P85512 (NAHA1_PALCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-hexosaminidase EC=3.2.1.52 Alternative name(s): Beta-N-acetylhexosaminidase N-acetyl-beta-glucosaminidase NAHA1
Organism	Palythoa caribaeorum
Taxonomic identifier	134933 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Hexacorallia › Zoanthidea › Sphenopidae › Palythoa

Protein attributes

Sequence length	32 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Preferentially hydrolyzes pNP-GlcNAc, hydrolyzes pNP-GalNAc to a lesser extent. Ref.1
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. Ref.1
Enzyme regulation	Activity is decreased by HgCl2 and maltose. Activity is stimulated by Na2SeO4, BaCl2, MgCl2, chondroitin 6-sulfate and phenylmethylsulfonyl fluoride. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.53 mM for pNP-GlcNAc Ref.1 Vmax=88.1 µmol/h/mg enzyme with pNP-Glc-NAc as substrate Ref.1 pH dependence: Optimum pH is 5.0. Active over a broad range of pH values. Ref.1 Temperature dependence: Has maximum activity at 45 to 60 degrees Celsius. Inactive at temperatures of 70 degrees Celsius and above. Ref.1

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›32	›32	Beta-hexosaminidase		PRO_0000341515
Sites
	Active site	21	1	Proton donor By similarity UniProtKB P23472
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	32	1

Sequences

	Sequence		Length	Mass (Da)
	P85512 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 03DCDDB1A6FBC6F6	FASTA	32	3,557
	10 20 30 GKSSSRPLGD ATLGDLDFDI EVTQDYWDDL AR

References

[1]

"Identification of a novel beta-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)." Souza D.S.L., Grossi-de-Sa M.F., Silva L.P., Franco O.L., Gomes-Junior J.E., Oliveira G.R., Rocha T.L., Magalhaes C.P., Marra B.M., Grossi-de-Sa M., Romano E., de Sa C.M., Kombrink E., Jimenez A.V., Abreu L.R.D. Protein Expr. Purif. 58:61-69(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

3D structure databases
ProteinModelPortal	P85512.
SMR	P85512. Positions 1-32.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS01095. CHITINASE_18. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAHA1_PALCA

Accession

Primary (citable) accession number: P85512

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	March 6, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)