Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P85511 (RANSM_POLLE)

Last modified November 3, 2009. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ranasmurfin
Alternative name(s):
    RSF-1
OrganismPolypedates leucomystax (Common tree frog) (Hyla leucomystax)
Taxonomic identifier68444 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraNeobatrachiaRanoideaRhacophoridaeRhacophorinaePolypedates

Protein attributes

Sequence length113 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Cofactor

Binds 1 zinc ion per dimer. Ref.1 Ref.2

Subunit structure

Homodimer. The two chains, designated A and B, differ in their modifications, but not, it is thought, in their sequence. Ref.1 Ref.2 Ref.3

Subcellular location

Secreted.

Tissue specificity

Foam nest. Ref.1

Miscellaneous

After isolation, the blue protein turns green on exposure to air and sunlight. Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   LigandMetal-binding
Zinc
   PTMDisulfide bond
LTQ
Oxidation
TPQ
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionzinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 113113Ranasmurfin
PRO_0000332964

Sites

Metal binding1081Zinc; shared with dimeric partner Ref.1
Metal binding1121Zinc; via tele nitrogen; shared with dimeric partner Ref.1

Amino acid modifications

Modified residue212',4',5'-topaquinone Ref.1
Modified residue91Aminomalonic acid (Ser); in chain B Ref.1
Modified residue651Cysteine sulfenic acid (-SOH); in chain B Ref.1
Modified residue10812',4',5'-topaquinone Ref.1
Disulfide bond4 ↔ 62 Ref.1 Ref.3
Disulfide bond17 ↔ 65In chain A Ref.1 Ref.3
Disulfide bond37 ↔ 101 Ref.1 Ref.3
Cross-link2 ↔ 31Lysine tyrosylquinone (Tyr-Lys) Ref.1
Cross-link17 ↔ 65S-cysteinyl 3-(oxidosulfanyl)alanine (Cys-Cys); in chain B Ref.1
Cross-link30 ↔ 108Lysine tyrosylquinone (Lys-Tyr) Ref.1
Cross-link1085'-tyrosyl-5'-aminotyrosine (Tyr-Tyr) (interchain) Ref.1

Secondary structure

................. 113
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P85511-1 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 2AE528C28C856EA7

FASTA11312,598
        10         20         30         40         50         60 
AYACSFPPSE IPGSKECLAE ALQKHQGFKK KSYALICAYL NYKEDAENYE RAAEDFDSAV 

        70         80         90        100        110 
KCTGCKEGVD LHEGNPELIE EGFEKFLASL KIDRKALGSL CTLFQKLYAI PHN 

« Hide

References

[1]"Unusual chromophores and crosslinks in ranasmurfin - a blue protein from the foam nests of a tropical frog."
Muse O., Ching R.T.Y., Carter L.G., Johnson K.A., Liu H., Mcmahon S.A., White M.F., Bloch C. Jr., Botting C.H., Walsh M.A., Latiff A.A., Kennedy M.W., Cooper A., Naismith J.H.
Submitted (FEB-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE, SUBUNIT, TISSUE SPECIFICITY, ZINC BINDING, AMINOMALONIC ACID FORMATION AT SER-9, DISULFIDE BONDS, CROSS-LINK FORMATION.
Tissue: Foam nest.
[2]"Crystallization of ranasmurfin, a blue-coloured protein from Polypedates leucomystax."
McMahon S.A., Walsh M.A., Ching R.T.Y., Carter L.G., Dorward M., Johnson K.A., Liu H., Oke M., Bloch C. Jr., Kennedy M.W., Latiff A.A., Cooper A., Taylor G.L., White M.F., Naismith J.H.
Acta Crystallogr. F 62:1124-1126(2006) [PubMed: 17077494] [Abstract]
Cited for: SUBUNIT, ZINC-BINDING, CRYSTALLIZATION.
[3]"Unusual chromophore and cross-links in ranasmurfin: a blue protein from the foam nests of a tropical frog."
Oke M., Ching R.T.Y., Carter L.G., Johnson K.A., Liu H., McMahon S.A., White M.F., Bloch C. Jr., Botting C.H., Walsh M.A., Latiff A.A., Kennedy M.W., Cooper A., Naismith J.H.
Angew. Chem. Int. Ed. 47:7853-7856(2008) [PubMed: 18781570] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.16 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT, DISULFIDE BONDS, CROSS-LINKS, MASS SPECTROMETRY.

Web resources

Protein Spotlight

About the blues - Issue 103 of March 2009

Cross-references

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2VH3X-ray1.16A/B1-113[»]
ModBaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameRANSM_POLLE
AccessionPrimary (citable) accession number: P85511
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents