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Protein

Proline-rich protein 5

Gene

PRR5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

ReactomeiR-HSA-1257604. PIP3 activates AKT signaling.
R-HSA-389357. CD28 dependent PI3K/Akt signaling.
R-HSA-5218920. VEGFR2 mediated vascular permeability.
R-HSA-5674400. Constitutive Signaling by AKT1 E17K in Cancer.
R-HSA-6804757. Regulation of TP53 Degradation.
SIGNORiP85299.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline-rich protein 5
Alternative name(s):
Protein observed with Rictor-1
Short name:
Protor-1
Gene namesi
Name:PRR5
Synonyms:PROTOR1
ORF Names:PP610
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:31682. PRR5.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • TORC2 complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi55615.
OpenTargetsiENSG00000186654.
PharmGKBiPA144596392.

Polymorphism and mutation databases

BioMutaiPRR5.
DMDMi160016058.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003081621 – 388Proline-rich protein 5Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei252PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP85299.
PaxDbiP85299.
PeptideAtlasiP85299.
PRIDEiP85299.

PTM databases

iPTMnetiP85299.
PhosphoSitePlusiP85299.

Expressioni

Tissue specificityi

Most abundant in kidney and liver. Also highly expressed in brain, spleen, testis and placenta. Overexpressed in several colorectal tumors.2 Publications

Gene expression databases

BgeeiENSG00000186654.
ExpressionAtlasiP85299. baseline and differential.
GenevisibleiP85299. HS.

Organism-specific databases

HPAiHPA054072.

Interactioni

Subunit structurei

Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and RICTOR within the TORC2 complex.2 Publications

Protein-protein interaction databases

BioGridi120755. 5 interactors.
IntActiP85299. 14 interactors.
STRINGi9606.ENSP00000384848.

Structurei

3D structure databases

ProteinModelPortaliP85299.
SMRiP85299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 95Interaction with RICTORAdd BLAST86
Regioni188 – 218Interaction with RICTORAdd BLAST31

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 27Poly-Ala4

Sequence similaritiesi

Belongs to the PROTOR family.Curated

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00530000063981.
HOGENOMiHOG000290687.
HOVERGENiHBG067971.
InParanoidiP85299.
KOiK20411.
OMAiSPLVEYD.
OrthoDBiEOG091G0VPK.
PhylomeDBiP85299.
TreeFamiTF314826.

Family and domain databases

InterProiIPR013745. Bit61/PRR5.
IPR016159. Cullin_repeat-like_dom.
[Graphical view]
PfamiPF08539. HbrB. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P85299-1) [UniParc]FASTAAdd to basket
Also known as: alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRTLRRLKFM SSPSLSDLGK REPAAAADER GTQQRRACAN ATWNSIHNGV
60 70 80 90 100
IAVFQRKGLP DQELFSLNEG VRQLLKTELG SFFTEYLQNQ LLTKGMVILR
110 120 130 140 150
DKIRFYEGQK LLDSLAETWD FFFSDVLPML QAIFYPVQGK EPSVRQLALL
160 170 180 190 200
HFRNAITLSV KLEDALARAH ARVPPAIVQM LLVLQGVHES RGVTEDYLRL
210 220 230 240 250
ETLVQKVVSP YLGTYGLHSS EGPFTHSCIL EKRLLRRSRS GDVLAKNPVV
260 270 280 290 300
RSKSYNTPLL NPVQEHEAEG AAAGGTSIRR HSVSEMTSCP EPQGFSDPPG
310 320 330 340 350
QGPTGTFRSS PAPHSGPCPS RLYPTTQPPE QGLDPTRSSL PRSSPENLVD
360 370 380
QILESVDSDS EGIFIDFGRG RGSGMSDLEG SGGRQSVV
Length:388
Mass (Da):42,753
Last modified:October 23, 2007 - v1
Checksum:iFA6B90495623EF3F
GO
Isoform 2 (identifier: P85299-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-101: Missing.
     102-107: KIRFYE → MAPMPT

Show »
Length:287
Mass (Da):31,125
Checksum:i24E46CD123CA100E
GO
Isoform 3 (identifier: P85299-3) [UniParc]FASTAAdd to basket
Also known as: beta

