhylomeDBiP85857.
TreeFamiTF316134.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view]
PANTHERiPTHR11848. PTHR11848. 1 hit.
PfamiPF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view]
SMARTiSM00204. TGFB. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P85857-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDALRAVAFY ALFVFLWSLP CCQSAALISQ KRSKGARSAF DGQRSHKFLK
60 70 80 90 100
EILASSPGAS RRDDFKDPVV PHDYMISIYR TYSAAEKLGL NASFFRSSKS
110 120 130 140 150
ANTITSFVDK GKDDLTLSPL RRQTYLFDVS TLSDKEELVG AELRIFRKSP
160 170 180 190 200
GDVQPSPSGV YNLHLLSCRS ERPLASRSID LQDSRKAEWE VLDVWGIFKH
210 220 230 240 250
RHQENQLCLQ LKVTYGKSDT EIDLKQLGFH RHSRTQQERA ILVVYTRSKK
260 270 280 290 300
RENLFNEMKE KIKSRGDDDE EESALQFKAR RRRRTALNNR HGKRHGKKSK
310 320 330 340 350
SRCSKKALHV NFKELGWDDW IIAPLDYEAY HCEGVCDFPL RSHLEPTNHA
360 370 380 390 400
IIQTLMNSMD PNSTPPSCCV PTKLSPISIL YIDSGNNVVY KQYEDMVVEQ

CGCR
Length:404
Mass (Da):46,290
Last modified:July 1, 2008 - v1
Checksum:i23233847DF96E197
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511E → R no nucleotide entry (PubMed:7734395)Curated
Sequence conflicti70 – 701V → L no nucleotide entry (PubMed:7734395)Curated

Sequence databases

RefSeqiNP_001153466.1. NM_001159994.1.
UniGeneiDr.16432.

Genome annotation databases

EnsembliENSDART00000075517; ENSDARP00000069999; ENSDARG00000053479.
GeneIDi566470.
KEGGidre:566470.

Cross-referencesi

Sequence databases

RefSeqi NP_001153466.1. NM_001159994.1.
UniGenei Dr.16432.

3D structure databases

ProteinModelPortali P85857.
SMRi P85857. Positions 300-404.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000069999.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSDART00000075517 ; ENSDARP00000069999 ; ENSDARG00000053479 .
GeneIDi 566470.
KEGGi dre:566470.

Organism-specific databases

CTDi 566470.

Phylogenomic databases

eggNOGi NOG317866.
GeneTreei ENSGT00760000118883.
HOGENOMi HOG000231514.
HOVERGENi HBG107938.
InParanoidi P85857.
KOi K04664.
OMAi FTRSQRK.
OrthoDBi EOG7WMCK0.
PhylomeDBi P85857.
TreeFami TF316134.

Miscellaneous databases

NextBioi 20888199.
PROi P85857.

Gene expression databases

Bgeei P85857.

Family and domain databases

Gene3Di 2.10.90.10. 1 hit.
InterProi IPR029034. Cystine-knot_cytokine.
IPR001839. TGF-b_C.
IPR001111. TGF-b_N.
IPR015615. TGF-beta-rel.
IPR017948. TGFb_CS.
[Graphical view ]
PANTHERi PTHR11848. PTHR11848. 1 hit.
Pfami PF00019. TGF_beta. 1 hit.
PF00688. TGFb_propeptide. 1 hit.
[Graphical view ]
SMARTi SM00204. TGFB. 1 hit.
[Graphical view ]
SUPFAMi SSF57501. SSF57501. 1 hit.
PROSITEi PS00250. TGF_BETA_1. 1 hit.
PS51362. TGF_BETA_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The BMP-related protein radar: a maintenance factor for dorsal neuroectoderm cells?"
    Delot E., Kataoka H., Goutel C., Yan Y.-L., Postlethwait J., Wittbrodt J., Rosa F.M.
    Mech. Dev. 85:15-25(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-56, FUNCTION, TISSUE SPECIFICITY.
  2. "Zebrafish Radar: a new member of the TGF-beta superfamily defines dorsal regions of the neural plate and the embryonic retina."
    Rissi M., Wittbrodt J., Delot E., Naegeli M., Rosa F.M.
    Mech. Dev. 49:223-234(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 51-404, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo1 Publication.
  3. "The ventralizing activity of Radar, a maternally expressed bone morphogenetic protein, reveals complex bone morphogenetic protein interactions controlling dorso-ventral patterning in zebrafish."
    Goutel C., Kishimoto Y., Schulte-Merker S., Rosa F.M.
    Mech. Dev. 99:15-27(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  4. "Radar is required for the establishment of vascular integrity in the zebrafish."
    Hall C.J., Flores M.V.C., Davidson A.J., Crosier K.E., Crosier P.S.
    Dev. Biol. 251:105-117(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  5. Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  6. "Radar breaks the fog: insights into dorsoventral patterning in zebrafish."
    Wilm T.P., Solnica-Krezel L.
    Proc. Natl. Acad. Sci. U.S.A. 100:4363-4365(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "An essential role for Radar (Gdf6a) in inducing dorsal fate in the zebrafish retina."
    Gosse N.J., Baier H.
    Proc. Natl. Acad. Sci. U.S.A. 106:2236-2241(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  9. "Molecular mechanisms regulating ocular apoptosis in zebrafish gdf6a mutants."
    Pant S.D., March L.D., Famulski J.K., French C.R., Lehmann O.J., Waskiewicz A.J.
    Invest. Ophthalmol. Vis. Sci. 54:5871-5879(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN APOPTOSIS.

Entry informationi

Entry nameiGDF6A_DANRE
AccessioniPrimary (citable) accession number: P85857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3