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Protein

Hyaluronidase 1

Gene
N/A
Organism
Tityus serrulatus (Brazilian scorpion)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides (By similarity). Is an important component of the venom, since anti-hyaluronidase serum effectively neutralizes the lethal effet of the venom injected into mice. It may act by increasing the diffusion of other venom proteins by degrading the extracellular matrix.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase 1 (EC:3.2.1.35)
Short name:
TsHyal-1
Alternative name(s):
Hyaluronoglucosaminidase
Venom spreading factor
OrganismiTityus serrulatus (Brazilian scorpion)
Taxonomic identifieri6887 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 1›1
Chaini2 – 385384Hyaluronidase 1PRO_0000343459Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi14 ↔ 307By similarity
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi173 ↔ 216By similarity
Disulfide bondi180 ↔ 194By similarity
Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi332 ↔ 343By similarity
Disulfide bondi337 ↔ 371By similarity
Disulfide bondi373 ↔ 381By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini328 – 38255EGF-likeBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Sequence Analysis
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P85841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ADFKVYWEVP SFLCSKRFKI NVTEVLTSHE ILVNQGESFN GDKIVIFYEN
60 70 80 90 100
QLGKYPHIDS NNVEINGGIL QVADLAKHLK VAKDNITKFV PNPNFNGVGV
110 120 130 140 150
IDWEAWRPSW EFNWGKLKVY KEKSIDLVKS KHPEWPSDRV EKVAKEEWEE
160 170 180 190 200
SAKEWMVKTL KLAQEMRPNA VWCYYLFPDC YNYFGKDQPS QFSCSSRIQK
210 220 230 240 250
ENSRLSWLWN QSTAICLSIY IQESHVTKYN MSQRTWWIDA RLREAIRVSE
260 270 280 290 300
HRPNIPIYPY INYILPGTNQ TVPAMDFKRT LGQIASLGLD GALLWGSSYH
310 320 330 340 350
VLTESQCKIT SDYVKSVIAP TVATVVLNTN RCSQIICKGR GNCVWPEEPF
360 370 380
SSWKYLVDPK MPVFKPTNIH CKCKGYLGRY CEIPK
Length:385
Mass (Da):44,618
Last modified:May 13, 2014 - v2
Checksum:iE0D2D508C5C3C35B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti2 – 21D → K AA sequence (Ref. 2) Curated
Sequence conflicti21 – 211N → C AA sequence (Ref. 2) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21D → Q.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF623285 mRNA. Translation: AHF72517.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF623285 mRNA. Translation: AHF72517.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular, immunological, and biological characterization of Tityus serrulatus venom hyaluronidase: new insights into its role in envenomation."
    Horta C.C., Magalhaes B.F., Oliveira-Mendes B.B., do Carmo A.O., Duarte C.G., Felicori L.F., Machado-de-Avila R.A., Chavez-Olortegui C., Kalapothakis E.
    PLoS Negl. Trop. Dis. 8:E2693-E2693(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], 3D-STRUCTURE MODELING.
    Tissue: Venom gland.
  2. "Hyaluronidase from venom of Brazilian scorpion Tityus serrulatus."
    Richardson M., Borges M.H., Cordeiro M.N., Pimenta A.M.C., de Lima M.E., Rates B.
    Submitted (APR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-35, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT GLN-2.
    Tissue: Venom1 Publication.

Entry informationi

Entry nameiHYAL1_TITSE
AccessioniPrimary (citable) accession number: P85841
Secondary accession number(s): W0HJY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 30, 2008
Last sequence update: May 13, 2014
Last modified: January 6, 2015
This is version 17 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.