Reviewed, UniProtKB/Swiss-Prot P85430 (LAC2D_CERUI)

Names and origin

Protein names	Recommended name:     Laccase-2d     EC=1.10.3.2 Alternative name(s):     Laccase-IId       Short name=Lac-IId     Benzenediol:oxygen oxidoreductase     Urishiol oxidase     Diphenol oxidase
Organism	Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifier	90312 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Cerrena

Protein attributes

Sequence length	47 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products Probable. Has highest activity towards ABTS, also active towards ferulic acid and guaiacol, but is not active towards tyrosine, vanillic acid, 2,5-dimethyl aniline, p-anisidine or violuric acid.
Catalytic activity	4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O. UniProtKB Q99044
Cofactor	Binds 4 copper ions per monomer By similarity. UniProtKB Q12718
Enzyme regulation	Inhibited by sodium azide, SDS and mercaptoethanol, but not by 4-hexyl resocinol, L-cysteine and dithiothreitol. Activity is inhibited by the heavy metal ions Cr, W, Sn, Ag+ and Hg2+, but not by Pb2+, Fe3+, Ni2+, Li2+, Co2+ or Cd2+.
Subunit structure	Homodimer By similarity. UniProtKB Q99044
Subcellular location	Secreted.
Post-translational modification	N-glycosylated; contains 17% carbohydrates.
Miscellaneous	On the 2D-gel the determined pI of this protein is: 5.3, its MW is: 59 kDa.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.
biophysicochemical properties	Kinetic parameters: KM=54.1 µM for ABTS (at 70 degrees Celsius) KM=57.1 µM for ABTS (at 30 degrees Celsius) KM=19.2 µM for syringaldizine (at 30 degrees Celsius) pH dependence: Optimum pH is 3.0 at 70 degrees Celsius with ABTS as substrate, and 6.0 with guaiacol and syringaldazine as substrate. Temperature dependence: Optimum temperature is 70 degrees Celsius at pH 3.0 with ABTS as substrate. Retains 100% of its activity after 1 hour at 30 degrees Celsius at pH 9.0. Retains more than 60% of its activity after 180 minutes at 60 degrees Celsius at pH 9.0. Retains approximately 50% of its activity after 90 minutes at 70 degrees Celsius at pH 9.0.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Domain	Repeat
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›47	›47	Laccase-2d		PRO_0000320022
Regions
	Domain	2 – ?		Plastocyanin-like 1
	Domain	? – ›47		Plastocyanin-like 3
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-adjacent residues	30 – 31	2
	Non-terminal residue	47	1

Sequences

	Sequence		Length	Mass (Da)
	P85430-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: DFC1942A188FC2B9	FASTA	47	4,846
	10 20 30 40 GTGPVADLHI INKDLSPDGF QRPTVVAGGG RDVVSIGRAG DNVTIRF

References

[1]

"A thermostable metal-tolerant laccase with bioremediation potential from a marine-derived fungus." D'Souza-Ticlo D., Sharma D., Raghukumar C. Mar. Biotechnol. 0:0-0(2009) [PubMed: 19283431] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION. Strain: MTCC 5159.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002355. Cu_oxidase_Cu_BS. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. Partial match. PS00080. MULTICOPPER_OXIDASE2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC2D_CERUI

Accession

Primary (citable) accession number: P85430

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 2, 2008
Last modified:	June 16, 2009
This is version 8 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)