Reviewed, UniProtKB/Swiss-Prot P85528 (KAX6F_HEMLE)

Names and origin

Protein names	Recommended name:     Potassium channel toxin alpha-KTx 6.15 Alternative name(s):     Hemitoxin
Organism	Hemiscorpius lepturus (Scorpion)
Taxonomic identifier	520031 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Iurida › Scorpionoidea › Liochelidae

Protein attributes

Sequence length	35 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Blocks rat voltage-gated potassium channels Kv1.1 (KCNA1), Kv1.2 (KCNA2) and Kv1.3 (KCNA3) expressed in Xenopus oocytes with IC50 values of 13 nM, 16 nM and 2 nM respectively. Ref.1
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland. Ref.1
Toxic dose	LD50 is 15 µg/kg by intracerebroventricular injection into C57BL mice. Ref.1
Sequence similarities	Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor family. Alpha-KTx 6 subfamily.
Mass spectrometry	Molecular mass is 3899.24±0.67 Da from positions 1 - 35. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ionic channel inhibitor Neurotoxin Potassium channel inhibitor Toxin
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	extracellular region Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	potassium channel inhibitor activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 35	35	Potassium channel toxin alpha-KTx 6.15 Ref.1		PRO_0000341448
Amino acid modifications
	Disulfide bond	3 ↔ 24		Ref.1
	Disulfide bond	9 ↔ 29		Ref.1
	Disulfide bond	13 ↔ 19		Ref.1
	Disulfide bond	31 ↔ 34		Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P85528-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 7BF0C33C1232F457	FASTA	35	3,906
	10 20 30 IKCTLSKDCY SPCKKETGCP RAKCINRNCK CYGCS

References

[1]

"Hemitoxin, the first potassium channel toxin from the venom of the Iranian scorpion Hemiscorpius lepturus." Srairi-Abid N., Shahbazzadeh D., Chatti I., Mlayah-Bellalouna S., Mejdoub H., Borchani L., Benkhalifa R., Akbari A., El Ayeb M. FEBS J. 275:4641-4650(2008) [PubMed: 18699777] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, TOXIC DOSE, MASS SPECTROMETRY, DISULFIDE BONDS. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR001947. Scorpion_toxinS. [Graphical view]
Pfam	PF00451. Toxin_2. 1 hit. [Graphical view]
PRINTS	PR00286. CHARYBDTOXIN.
ProDom	PD003586. Scorpion_toxinS. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS01138. SCORP_SHORT_TOXIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAX6F_HEMLE

Accession

Primary (citable) accession number: P85528

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	March 3, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)