Reviewed, UniProtKB/Swiss-Prot P85308 (LAC1B_CERUI)

Names and origin

Protein names	Recommended name:     Laccase-1b     EC=1.10.3.2 Alternative name(s):     Benzenediol:oxygen oxidoreductase 1b     Urishiol oxidase 1b     Diphenol oxidase 1b     Lac Ib
Organism	Cerrena unicolor (Canker rot fungus) (Daedalea unicolor)
Taxonomic identifier	90312 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Aphyllophorales › Cerrena

Protein attributes

Sequence length	9 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lignin degradation and detoxification of lignin-derived products Probable.
Catalytic activity	4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O.
Cofactor	Binds 4 copper ions per monomer By similarity.
Subcellular location	SecretedBy similarity.
Miscellaneous	On the 2D-gel the determined pI of this protein is: 4.27, its MW is: 54.49 kDa.
Sequence similarities	Belongs to the multicopper oxidase family.
Biophysicochemical properties	Kinetic parameters: KM=0.153 mM for syringaldazine pH dependence: Optimum pH is 5.3. Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Biological process	Lignin degradation
Cellular component	Secreted
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lignin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW laccase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›9	›9	Laccase-1b		PRO_0000310833
Experimental info
	Non-terminal residue	9	1

Sequences

	Sequence		Length	Mass (Da)
	P85308-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 1CE22AADC2D76870	FASTA	9	892
	AIGPVADLH

References

[1]	Checinska A., Janusz G., Rogalski J.M. Submitted (OCT-2007) to UniProtKB Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR002355. Cu_oxidase_Cu_BS. [Graphical view]
PROSITE	PS00079. MULTICOPPER_OXIDASE1. Partial match. PS00080. MULTICOPPER_OXIDASE2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LAC1B_CERUI

Accession

Primary (citable) accession number: P85308

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	November 4, 2008
This is version 5 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)