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P85511 (RANSM_POLLE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length113 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Cofactor

Binds 1 zinc ion per dimer. Ref.1 Ref.2

Subunit structure

Homodimer. The two chains, designated A and B, differ in their modifications, but not, it is thought, in their sequence. Ref.1 Ref.2 Ref.3

Subcellular location

Secreted.

Tissue specificity

Foam nest. Ref.1

Miscellaneous

After isolation, the blue protein turns green on exposure to air and sunlight. Ref.1

Ontologies

Keywords
   Cellular componentSecreted
   LigandMetal-binding
Zinc
   PTMDisulfide bond
LTQ
Oxidation
TPQ
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 113113Ranasmurfin
PRO_0000332964

Sites

Metal binding1081Zinc; shared with dimeric partner Ref.1
Metal binding1121Zinc; via tele nitrogen; shared with dimeric partner Ref.1

Amino acid modifications

Modified residue212',4',5'-topaquinone Ref.1
Modified residue91Aminomalonic acid (Ser); in chain B Ref.1
Modified residue651Cysteine sulfenic acid (-SOH); in chain B Ref.1
Modified residue10812',4',5'-topaquinone Ref.1
Disulfide bond4 ↔ 62 Ref.1 Ref.3
Disulfide bond17 ↔ 65In chain A Ref.1 Ref.3
Disulfide bond37 ↔ 101 Ref.1 Ref.3
Cross-link2 ↔ 31Lysine tyrosylquinone (Tyr-Lys) Ref.1
Cross-link17 ↔ 65S-cysteinyl 3-(oxidosulfanyl)alanine (Cys-Cys); in chain B Ref.1
Cross-link30 ↔ 108Lysine tyrosylquinone (Lys-Tyr) Ref.1
Cross-link1085'-tyrosyl-5'-aminotyrosine (Tyr-Tyr) (interchain with Y-108) Ref.1

Secondary structure

............... 113
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P85511 [UniParc].

Last modified April 29, 2008. Version 1.
Checksum: 2AE528C28C856EA7

FASTA11312,598
        10         20         30         40         50         60 
AYACSFPPSE IPGSKECLAE ALQKHQGFKK KSYALICAYL NYKEDAENYE RAAEDFDSAV 

        70         80         90        100        110 
KCTGCKEGVD LHEGNPELIE EGFEKFLASL KIDRKALGSL CTLFQKLYAI PHN 

« Hide

References

[1]"Unusual chromophores and crosslinks in ranasmurfin - a blue protein from the foam nests of a tropical frog."
Muse O., Ching R.T.Y., Carter L.G., Johnson K.A., Liu H., Mcmahon S.A., White M.F., Bloch C. Jr., Botting C.H., Walsh M.A., Latiff A.A., Kennedy M.W., Cooper A., Naismith J.H.
Submitted (FEB-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE, SUBUNIT, TISSUE SPECIFICITY, ZINC BINDING, AMINOMALONIC ACID FORMATION AT SER-9, DISULFIDE BONDS, CROSS-LINK FORMATION.
Tissue: Foam nest.
[2]"Crystallization of ranasmurfin, a blue-coloured protein from Polypedates leucomystax."
McMahon S.A., Walsh M.A., Ching R.T.Y., Carter L.G., Dorward M., Johnson K.A., Liu H., Oke M., Bloch C. Jr., Kennedy M.W., Latiff A.A., Cooper A., Taylor G.L., White M.F., Naismith J.H.
Acta Crystallogr. F 62:1124-1126(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT, ZINC-BINDING, CRYSTALLIZATION.
[3]"Unusual chromophore and cross-links in ranasmurfin: a blue protein from the foam nests of a tropical frog."
Oke M., Ching R.T.Y., Carter L.G., Johnson K.A., Liu H., McMahon S.A., White M.F., Bloch C. Jr., Botting C.H., Walsh M.A., Latiff A.A., Kennedy M.W., Cooper A., Naismith J.H.
Angew. Chem. Int. Ed. 47:7853-7856(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.16 ANGSTROMS) IN COMPLEX WITH ZINC IONS, SUBUNIT, DISULFIDE BONDS, CROSS-LINKS, IDENTIFICATION BY MASS SPECTROMETRY.

Web resources

Protein Spotlight

About the blues - Issue 103 of March 2009

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2VH3X-ray1.16A/B3-113[»]
ProteinModelPortalP85511.
SMRP85511. Positions 3-112.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Other

EvolutionaryTraceP85511.

Entry information

Entry nameRANSM_POLLE
AccessionPrimary (citable) accession number: P85511
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: April 16, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references