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Protein

Beta-lactamase

Gene

ampC

Organism
Pseudomonas fluorescens
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.Curated

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation1 Publication

Kineticsi

  1. KM=120 µM for nitrocefin2 Publications
  2. KM=116 µM for nitrocefin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  3. KM=50 µM for cephalexin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  4. KM=2.3 µM for benzylpenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  5. KM=5.1 µM for ampicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  6. KM=3.7 µM for carbenicillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  7. KM=0.118 µM for oxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications
  8. KM=0.016 µM for cloxacillin (at 30 degrees Celsius in 50 mM sodium phosphate, pH 7.0)2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei60Acyl-ester intermediatePROSITE-ProRule annotationBy similarity1
    Active sitei146Proton acceptorBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BRENDAi3.5.2.6. 5121.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase2 PublicationsBy similarity (EC:3.5.2.6)
    Alternative name(s):
    Cephalosporinase2 PublicationsBy similarity
    Gene namesi
    Name:ampCBy similarity
    OrganismiPseudomonas fluorescens
    Taxonomic identifieri294 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003084871 – 358Beta-lactamaseAdd BLAST358

    Structurei

    Secondary structure

    1358
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi4 – 18Combined sources15
    Beta strandi22 – 30Combined sources9
    Beta strandi33 – 42Combined sources10
    Turni44 – 46Combined sources3
    Beta strandi55 – 57Combined sources3
    Helixi59 – 61Combined sources3
    Helixi62 – 75Combined sources14
    Helixi85 – 88Combined sources4
    Helixi90 – 92Combined sources3
    Helixi96 – 98Combined sources3
    Beta strandi99 – 101Combined sources3
    Helixi102 – 106Combined sources5
    Helixi125 – 133Combined sources9
    Turni140 – 142Combined sources3
    Helixi148 – 160Combined sources13
    Turni161 – 163Combined sources3
    Helixi166 – 172Combined sources7
    Turni173 – 175Combined sources3
    Helixi176 – 178Combined sources3
    Beta strandi181 – 186Combined sources6
    Helixi189 – 194Combined sources6
    Helixi214 – 217Combined sources4
    Helixi224 – 235Combined sources12
    Helixi237 – 239Combined sources3
    Helixi242 – 251Combined sources10
    Beta strandi253 – 258Combined sources6
    Beta strandi261 – 263Combined sources3
    Beta strandi268 – 273Combined sources6
    Helixi276 – 282Combined sources7
    Helixi286 – 289Combined sources4
    Beta strandi295 – 301Combined sources7
    Beta strandi305 – 314Combined sources10
    Beta strandi319 – 325Combined sources7
    Turni326 – 329Combined sources4
    Beta strandi330 – 338Combined sources9
    Helixi342 – 355Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2QZ6X-ray2.26A1-358[»]
    ProteinModelPortaliP85302.
    SMRiP85302.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP85302.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni311 – 313Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the class-C beta-lactamase family.Sequence analysis

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P85302-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    ATDIRQVVDS TVEPLMQQQD IAGLSVAVIQ NGKAQYFNYG VANKDSKQPI
    60 70 80 90 100
    TENTLFEIGS VSKTFTATLA GYALANGKLK LSDPASQYLP ALRGDKFDHI
    110 120 130 140 150
    SLLNLGTYTA GGLPLQFPEE SDNTGKMISY YQHWKPAFAP GTQRLYSNPS
    160 170 180 190 200
    IGLFGHLAAQ SLGQPFEKLM EQTVLPKLGL KHTFISVPET QMSLYAQGYD
    210 220 230 240 250
    KAGKPVRVSP GALDAEAYGI KTSTSDLIHY VEVNMHPAKL EKPLQQAIAA
    260 270 280 290 300
    THTGYYTVDG MTQGLGWEMY PYPIKVDALV EGNSTQMAME PHKVNWLTPP
    310 320 330 340 350
    QAAPLDTLVN KTGSTGGFGA YVAYVPSKGL GVVILANKNY PNAERVKAAH

    AILSAMDQ
    Length:358
    Mass (Da):38,728
    Last modified:October 23, 2007 - v1
    Checksum:iC2C35CB71DF9E501
    GO

    Mass spectrometryi

    Molecular mass is 38723.1±4.9 Da from positions 1 - 358. Determined by ESI. 1 Publication

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2QZ6X-ray2.26A1-358[»]
    ProteinModelPortaliP85302.
    SMRiP85302.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Enzyme and pathway databases

    BRENDAi3.5.2.6. 5121.

    Miscellaneous databases

    EvolutionaryTraceiP85302.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR001466. Beta-lactam-related.
    IPR012338. Beta-lactam/transpept-like.
    IPR001586. Beta-lactam_class-C_AS.
    [Graphical view]
    PfamiPF00144. Beta-lactamase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00336. BETA_LACTAMASE_C. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAMPC_PSEFL
    AccessioniPrimary (citable) accession number: P85302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: October 23, 2007
    Last modified: November 2, 2016
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.