Reviewed, UniProtKB/Swiss-Prot P85430 (LAC2D_CERSM)

Names and origin

Protein names	Recommended name:     Laccase-2d     EC=1.10.3.2 Alternative name(s):     Laccase-IId       Short name=Lac-IId     Benzenediol:oxygen oxidoreductase     Urishiol oxidase     Diphenol oxidase
Organism	Cerrena sp. (strain MTCC 5159) (Bracket fungus)
Taxonomic identifier	411686 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Cerrena

Protein attributes

Sequence length	47 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products Probable.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted.
Post-translational modification	N-glycosylated; contains 15.5% carbohydrates.
Miscellaneous	On the 2D-gel the determined pI of this protein is: 5.3, its MW is: 58.5 kDa.
Sequence similarities	Belongs to the multicopper oxidase family. Contains 3 plastocyanin-like domains.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Domain	Repeat
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›47	›47	Laccase-2d		PRO_0000320022
Regions
	Domain	2 – ?		Plastocyanin-like 1
	Domain	? – ›47		Plastocyanin-like 3
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-adjacent residues	30 – 31	2
	Non-terminal residue	47	1

Sequences

	Sequence		Length	Mass (Da)
	P85430-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: DFC1942A188FC2B9	FASTA	47	4,846
	10 20 30 40 GTGPVADLHI INKDLSPDGF QRPTVVAGGG RDVVSIGRAG DNVTIRF

References

[1]	"Sequence of laccase from Cerrena sp. MTCC 5159." D'Souza Ticlo D.T., Sharma D., Raghukumar C. Submitted (JUL-2008) to UniProtKB Cited for: PROTEIN SEQUENCE, GLYCOSYLATION.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002355. Cu_oxidase_Cu_BS. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. Partial match. PS00080. MULTICOPPER_OXIDASE2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC2D_CERSM

Accession

Primary (citable) accession number: P85430

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	September 2, 2008
Last modified:	November 4, 2008
This is version 6 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)