Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P85512

- NAHA1_PALCA

UniProt

P85512 - NAHA1_PALCA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-hexosaminidase

Gene
N/A
Organism
Palythoa caribaeorum
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Preferentially hydrolyzes pNP-GlcNAc, hydrolyzes pNP-GalNAc to a lesser extent.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.1 Publication

Enzyme regulationi

Activity is decreased by HgCl2 and maltose. Activity is stimulated by Na2SeO4, BaCl2, MgCl2, chondroitin 6-sulfate and phenylmethylsulfonyl fluoride.1 Publication

Kineticsi

  1. KM=0.53 mM for pNP-GlcNAc1 Publication

Vmax=88.1 µmol/h/mg enzyme with pNP-Glc-NAc as substrate1 Publication

pH dependencei

Optimum pH is 5.0. Active over a broad range of pH values.1 Publication

Temperature dependencei

Has maximum activity at 45 to 60 degrees Celsius. Inactive at temperatures of 70 degrees Celsius and above.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei21 – 211Proton donorBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. beta-N-acetylhexosaminidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-hexosaminidase (EC:3.2.1.52)
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
NAHA1
OrganismiPalythoa caribaeorum
Taxonomic identifieri134933 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaAnthozoaHexacoralliaZoanthariaSphenopidaePalythoa

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›32›32Beta-hexosaminidasePRO_0000341515Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP85512.
SMRiP85512. Positions 1-32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Sequencei

Sequence statusi: Fragment.

P85512-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30 
GKSSSRPLGD ATLGDLDFDI EVTQDYWDDL AR
Length:32
Mass (Da):3,557
Last modified:June 10, 2008 - v1
Checksum:i03DCDDB1A6FBC6F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 111 Publication
Non-terminal residuei32 – 3211 Publication

Cross-referencesi

3D structure databases

ProteinModelPortali P85512.
SMRi P85512. Positions 1-32.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Identification of a novel beta-N-acetylhexosaminidase (Pcb-NAHA1) from marine Zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)."
    Souza D.S.L., Grossi-de-Sa M.F., Silva L.P., Franco O.L., Gomes-Junior J.E., Oliveira G.R., Rocha T.L., Magalhaes C.P., Marra B.M., Grossi-de-Sa M., Romano E., de Sa C.M., Kombrink E., Jimenez A.V., Abreu L.R.D.
    Protein Expr. Purif. 58:61-69(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiNAHA1_PALCA
AccessioniPrimary (citable) accession number: P85512
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: October 1, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3