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:379
Mass (Da):41,551
Checksum:i1A0E3E17802AFD85
GO
Isoform 4 (identifier: P85299-4) [UniParc]FASTAAdd to basket
Also known as: gamma

The sequence of this isoform differs from the canonical sequence as follows:
     1-95: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:293
Mass (Da):32,061
Checksum:i63D42183C35C9A0B
GO
Isoform 5 (identifier: P85299-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-5: MRTLR → MVCLELSQREAWGSGSPEKMPAQPEGLY

Note: No experimental confirmation available.
Show »
Length:411
Mass (Da):45,172
Checksum:i85B5B9159AB9350B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti280R → W in BAA90999 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_062230243V → M.Corresponds to variant rs36082900dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0016461 – 101Missing in isoform 2. 2 PublicationsAdd BLAST101
Alternative sequenceiVSP_0288841 – 95Missing in isoform 4. CuratedAdd BLAST95
Alternative sequenceiVSP_0288851 – 9Missing in isoform 3. Curated9
Alternative sequenceiVSP_0458831 – 5MRTLR → MVCLELSQREAWGSGSPEKM PAQPEGLY in isoform 5. 1 Publication5
Alternative sequenceiVSP_001648102 – 107KIRFYE → MAPMPT in isoform 2. 2 Publications6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000192 mRNA. Translation: BAA90999.1.
AK055848 mRNA. Translation: BAG51585.1.
AF177331 mRNA. Translation: AAG17975.1.
Z93244, Z98743 Genomic DNA. Translation: CAQ06711.1.
Z93244, Z98743 Genomic DNA. Translation: CAQ06720.1.
Z98743, Z93244 Genomic DNA. Translation: CAQ09256.1.
Z98743, Z93244 Genomic DNA. Translation: CAQ09263.1.
CH471138 Genomic DNA. Translation: EAW73353.1.
CH471138 Genomic DNA. Translation: EAW73355.1.
BC016921 mRNA. Translation: AAH16921.1.
BK005635 mRNA. Translation: DAA05654.1.
BK005636 mRNA. Translation: DAA05655.1.
BK005637 mRNA. Translation: DAA05656.1.
BK005638 mRNA. Translation: DAA05657.1.
BK005639 mRNA. Translation: DAA05658.1.
CCDSiCCDS14058.1.
CCDS14059.1. [P85299-3]
CCDS56232.1. [P85299-5]
CCDS74875.1. [P85299-4]
RefSeqiNP_001017528.1. NM_001017528.2. [P85299-3]
NP_001017529.1. NM_001017529.2. [P85299-4]
NP_001017530.1. NM_001017530.1. [P85299-4]
NP_001185650.1. NM_001198721.1. [P85299-5]
NP_056181.2. NM_015366.3. [P85299-3]
NP_851850.1. NM_181333.3. [P85299-1]
UniGeneiHs.102336.

Genome annotation databases

EnsembliENST00000006251; ENSP00000006251; ENSG00000186654. [P85299-3]
ENST00000336985; ENSP00000337464; ENSG00000186654. [P85299-1]
ENST00000403581; ENSP00000384848; ENSG00000186654. [P85299-5]
ENST00000611394; ENSP00000480357; ENSG00000186654. [P85299-3]
ENST00000617066; ENSP00000479623; ENSG00000186654. [P85299-4]
ENST00000624862; ENSP00000485597; ENSG00000186654. [P85299-4]
GeneIDi55615.
KEGGihsa:55615.
UCSCiuc003bew.2. human.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000192 mRNA. Translation: BAA90999.1.
AK055848 mRNA. Translation: BAG51585.1.
AF177331 mRNA. Translation: AAG17975.1.
Z93244, Z98743 Genomic DNA. Translation: CAQ06711.1.
Z93244, Z98743 Genomic DNA. Translation: CAQ06720.1.
Z98743, Z93244 Genomic DNA. Translation: CAQ09256.1.
Z98743, Z93244 Genomic DNA. Translation: CAQ09263.1.
CH471138 Genomic DNA. Translation: EAW73353.1.
CH471138 Genomic DNA. Translation: EAW73355.1.
BC016921 mRNA. Translation: AAH16921.1.
BK005635 mRNA. Translation: DAA05654.1.
BK005636 mRNA. Translation: DAA05655.1.
BK005637 mRNA. Translation: DAA05656.1.
BK005638 mRNA. Translation: DAA05657.1.
BK005639 mRNA. Translation: DAA05658.1.
CCDSiCCDS14058.1.
CCDS14059.1. [P85299-3]
CCDS56232.1. [P85299-5]
CCDS74875.1. [P85299-4]
RefSeqiNP_001017528.1. NM_001017528.2. [P85299-3]
NP_001017529.1. NM_001017529.2. [P85299-4]
NP_001017530.1. NM_001017530.1. [P85299-4]
NP_001185650.1. NM_001198721.1. [P85299-5]
NP_056181.2. NM_015366.3. [P85299-3]
NP_851850.1. NM_181333.3. [P85299-1]
UniGeneiHs.102336.

