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Protein

Beta-lactamase

Gene

ampC

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.Curated

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation1 Publication

Kineticsi

  1. KM=120 µM for nitrocefin2 Publications
  2. KM=116 µM for nitrocefin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  3. KM=50 µM for cephalexin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  4. KM=2.3 µM for benzylpenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  5. KM=5.1 µM for ampicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  6. KM=3.7 µM for carbenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  7. KM=0.118 µM for oxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  8. KM=0.016 µM for cloxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei60 – 601Acyl-ester intermediatePROSITE-ProRule annotationBy similarity
    Active sitei146 – 1461Proton acceptorBy similarity

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3247-MONOMER.
    BRENDAi3.5.2.6. 5121.

    Protein family/group databases

    MEROPSiS12.006.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase2 PublicationsBy similarity (EC:3.5.2.6)
    Alternative name(s):
    Cephalosporinase2 PublicationsBy similarity
    Gene namesi
    Name:ampCBy similarity
    OrganismiPseudomonas fluorescens
    Taxonomic identifieri294 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 358358Beta-lactamasePRO_0000308487Add
    BLAST

    Structurei

    Secondary structure

    1
    358
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815Combined sources
    Beta strandi22 – 309Combined sources
    Beta strandi33 – 4210Combined sources
    Turni44 – 463Combined sources
    Beta strandi55 – 573Combined sources
    Helixi59 – 613Combined sources
    Helixi62 – 7514Combined sources
    Helixi85 – 884Combined sources
    Helixi90 – 923Combined sources
    Helixi96 – 983Combined sources
    Beta strandi99 – 1013Combined sources
    Helixi102 – 1065Combined sources
    Helixi125 – 1339Combined sources
    Turni140 – 1423Combined sources
    Helixi148 – 16013Combined sources
    Turni161 – 1633Combined sources
    Helixi166 – 1727Combined sources
    Turni173 – 1753Combined sources
    Helixi176 – 1783Combined sources
    Beta strandi181 – 1866Combined sources
    Helixi189 – 1946Combined sources
    Helixi214 – 2174Combined sources
    Helixi224 – 23512Combined sources
    Helixi237 – 2393Combined sources
    Helixi242 – 25110Combined sources
    Beta strandi253 – 2586Combined sources
    Beta strandi261 – 2633Combined sources
    Beta strandi268 – 2736Combined sources
    Helixi276 – 2827Combined sources
    Helixi286 – 2894Combined sources
    Beta strandi295 – 3017Combined sources
    Beta strandi305 – 31410Combined sources
    Beta strandi319 – 3257Combined sources
    Turni326 – 3294Combined sources
    Beta strandi330 – 3389Combined sources
    Helixi342 – 35514Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QZ6X-ray2.26A1-358[»]
    ProteinModelPortaliP85302.
    SMRiP85302. Positions 3-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP85302.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni311 – 3133Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-C beta-lactamase family.Sequence Analysis

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P85302-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    ATDIRQVVDS TVEPLMQQQD IAGLSVAVIQ NGKAQYFNYG VANKDSKQPI
    60 70 80 90 100
    TENTLFEIGS VSKTFTATLA GYALANGKLK LSDPASQYLP ALRGDKFDHI
    110 120 130 140 150
    SLLNLGTYTA GGLPLQFPEE SDNTGKMISY YQHWKPAFAP GTQRLYSNPS
    160 170 180 190 200
    IGLFGHLAAQ SLGQPFEKLM EQTVLPKLGL KHTFISVPET QMSLYAQGYD
    210 220 230 240 250
    KAGKPVRVSP GALDAEAYGI KTSTSDLIHY VEVNMHPAKL EKPLQQAIAA
    260 270 280 290 300
    THTGYYTVDG MTQGLGWEMY PYPIKVDALV EGNSTQMAME PHKVNWLTPP
    310 320 330 340 350
    QAAPLDTLVN KTGSTGGFGA YVAYVPSKGL GVVILANKNY PNAERVKAAH

    AILSAMDQ
    Length:358
    Mass (Da):38,728
    Last modified:October 23, 2007 - v1
    Checksum:iC2C35CB71DF9E501
    GO

    Mass spectrometryi

    Molecular mass is 38723.1±4.9 Da from positions 1 - 358. Determined by ESI. 1 Publication

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2QZ6X-ray2.26A1-358[»]
    ProteinModelPortaliP85302.
    SMRiP85302. Positions 3-356.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    MEROPSiS12.006.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3247-MONOMER.
    BRENDAi3.5.2.6. 5121.

    Miscellaneous databases

    EvolutionaryTraceiP85302.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    1. Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY.
      Strain: TAE 41 Publication.
    2. "Inducible class C beta-lactamases produced by psychrophilic bacteria."
      Pierrard A., Ledent P., Docquier J.-D., Feller G., Gerday C., Frere J.-M.
      FEMS Microbiol. Lett. 161:311-315(1998)
      Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiAMPC_PSEFL
    AccessioniPrimary (citable) accession number: P85302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: April 1, 2015
    This is version 26 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.