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Protein

Hyaluronidase 1

Gene
N/A
Organism
Tityus serrulatus (Brazilian scorpion)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce small oligosaccharides (By similarity). Is an important component of the venom, since anti-hyaluronidase serum effectively neutralizes the lethal effet of the venom injected into mice. It may act by increasing the diffusion of other venom proteins by degrading the extracellular matrix.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei104Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase 1 (EC:3.2.1.35)
Short name:
TsHyal-1
Alternative name(s):
Hyaluronoglucosaminidase
Venom spreading factor
OrganismiTityus serrulatus (Brazilian scorpion)
Taxonomic identifieri6887 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeTityus

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei‹1›1
ChainiPRO_00003434592 – 385Hyaluronidase 1Add BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi14 ↔ 307By similarity
Glycosylationi21N-linked (GlcNAc...)Sequence analysis1
Glycosylationi85N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi173 ↔ 216By similarity
Disulfide bondi180 ↔ 194By similarity
Glycosylationi210N-linked (GlcNAc...)Sequence analysis1
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1
Glycosylationi269N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi332 ↔ 343By similarity
Disulfide bondi337 ↔ 371By similarity
Disulfide bondi373 ↔ 381By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini328 – 382EGF-likeBy similarityAdd BLAST55

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Sequence analysis
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P85841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ADFKVYWEVP SFLCSKRFKI NVTEVLTSHE ILVNQGESFN GDKIVIFYEN
60 70 80 90 100
QLGKYPHIDS NNVEINGGIL QVADLAKHLK VAKDNITKFV PNPNFNGVGV
110 120 130 140 150
IDWEAWRPSW EFNWGKLKVY KEKSIDLVKS KHPEWPSDRV EKVAKEEWEE
160 170 180 190 200
SAKEWMVKTL KLAQEMRPNA VWCYYLFPDC YNYFGKDQPS QFSCSSRIQK
210 220 230 240 250
ENSRLSWLWN QSTAICLSIY IQESHVTKYN MSQRTWWIDA RLREAIRVSE
260 270 280 290 300
HRPNIPIYPY INYILPGTNQ TVPAMDFKRT LGQIASLGLD GALLWGSSYH
310 320 330 340 350
VLTESQCKIT SDYVKSVIAP TVATVVLNTN RCSQIICKGR GNCVWPEEPF
360 370 380
SSWKYLVDPK MPVFKPTNIH CKCKGYLGRY CEIPK
Length:385
Mass (Da):44,618
Last modified:May 14, 2014 - v2
Checksum:iE0D2D508C5C3C35B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11
Sequence conflicti2D → K AA sequence (Ref. 2) Curated1
Sequence conflicti21N → C AA sequence (Ref. 2) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2D → Q.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF623285 mRNA. Translation: AHF72517.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF623285 mRNA. Translation: AHF72517.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHYAL1_TITSE
AccessioniPrimary (citable) accession number: P85841
Secondary accession number(s): W0HJY6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: May 14, 2014
Last modified: September 7, 2016
This is version 18 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.