3D structure databases

ProteinModelPortaliP85299.
SMRiP85299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120755. 5 interactors.
IntActiP85299. 14 interactors.
STRINGi9606.ENSP00000384848.

PTM databases

iPTMnetiP85299.
PhosphoSitePlusiP85299.

Polymorphism and mutation databases

BioMutaiPRR5.
DMDMi160016058.

Proteomic databases

MaxQBiP85299.
PaxDbiP85299.
PeptideAtlasiP85299.
PRIDEiP85299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000006251; ENSP00000006251; ENSG00000186654. [P85299-3]
ENST00000336985; ENSP00000337464; ENSG00000186654. [P85299-1]
ENST00000403581; ENSP00000384848; ENSG00000186654. [P85299-5]
ENST00000611394; ENSP00000480357; ENSG00000186654. [P85299-3]
ENST00000617066; ENSP00000479623; ENSG00000186654. [P85299-4]
ENST00000624862; ENSP00000485597; ENSG00000186654. [P85299-4]
GeneIDi55615.
KEGGihsa:55615.
UCSCiuc003bew.2. human.

Organism-specific databases

CTDi55615.
DisGeNETi55615.
GeneCardsiPRR5.
HGNCiHGNC:31682. PRR5.
HPAiHPA054072.
MIMi609406. gene.
neXtProtiNX_P85299.
OpenTargetsiENSG00000186654.
PharmGKBiPA144596392.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4406. Eukaryota.
ENOG410XR4J. LUCA.
GeneTreeiENSGT00530000063981.
HOGENOMiHOG000290687.
HOVERGENiHBG067971.
InParanoidiP85299.
KOiK20411.
OMAiSPLVEYD.
OrthoDBiEOG091G0VPK.
PhylomeDBiP85299.
TreeFamiTF314826.

Enzyme and pathway databases

ReactomeiR-HSA-1257604. PIP3 activates AKT signaling.
R-HSA-389357. CD28 dependent PI3K/Akt signaling.
R-HSA-5218920. VEGFR2 mediated vascular permeability.
R-HSA-5674400. Constitutive Signaling by AKT1 E17K in Cancer.
R-HSA-6804757. Regulation of TP53 Degradation.
SIGNORiP85299.

Miscellaneous databases

ChiTaRSiPRR5. human.
GenomeRNAii55615.
PROiP85299.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186654.
ExpressionAtlasiP85299. baseline and differential.
GenevisibleiP85299. HS.

Family and domain databases

InterProiIPR013745. Bit61/PRR5.
IPR016159. Cullin_repeat-like_dom.
[Graphical view]
PfamiPF08539. HbrB. 1 hit.
[Graphical view]
SUPFAMiSSF74788. SSF74788. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPRR5_HUMAN
AccessioniPrimary (citable) accession number: P85299
Secondary accession number(s): B1AHF6
, B1AHG5, B3KP73, O75983, O95695, Q5BIW2, Q5EAJ8, Q5EAJ9, Q5XKJ6, Q96RW1, Q96RW2, Q9HA49, Q9HC46, Q9NSG0, Q9NVX8, Q9NXL1, Q9UH20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: October 23, 2007
Last modified: November 30, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.