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P85511

- RANSM_POLLE

UniProt

P85511 - RANSM_POLLE

Protein

Ranasmurfin

Gene
N/A
Organism
Polypedates leucomystax (Common tree frog) (Hyla leucomystax)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Cofactori

    Binds 1 zinc ion per dimer.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi108 – 1081Zinc; shared with dimeric partner
    Metal bindingi112 – 1121Zinc; via tele nitrogen; shared with dimeric partner

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ranasmurfin
    Alternative name(s):
    RSF-1
    OrganismiPolypedates leucomystax (Common tree frog) (Hyla leucomystax)
    Taxonomic identifieri68444 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRhacophoridaeRhacophorinaePolypedates

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 113113RanasmurfinPRO_0000332964Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki2 ↔ 31Lysine tyrosylquinone (Tyr-Lys)1 Publication
    Modified residuei2 – 212',4',5'-topaquinone1 Publication
    Disulfide bondi4 ↔ 621 Publication
    Modified residuei9 – 91Aminomalonic acid (Ser); in chain B1 Publication
    Disulfide bondi17 ↔ 65In chain A1 Publication
    Cross-linki17 ↔ 65S-cysteinyl 3-(oxidosulfanyl)alanine (Cys-Cys); in chain B
    Cross-linki30 ↔ 108Lysine tyrosylquinone (Lys-Tyr)1 Publication
    Disulfide bondi37 ↔ 1011 Publication
    Modified residuei65 – 651Cysteine sulfenic acid (-SOH); in chain B2 Publications
    Modified residuei108 – 10812',4',5'-topaquinone1 Publication
    Cross-linki108 – 1085'-tyrosyl-5'-aminotyrosine (Tyr-Tyr) (interchain with Y-108)

    Keywords - PTMi

    Disulfide bond, LTQ, Oxidation, TPQ

    Expressioni

    Tissue specificityi

    Foam nest.1 Publication

    Interactioni

    Subunit structurei

    Homodimer. The two chains, designated A and B, differ in their modifications, but not, it is thought, in their sequence.3 Publications

    Structurei

    Secondary structure

    1
    113
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi17 – 248
    Helixi26 – 4015
    Helixi42 – 443
    Helixi46 – 6318
    Helixi76 – 783
    Helixi79 – 9012
    Helixi94 – 963
    Helixi97 – 10711

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2VH3X-ray1.16A/B1-113[»]
    ProteinModelPortaliP85511.
    SMRiP85511. Positions 3-112.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP85511.

    Sequencei

    Sequence statusi: Complete.

    P85511-1 [UniParc]FASTAAdd to Basket

    « Hide

    AYACSFPPSE IPGSKECLAE ALQKHQGFKK KSYALICAYL NYKEDAENYE    50
    RAAEDFDSAV KCTGCKEGVD LHEGNPELIE EGFEKFLASL KIDRKALGSL 100
    CTLFQKLYAI PHN 113
    Length:113
    Mass (Da):12,598
    Last modified:April 29, 2008 - v1
    Checksum:i2AE528C28C856EA7
    GO

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    About the blues - Issue 103 of March 2009

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2VH3 X-ray 1.16 A/B 1-113 [» ]
    ProteinModelPortali P85511.
    SMRi P85511. Positions 3-112.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P85511.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Unusual chromophores and crosslinks in ranasmurfin - a blue protein from the foam nests of a tropical frog."
      Muse O., Ching R.T.Y., Carter L.G., Johnson K.A., Liu H., Mcmahon S.A., White M.F., Bloch C. Jr., Botting C.H., Walsh M.A., Latiff A.A., Kennedy M.W., Cooper A., Naismith J.H.
      Submitted (FEB-2008) to UniProtKB
      Cited for: PROTEIN SEQUENCE, SUBUNIT, TISSUE SPECIFICITY, ZINC BINDING, AMINOMALONIC ACID FORMATION AT SER-9, DISULFIDE BONDS, CROSS-LINK FORMATION.
      Tissue: Foam nest1 Publication.
    2. Cited for: SUBUNIT, ZINC-BINDING, CRYSTALLIZATION.
    3. "Unusual chromophore and cross-links in ranasmurfin: a blue protein from the foam nests of a tropical frog."
      Oke M., Ching R.T.Y., Carter L.G., Johnson K.A., Liu H., McMahon S.A., White M.F., Bloch C. Jr., Botting C.H., Walsh M.A., Latiff A.A., Kennedy M.W., Cooper A., Naismith J.H.
      Angew. Chem. Int. Ed. 47:7853-7856(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.16 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT, DISULFIDE BONDS, OXIDATION AT CYS-65, CROSS-LINKS, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiRANSM_POLLE
    AccessioniPrimary (citable) accession number: P85511
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: April 29, 2008
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    After isolation, the blue protein turns green on exposure to air and sunlight.1 Publication

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

    External Data

    Dasty 3