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P85302

- AMPC_PSEFL

UniProt

P85302 - AMPC_PSEFL

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Protein
Beta-lactamase
Gene
ampC
Organism
Pseudomonas fluorescens
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.1 Publication

Kineticsi

  1. KM=120 µM for nitrocefin2 Publications
  2. KM=116 µM for nitrocefin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)1 Publication
  3. KM=50 µM for cephalexin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)
  4. KM=2.3 µM for benzylpenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)1 Publication
  5. KM=5.1 µM for ampicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)
  6. KM=3.7 µM for carbenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)
  7. KM=0.118 µM for oxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)
  8. KM=0.016 µM for cloxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei60 – 601Acyl-ester intermediate By similarityBy similarity
Active sitei146 – 1461Proton acceptor By similarityBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3247-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase (EC:3.5.2.6)
Alternative name(s):
Cephalosporinase
Gene namesi
Name:ampC
OrganismiPseudomonas fluorescens
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Subcellular locationi

Periplasm 1 Publication

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Beta-lactamase
PRO_0000308487Add
BLAST

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815
Beta strandi22 – 309
Beta strandi33 – 4210
Turni44 – 463
Beta strandi55 – 573
Helixi59 – 613
Helixi62 – 7514
Helixi85 – 884
Helixi90 – 923
Helixi96 – 983
Beta strandi99 – 1013
Helixi102 – 1065
Helixi125 – 1339
Turni140 – 1423
Helixi148 – 16013
Turni161 – 1633
Helixi166 – 1727
Turni173 – 1753
Helixi176 – 1783
Beta strandi181 – 1866
Helixi189 – 1946
Helixi214 – 2174
Helixi224 – 23512
Helixi237 – 2393
Helixi242 – 25110
Beta strandi253 – 2586
Beta strandi261 – 2633
Beta strandi268 – 2736
Helixi276 – 2827
Helixi286 – 2894
Beta strandi295 – 3017
Beta strandi305 – 31410
Beta strandi319 – 3257
Turni326 – 3294
Beta strandi330 – 3389
Helixi342 – 35514

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QZ6X-ray2.26A1-358[»]
ProteinModelPortaliP85302.
SMRiP85302. Positions 3-356.

Miscellaneous databases

EvolutionaryTraceiP85302.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni311 – 3133Substrate binding By similarityBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view]
PfamiPF00144. Beta-lactamase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P85302-1 [UniParc]FASTAAdd to Basket

« Hide

ATDIRQVVDS TVEPLMQQQD IAGLSVAVIQ NGKAQYFNYG VANKDSKQPI    50
TENTLFEIGS VSKTFTATLA GYALANGKLK LSDPASQYLP ALRGDKFDHI 100
SLLNLGTYTA GGLPLQFPEE SDNTGKMISY YQHWKPAFAP GTQRLYSNPS 150
IGLFGHLAAQ SLGQPFEKLM EQTVLPKLGL KHTFISVPET QMSLYAQGYD 200
KAGKPVRVSP GALDAEAYGI KTSTSDLIHY VEVNMHPAKL EKPLQQAIAA 250
THTGYYTVDG MTQGLGWEMY PYPIKVDALV EGNSTQMAME PHKVNWLTPP 300
QAAPLDTLVN KTGSTGGFGA YVAYVPSKGL GVVILANKNY PNAERVKAAH 350
AILSAMDQ 358
Length:358
Mass (Da):38,728
Last modified:October 23, 2007 - v1
Checksum:iC2C35CB71DF9E501
GO

Mass spectrometryi

Molecular mass is 38723.1±4.9 Da from positions 1 - 358. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2QZ6 X-ray 2.26 A 1-358 [» ]
ProteinModelPortali P85302.
SMRi P85302. Positions 3-356.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-3247-MONOMER.

Miscellaneous databases

EvolutionaryTracei P85302.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR001586. Beta-lactam_class-C_AS.
[Graphical view ]
Pfami PF00144. Beta-lactamase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00336. BETA_LACTAMASE_C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY.
    Strain: TAE 4.
  2. "Inducible class C beta-lactamases produced by psychrophilic bacteria."
    Pierrard A., Ledent P., Docquier J.-D., Feller G., Gerday C., Frere J.-M.
    FEMS Microbiol. Lett. 161:311-315(1998)
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAMPC_PSEFL
AccessioniPrimary (citable) accession number: P85302
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: July 9, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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