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P85841

- HYAL1_TITSE

UniProt

P85841 - HYAL1_TITSE

Protein

Hyaluronidase 1

Gene
N/A
Organism
Tityus serrulatus (Brazilian scorpion)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 15 (01 Oct 2014)
      Sequence version 2 (14 May 2014)
      Previous versions | rss
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    Functioni

    Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides By similarity. Is an important component of the venom, since anti-hyaluronidase serum effectively neutralizes the lethal effet of the venom injected into mice. It may act by increasing the diffusion of other venom proteins by degrading the extracellular matrix.By similarity

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei104 – 1041Proton donorBy similarity

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase 1 (EC:3.2.1.35)
    Short name:
    TsHyal-1
    Alternative name(s):
    Hyaluronoglucosaminidase
    Venom spreading factor
    OrganismiTityus serrulatus (Brazilian scorpion)
    Taxonomic identifieri6887 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei‹1 – 1›1
    Chaini2 – 385384Hyaluronidase 1PRO_0000343459Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi14 ↔ 307By similarity
    Glycosylationi21 – 211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi85 – 851N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi173 ↔ 216By similarity
    Disulfide bondi180 ↔ 194By similarity
    Glycosylationi210 – 2101N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi269 – 2691N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi332 ↔ 343By similarity
    Disulfide bondi337 ↔ 371By similarity
    Disulfide bondi373 ↔ 381By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.1 Publication

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini328 – 38255EGF-likeBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.Sequence Analysis
    Contains 1 EGF-like domain.Curated

    Keywords - Domaini

    EGF-like domain, Signal

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
    PRINTSiPR00846. GLHYDRLASE56.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P85841-1 [UniParc]FASTAAdd to Basket

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    ADFKVYWEVP SFLCSKRFKI NVTEVLTSHE ILVNQGESFN GDKIVIFYEN    50
    QLGKYPHIDS NNVEINGGIL QVADLAKHLK VAKDNITKFV PNPNFNGVGV 100
    IDWEAWRPSW EFNWGKLKVY KEKSIDLVKS KHPEWPSDRV EKVAKEEWEE 150
    SAKEWMVKTL KLAQEMRPNA VWCYYLFPDC YNYFGKDQPS QFSCSSRIQK 200
    ENSRLSWLWN QSTAICLSIY IQESHVTKYN MSQRTWWIDA RLREAIRVSE 250
    HRPNIPIYPY INYILPGTNQ TVPAMDFKRT LGQIASLGLD GALLWGSSYH 300
    VLTESQCKIT SDYVKSVIAP TVATVVLNTN RCSQIICKGR GNCVWPEEPF 350
    SSWKYLVDPK MPVFKPTNIH CKCKGYLGRY CEIPK 385
    Length:385
    Mass (Da):44,618
    Last modified:May 14, 2014 - v2
    Checksum:iE0D2D508C5C3C35B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Sequence conflicti2 – 21D → K AA sequence 1 PublicationCurated
    Sequence conflicti21 – 211N → C AA sequence 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti2 – 21D → Q.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    KF623285 mRNA. Translation: AHF72517.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    KF623285 mRNA. Translation: AHF72517.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
    PRINTSi PR00846. GLHYDRLASE56.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular, immunological, and biological characterization of Tityus serrulatus venom hyaluronidase: new insights into its role in envenomation."
      Horta C.C., Magalhaes B.F., Oliveira-Mendes B.B., do Carmo A.O., Duarte C.G., Felicori L.F., Machado-de-Avila R.A., Chavez-Olortegui C., Kalapothakis E.
      PLoS Negl. Trop. Dis. 8:E2693-E2693(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], 3D-STRUCTURE MODELING.
      Tissue: Venom gland.
    2. "Hyaluronidase from venom of Brazilian scorpion Tityus serrulatus."
      Richardson M., Borges M.H., Cordeiro M.N., Pimenta A.M.C., de Lima M.E., Rates B.
      Submitted (MAY-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-35, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, VARIANT GLN-2.
      Tissue: Venom1 Publication.

    Entry informationi

    Entry nameiHYAL1_TITSE
    AccessioniPrimary (citable) accession number: P85841
    Secondary accession number(s): W0HJY6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: May 14, 2014
    Last modified: October 1, 2014
    This is version 15 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3