Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P85302

- AMPC_PSEFL

UniProt

P85302 - AMPC_PSEFL

Protein

Beta-lactamase

Gene

ampC

Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 23 (01 Oct 2014)
      Sequence version 1 (23 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.Curated

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.1 PublicationPROSITE-ProRule annotation

    Kineticsi

    1. KM=120 µM for nitrocefin2 Publications
    2. KM=116 µM for nitrocefin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
    3. KM=50 µM for cephalexin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
    4. KM=2.3 µM for benzylpenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
    5. KM=5.1 µM for ampicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
    6. KM=3.7 µM for carbenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
    7. KM=0.118 µM for oxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
    8. KM=0.016 µM for cloxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei60 – 601Acyl-ester intermediateBy similarityPROSITE-ProRule annotation
    Active sitei146 – 1461Proton acceptorBy similarity

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3247-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamaseBy similarity2 Publications (EC:3.5.2.6)
    Alternative name(s):
    CephalosporinaseBy similarity2 Publications
    Gene namesi
    Name:ampCBy similarity
    OrganismiPseudomonas fluorescens
    Taxonomic identifieri294 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    Periplasm 1 Publication

    GO - Cellular componenti

    1. outer membrane-bounded periplasmic space Source: InterPro

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Beta-lactamasePRO_0000308487Add
    BLAST

    Structurei

    Secondary structure

    1
    358
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815
    Beta strandi22 – 309
    Beta strandi33 – 4210
    Turni44 – 463
    Beta strandi55 – 573
    Helixi59 – 613
    Helixi62 – 7514
    Helixi85 – 884
    Helixi90 – 923
    Helixi96 – 983
    Beta strandi99 – 1013
    Helixi102 – 1065
    Helixi125 – 1339
    Turni140 – 1423
    Helixi148 – 16013
    Turni161 – 1633
    Helixi166 – 1727
    Turni173 – 1753
    Helixi176 – 1783
    Beta strandi181 – 1866
    Helixi189 – 1946
    Helixi214 – 2174
    Helixi224 – 23512
    Helixi237 – 2393
    Helixi242 – 25110
    Beta strandi253 – 2586
    Beta strandi261 – 2633
    Beta strandi268 – 2736
    Helixi276 – 2827
    Helixi286 – 2894
    Beta strandi295 – 3017
    Beta strandi305 – 31410
    Beta strandi319 – 3257
    Turni326 – 3294
    Beta strandi330 – 3389
    Helixi342 – 35514

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QZ6X-ray2.26A1-358[»]
    ProteinModelPortaliP85302.
    SMRiP85302. Positions 3-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP85302.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni311 – 3133Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-C beta-lactamase family.Sequence Analysis

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P85302-1 [UniParc]FASTAAdd to Basket

    « Hide

    ATDIRQVVDS TVEPLMQQQD IAGLSVAVIQ NGKAQYFNYG VANKDSKQPI    50
    TENTLFEIGS VSKTFTATLA GYALANGKLK LSDPASQYLP ALRGDKFDHI 100
    SLLNLGTYTA GGLPLQFPEE SDNTGKMISY YQHWKPAFAP GTQRLYSNPS 150
    IGLFGHLAAQ SLGQPFEKLM EQTVLPKLGL KHTFISVPET QMSLYAQGYD 200
    KAGKPVRVSP GALDAEAYGI KTSTSDLIHY VEVNMHPAKL EKPLQQAIAA 250
    THTGYYTVDG MTQGLGWEMY PYPIKVDALV EGNSTQMAME PHKVNWLTPP 300
    QAAPLDTLVN KTGSTGGFGA YVAYVPSKGL GVVILANKNY PNAERVKAAH 350
    AILSAMDQ 358
    Length:358
    Mass (Da):38,728
    Last modified:October 23, 2007 - v1
    Checksum:iC2C35CB71DF9E501
    GO

    Mass spectrometryi

    Molecular mass is 38723.1±4.9 Da from positions 1 - 358. Determined by ESI. 1 Publication

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2QZ6 X-ray 2.26 A 1-358 [» ]
    ProteinModelPortali P85302.
    SMRi P85302. Positions 3-356.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-3247-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P85302.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view ]
    Pfami PF00144. Beta-lactamase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY.
      Strain: TAE 41 Publication.
    2. "Inducible class C beta-lactamases produced by psychrophilic bacteria."
      Pierrard A., Ledent P., Docquier J.-D., Feller G., Gerday C., Frere J.-M.
      FEMS Microbiol. Lett. 161:311-315(1998)
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiAMPC_PSEFL
    AccessioniPrimary (citable) accession number: P85302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: October 1, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3